細胞表面蛋白分離試劑盒 | Cell Surface Protein Isolation Kit 貨號K295-10

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Cell Surface Protein Isolation Kit 細胞表面蛋白分離試劑盒 | 貨號K295
Simple and efficient method for isolation of cell surface proteins
產品描述:細胞表面蛋白(Cell surface proteins)在信號轉導( signal transduction),細胞粘附(cell adhesion),轉運(transport )中起主要作用,並作為診斷(diagnostic)和藥理學(pharmacological targets)靶點。 BioVision的細胞表面蛋白分離試劑盒提供了一種簡單有效的細胞表面蛋白分離方法。 在該方法中,首先用Sulfo-NHS-SS生物素( Sulfo-NHS-SS-Biotin), 胺反應性 (thiol-cleavable),硫醇可切割的生物素化試劑(thiol-cleavable)標記細胞。 隨後細胞裂解,使用鏈黴抗生物素蛋白珠(Streptavidin beads)分離標記的細胞表面蛋白質。 然後通過與DTT溶液孵育從珠粒釋放結合的蛋白質。 生物素化試劑是具有延伸間隔臂的細胞膜不可滲透的,以減少與鏈黴親和素結合相關的空間障礙。 這種方便的試劑盒提供了所有必需的組分,用於細胞表面蛋白的最佳標記和分離。
Cat # +Size | K295-10 |
Size | 10 reactions |
Detection Method | Western Blotting |
Applications | Isolated cell surface proteins can be used for various downstream applications such as Western Blotting or other structural and functional studies |
Features & Benefits | • Simple and efficient method for the isolation of cell surface proteins • Includes all required components for optimal labeling and isolation of the cell surface proteins |
Kit Components | • Quenching Solution • Lysis Buffer • Wash Buffer • Streptavidin Beads • PBS Tablet • TBS Tablet • Sulfo-NHS-SS-Biotin • DTT |