NO一氧化氮分析套組 | Nitric Oxide assay kit 貨號K262-200 (ABCAM ab65328)
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Nitric Oxide一氧化氮分析套組 | 貨號K262-200 Inorganic Ions (ABCAM ab65328)
靈敏度為1.0 nmole nitrite/well或者 10 µM.
一氧化氮分析套組 貨號K262-200 | 200個分析 Nitric Oxide Colorimetric Assay Kit 。產品描述: 一氧化氮（NO）在神經傳遞、血管調節、免疫反應、與細胞凋亡中扮演重要角色。NO迅速被氧化為亞硝酸鹽和硝酸鹽其用於定量NO的產生。BioVision的的一氧化氮呈色分析試劑盒，提供簡單兩步驟，準確測量硝酸鹽總/亞硝酸鹽的含量。第一步使用硝酸還原酶將硝酸鹽轉化為亞硝酸鹽。第二步使用格里斯試劑，亞硝酸鹽轉化為深紫色偶氮化合物。azochromophore的量準確地反映樣品一氧化氮含量。此套組靈敏度為1.0 nmole nitrite/well或者 10 µM.。
Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune response and apoptosis. NO is rapidly oxidized to nitrite and nitrate which are used to quantitate NO production. BioVision’s Nitric Oxide Colorimetric Assay Kit provides an accurate, convenient measure of total nitrate/nitrite in a simple two-step process. The first step converts nitrate to nitrite utilizing nitrate reductase. The second step uses Griess Reagents to convert nitrite to a deep purple azo compound. The amount of the azochromophore accurately reflects nitric oxide amount in samples. The detection limit of the assay is approximately 1.0 nmole nitrite/well, or 10 µM.
細胞裂解物中測量的亞硝酸鹽和硝酸鹽顯示每 10 6 個細胞的數量（微摩爾）
生物製品中測量的亞硝酸鹽和硝酸鹽顯示濃度（微摩爾）
標準曲線（比色法）：減去背景讀數的重複平均值 (+/-SD)
| Cat # +Size | K262-200 (ABCAM ab65328) |
|---|---|
| Size | 200 assays |
| Detection Method | Absorbance (540 nm) |
| Species Reactivity | Mammalian |
| Applications | BioVision’s Nitric Oxide Colorimetric Assay Kit provides an accurate, convenient measure of total nitrate/nitrite in a simple two-step process. |
| Features & Benefits | • Simple two-step procedure; takes only ~1-1.5 hours • Fast and convenient • BioVision’s Nitric Oxide Colorimetric Assay Kit provides an accurate, convenient measure of total nitrate/nitrite. |
| Kit Components | • Assay Buffer • Enzyme cofactor • Enhancer • Nitrate Reductase • Nitrate Standard • Nitrite Standard • Griess Reagent R1 • Griess Reagent R2 • Microtiter Plate • Plate Cover |
引用文獻
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