醋酸比色分析試劑盒 | 貨號K658-100

Acetate Colorimetric Assay Kit

產品介紹: 醋酸鹽(Acetate)是生物合成( biosynthesis)的重要組成部分。 在生物體(living organisms)中，蛋白質的乙醯化( acetylation)/去乙醯化(deacetylation)對於其功能的翻譯後(post-translational regulation)調節是至關重要的。 當與CoA結合時，乙醯輔酶A(Acetyl-CoA)是碳水化合物( carbohydrates)和脂肪( fats) 代謝的中心。 它也參與生物化合物如神經遞質乙醯膽鹼(neurotransmitter acetylcholine) 的合成。在Biovision的醋酸鹽分析試劑盒(Acetate Assay Kit, K658) 中，醋酸鹽在醋酸酶混合物和醋酸鹽底物混合物的存在下轉化為中間體。該中間體將無色探針還原成在450nm具有強吸光度的有色產物。醋酸比色法檢測試劑盒(Acetate Colorimetric Assay Kit, K658) 快速，簡單，靈敏。 在各種樣品中可以檢測到少於20μM的醋酸鹽 (20 µM Acetate) 。

Cat # +Size	K658-100
Size	100 assays
Detection Method	Absorbance (450 nm)
Applications	The kit can detect less than 20 μM of Acetate in various samples.
Features & Benefits	• Easy-to-use • Rapid, convenient and sensitive • Kit contains all necessary reagents for accurate measurement of Acetate levels
Kit Components	• Acetate Assay Buffer • Acetate Enzyme Mix (Lyophilized) • ATP (Lyophilized) • Acetate Substrate Mix (Lyophilized) • Probe (Lyophilized) • Acetate Standard (Lyophilized)

引用文獻

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McNelis et al., GPR43 Potentiates β-Cell Function in Obesity.  Diabetes, Sep 2015; 64: 3203 – 3217.

Kaji et al., SCFA transport in rat duodenum. Am J Physiol Gastrointest Liver Physiol, Feb 2015; 308: G188 – G197.