BrdU細胞增殖測定試劑盒 | 貨號K306-1000

BrdU Cell Proliferation Assay Kit

5-溴-2-脫氧尿苷（5-bromo-2-deoxyuridine, BrdU）是嘧啶類似物 ( pyrimidine analog)。 它被 incorporated 到新合成的增殖細胞DNA (proliferating cells) 中，代替胸苷 (thymidine)。 Biovision的BrdU細胞增殖測定試劑盒(BrdU Cell Proliferation Assay Kit, k306) 使用小鼠抗BrdU抗體 (anti-BrdU antibody) 檢測摻入的BrdU。 使用抗小鼠HRP連接的二抗 ( anti-mouse HRP-linked secondary antibody) 來檢測與BrdU結合的抗BrdU抗體，然後加入TMB（HRP substrate）。 顯色程度與摻入細胞中的BrdU量成正比，可以直接用作細胞增殖 (cell proliferation) 的指標。 與其他細胞增殖測定相比，該試劑盒僅檢測增殖細胞而不檢測seeded cells。 這種高度敏感的非放射性試劑盒可檢測到少於50-100個增殖細胞 (proliferating cells)。

Detection Method	Absorbance (450 nm)
Species Reactivity	N/A
Applications	Detection and quantification of cell proliferation induced by growth factors, cytokines, mitogens, and nutrients. Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals. Determination of the inhibitory or stimulatory effects of various compounds on cell proliferation.
Features & Benefits	• Convenient & non-radioactive • High-throughput suitable • Highly-sensitive: detect as low as 50-100 proliferating cells. • Suitable for cell proliferation, chemotaxis and cytotoxicity
Kit Components	• BrdU (1000X) • Fixing/Denaturing Solution • BrdU Detection Antibody (300X) • Anti-mouse HRP-linked Antibody (2000X) • Antibody Diluent • Wash Buffer (10X) • TMB Substrate • Stop Solution

引用文獻

Ye Chen, Bromodomain and extraterminal proteins foster the core transcriptional regulatory programs and confer vulnerability in liposarcoma. Nat Commun., March 2019;  30903020.

Severa Bunda, CIC protein instability contributes to tumorigenesis in glioblastoma. Nat Commun., Feb 2019; 30737375.

Chen, Si-Le et al. (2016) Targeting CRMP‑4 by lentivirus‑mediated RNA interference inhibits SW480 cell proliferation and colorectal cancer growth, Exp Ther Med. 2016 Oct;12(4):2003-2008.