Phosphatidic acid磷脂酸檢測試劑盒 | biovision貨號K748-100

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Phosphatidic acid磷脂酸檢測試劑盒 | 貨號K748-100
磷脂(Phospholipids)是含有磷酸基團的兩親分子(amphiphilic molecules),既是生物膜(biological membranes)的重要組成部分,又是許多生物途徑中的重要信號傳遞劑。磷脂酸(Phosphatidic acid , PA)是最簡單的磷脂,由甘油主鍊(glycerol backbone)和連接在碳1和2上的脂肪酸以及與碳3結合的磷酸基團組成。磷脂酸(Phosphatidic acid , PA)是合成更複雜的磷脂(如磷脂酰膽鹼 (phosphatidylcholine),磷脂酰乙醇胺(phosphatidylethanolamine))的前體。磷脂酰絲氨酸(phosphatidylserine)作為質膜(plasma membrane)的結構成分。磷脂酸(Phosphatidic acid)本身存在於質膜(plasma membrane)中,使該膜具有高曲率(high curvature)和淨負電荷(net negative charge)。另外,它是重要的信號脂質。它通過G蛋白偶聯受體(G-protein coupled receptor)起作用,並參與生長調節(growth regulation)和有絲分裂(mitosis)。 BioVision的磷脂酸檢測試劑盒(BioVision’s phosphatidic acid assay kit, k748)是一種基於板的酶檢測法 (plate-based enzymatic assay),用於定量檢測細胞和組織中的PA磷脂酸(Phosphatidic acid , PA)。 PA Converter水解 PA磷脂酸(Phosphatidic acid , PA)形成中間體,在存在顯影劑和酶混合物的情況下,該中間體將非螢光探針轉換為可以定量的螢光產物(Ex / Em = 535/587 nm)。轉換器對PA具有特異性,不會水解其他磷脂(例如具有更複雜head groups的磷脂,例如磷脂酰膽鹼(phosphatidylcholine)或心磷脂(cardiolipin)),可直接定量PA磷脂酸(Phosphatidic acid , PA)。該試劑盒每孔可檢測低至40 pmol PA(Phospholipids)。


| Cat # +Size | K748-100 |
| Size | 100 assays |
| Kit Summary | • Detection method- Fluorescence (Ex/Em 535/587 nm) • Sample type- Cell and/or tissue lipid extracts • Species reactivity- Mammalian • Applications- This assay provides a convenient and non-ELISA alternative to detect phosphatidic acid amount in biological samples. |
| Detection Method | Fluorescence (Ex/Em 535/587 nm) |
| Species Reactivity | Mammalian |
| Applications | This assay provides a convenient and non-ELISA alternative to detect phosphatidic acid amount in biological samples. |
| Features & Benefits | Simple, fast, does not require lenghty incubation times |
| Kit Components | • PA Assay Buffer • PA Converter • PA Developer • PA Enzyme Mix • PicoProbeTM (in DMSO) • PA Standard (1 mM) |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
乾浴加熱水浴槽 | Finnpipette F3 微量分注器 | 試管振盪器 Vortex Mixer | Genereach迷你型微量離心機 | ELISA儀器




