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GPx呈色分析套組 |Glutathione Peroxidase Assay 貨號K762-100 (Abcam ab102530)

代理廠牌:
原廠連結:
http://www.biovision.com
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GPx呈色分析套組操作手冊

特點

 GPx呈色分析套組Glutathione Peroxidase Activity Colorimetric Assay Kit   貨號K762-100  (Abcam ab102530)

0.5 mU/ml

biovision商品插圖

原理介紹Biovision提供一系列氧化相關分析套組;其中GPx呈色分析套組,以呈色Colorimetric  (340 nm)偵測;可用於細胞組織lysate細胞培養液、尿液、血漿、液體型式的來源。操作時間大約30-40分鐘。靈敏度0.5 mU/ml of GPx in samples

ELISA reader

ELISA儀器設備請按此  (標配405、450、492、630 nm濾片/最高可擴充至7濾片)(k788 λ= 570 nm)

GPx穀胱甘肽過氧化物酶(Glutathione Peroxidase, GPx,EC 1.11.1.9)在保護生物免受氧化損傷(oxidative damage)中,扮演重要作用。 GPx(Glutathione Peroxidase, GPx)將還原型穀胱甘肽(reduced glutathione, GSH)轉化為氧化型穀胱甘肽(oxidized glutathione , GSSG),同時將脂質氫過氧化物( lipid hydroperoxides) 還原為其相應的醇(alcohols)或將游離過氧化氫(free hydrogen peroxide )還原為水。 已經在不同的細胞位置和不同的底物特異性中發現了幾種同功酶。 低水平的GPx已經與自由基相關的疾病相關(free radical related disorders)。 在BioVision的穀胱甘肽過氧化物酶(Glutathione Peroxidase, GPx)活性測定中,GPx (Glutathione Peroxidase, GPx)還原枯烯氫過氧化物(Cumene Hydroperoxid),同時將GSH(reduced glutathione, GSH)氧化成GSSG(oxidized glutathione , GSSG)。 所產生的GSSG(oxidized glutathione , GSSG)被還原為GSH(reduced glutathione, GSH),通過GR (glutathione reductase) 消耗NADPH。 NADPH的降低(在340nm容易測量)與GPx(Glutathione Peroxidase, GPx)活性成比例。 該測定可用於測量血漿,紅細胞裂解物,組織勻漿和細胞裂解物中的所有穀胱甘肽依賴性過氧化物酶,樣品中的GPx(Glutathione Peroxidase, GPx)的檢測靈敏度為〜0.5mU / ml。

GSH_GSSG

GPx呈色分析套組 | K762-100內容物
• GPx Assay Buffer
• NADPH (lyophilized)
• Glutathione Reductase
• Glutathione (GSH, lyophilized)
• Cumene Hydroperoxide
• GPx Positive Control (lyophilized);

引用文獻

Zhou Zhou, Adipose‐Specific Lipin‐1 Overexpression Renders Hepatic Ferroptosis and Exacerbates Alcoholic Steatohepatitis in Mice. Hepatol Commun, May 2019; 31061954.
Long Chen, LncRNA GAS5 regulates redox balance and dysregulates the cell cycle and apoptosis in malignant melanoma cells. J Cancer Res Clin Oncol, Dec 2018; 30569211.
Jesmin Ara, et al., (2018) Hydrogen Water Drinking Exerts Antifatigue Effects in Chronic Forced Swimming Mice via Antioxidative and Anti-Inflammatory Activities, BioMed Research International, Apr. 2018, 29850492
Musabayane, C. T. et al., (2017) Kidney Function in P Berghei-Infected Sprague-Dawley Rats Following Treatment with Transdermally Delivered Syzygium-aromaticum Derived Oleanolic Acid, J Endocrinology and Thyroid Res, Apr.2017, 1(3)
Sibiya, HP et al., (2017) Kidney Function in P. Berghei-Infected Sprague-Dawley Rats Following Treatment with Transdermally Delivered Syzygium-aromaticum Derived Oleanolic Acid, J Endocrinol Thyroid Res, 2017, 1(3): 555565
K762
 Catalog #  Product Name+  Size
 K671-100  Ascorbic Acid Colorimetric Assay Kit II (FRASC)  100 assays
 K661-100  Ascorbic Acid Colorimetric/Fluorometric Assay Kit  100 assays
 K773-100  Catalase Activity Colorimetric/Fluorometric Assay Kit  100 assays
 K620-100  Ethanol Colorimetric/Fluorometric Assay Kit  100 assays
 K504-100  EZCell™ Glutathione Detection Kit (Blue Fluorescence)   100 assays
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 K264-100  Glutathione (GSH/GSSG/Total) Fluorometric Assay Kit  100 assays
 K261-100  Glutathione Colorimetric Assay Kit  100 assays
 K251-100  Glutathione Fluorometric Assay Kit  100 assays
 K762-100  Glutathione Peroxidase Activity Colorimetric Assay Kit  100 assays
 K761-200  Glutathione Reductase Activity Colorimetric Assay Kit  200 assays
 K263-100  GST Colorimetric Activity Assay Kit  100 assays
 K260-100  GST Fluorometric Activity Assay Kit  100 assays
 K336-100  HAT (H4) Activity Fluorometric Assay Kit  100 assays
 K334-100  HAT Activity Fluorometric Assay Kit  100 assays
 K332-100  HAT Activity Colorimetric Assay Kit  100 assays
 K331-100  HDAC Activity Colorimetric Assay Kit  100 assays
 K330-100  HDAC Activity Fluorometric Assay Kit  100 assays
 K340-100  HDAC Inhibitor Drug Screening Kit (Fluorometric)  100 assays
 K265-200  Hydrogen Peroxide Assay Kit  200 assays
 K712-100  Inosine Fluorometric Assay Kit  100 assays
 K339-100  InSitu HDAC Activity Fluorometric Assay Kit  100 assays
 K204-200  Intracellular Hydrogen Peroxide Detection Kit (cell-based)  200 assays
 K739-100  Lipid Peroxidation (MDA) Colorimetric/Fluorometric Assay Kit  100 assays
 K744-100  Myeloperoxidase (MPO) Colorimetric Activity Assay Kit  100 assays
 K745-100  Myeloperoxidase (MPO) Fluorometric Activity Assay Kit  100 assays
 K746-200  Myeloperoxidase (MPO) Inhibitor Screening Kit (Fluorometric)  200 assays
 K747-100  Myeloperoxidase (MPO) Peroxidation Activity Assay Kit  100 assays
 K262-200  Nitric Oxide Colorimetric Assay Kit  200 assays
 K252-200  Nitric Oxide Fluorometric Assay Kit  200 assays
 K205-100  Nitric Oxide Synthase (NOS) Activity Assay Kit (Colorimetric)   100 assays
 K206-100  Nitric Oxide Synthase (NOS) Activity Assay Kit (Fluorometric)  100 assays
 K208-100  Nitric Oxide Synthase (NOS) Inhibitor Screening Kit (Fluorometric)  100 assays
 K4926-100  NQO1 (human intracellular) ELISA Kit  100 assays
 K772-100  Peroxidase Activity Colorimetric/Fluorometric Assay Kit  100 assays
 K840-100  Protein Disulfide Isomerases (PDI) Inhibitor Screening Kit (Fluorometric)  100 assays
 K335-100  Superoxide Dismutase (SOD) Activity Assay Kit  100 assays
 K763-100  Thioredoxin Reductase Activity Colorimetric Assay Kit  100 assays
 K274-100  Total Antioxidant Capacity (TAC) Colorimetric Assay Kit  100 assays
 K608-100  Uric Acid Colorimetric/Fluorometric Assay Kit  100 assays
 K710-100  Xanthine Oxidase Activity Colorimetric/Fluorometric Assay Kit  100 assays

smartSpectrumlab 102-T0100-01

 

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