碳水化合物(Carbohydrates)檢測 |  貨號K2049-100 (abcam ab284543)

High Sensitivity Carbohydrate Assay Kit (Colorimetric)

碳水化合物(Carbohydrates)是許多食品中最重要的成分之一，也是所有活生物體中存在的最豐富的生物分子。 在食品和飲料產品，農產品(agricultural products)，醫藥產品(medicinal products)，疫苗藥品(vaccine pharmaceuticals等)許多領域，總碳水化合物(carbohydrate)含量的定量分析是必不可少的分析程序。BioVision的高靈敏度碳水化合物測定試劑盒是一種簡單，靈敏，耐用的測定碳水化合物的方法，即使在 低濃度存在。 該測定法可用於定量測定任何含有中性糖(neutral sugars)和醣醛酸(uronic acids的)碳水化合物。 該反應是苯酚-硫酸方法(phenol-sulfuric acid method)的改進。 在該測定中，碳水化合物被水解並轉化為羥基糠醛(hydroxyfurfural)和糠醛(furfural,)，然後它們與高度敏感的顯影劑反應形成有色絡合物，在500 nm處用分光光度法檢測。

Cat # +Size	K2049-100
Size	100 assays
Kit Summary	BioVision’s High Sensitivity Carbohydrate Assay Kit is a simple, sensitive, robust assay for the quantification of carbohydrates even when present in low concentration. The assay can be used for the quantitative determination of any carbohydrates that contain neutral sugars and uronic acids. The reaction is a modification of phenol-sulfuric acid method. In this assay, carbohydrates are hydrolyzed and converted to hydroxyfurfural and furfural, which then react with a highly sensitive developer to form a colored complex, detected spectrophotometrically at 500 nm.
Detection Method	Absorbance at 500 nm
Species Reactivity	All
Applications	Quantitation of total carbohydrate in vaccines and various samples.
Features & Benefits	• The reaction is a modification of phenol-sulfuric acid method. • A simple, sensitive, robust assay for the quantification of carbohydrates even when present in low concentration.
Kit Components	• Carbohydrate Developer • Standard (D-Glucose, 2 mg/ml)
Storage Conditions	4°C
Shipping Conditions	Gel Pack
USAGE	For Research Use Only! Not For Use in Humans.

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