尿酸酶活性測定 | Uricase Activity Assay Kit (Fluorometric)貨號K734-100

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相關下載：	Uricase尿酸酶活性測定試劑盒操作手冊

特點

尿酸酶活性測定試劑盒 | 貨號K734-100

Uricase Activity Assay Kit (Fluorometric)

尿酸酶（(Uricase）是一種參與尿酸代謝的酶。(與因子無關的尿酸羥化酶，尿酸氧化酶（UO）； EC 1.7.3.3）。尿酸(Uric acid)是嘌呤代謝(purine metabolism)的最終產物，血液中高水平的尿酸會導致痛風(gout)。尿酸酶（(Uricase）存在於多種哺乳動物中，但人類卻不存在。它已被廣泛用於測量生物樣品中的尿酸濃度，重組尿酸酶（recombinant uricase ）已被用作預防腫瘤溶解綜合徵的藥物。 BioVision的尿酸酶活性測定試劑盒提供了一種快速簡便的方法來測量多種樣品中的尿酸酶(Uricase）活性。在這種測定中，尿酸酶(Uricase）將尿酸氧化，形成與探針反應的產物，從而產生螢光信號（Ex / Em = 535 / 587nm）。產生的螢光信號與樣品中存在的活性尿酶的量成正比。該試劑盒提供了靈敏且高通量的適應性測定，可以測量低至10 µU的生物樣品中尿酸酶(Uricase）的活性。

Cat # +Size	K734-100
Size	100 assays
Kit Summary	• Detection method– Fluorometric (535/587 nm) • Species reactivity- Mammalian/Plants • Applications- The kit can detect as low as 10 µU of Uricase
Detection Method	Detection method– Fluorometric (535/587 nm)
Species Reactivity	Eukaryotes
Applications	Rapid assessment of Uricase activity in mammalian and plant tissues
Features & Benefits	• Simple procedure; takes ~ 1 hour • Fast and convenient • The assay is sensitive, stable and high-throughput adaptable.
Kit Components	• Uricase Assay Buffer • Uricase Substrate • Uricase Probe (in DMSO) • Uricase Enzyme Mix • H2O2 Standard (0.88 M) • Uricase Positive Control

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