PI-Propidium Iodide 死細胞核酸染劑 |CAS#25535-16-4 Biovision貨號1056 (ABCAM ab14083)
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特點
Propidium Iodide 死細胞核酸染劑 | 貨號1056 (ABCAM ab14083)
3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide, PI 3,8-二氨基-5-[3-(二乙基家氨基)丙基]-6-苯基啡啶二碘 螢光染料PI（碘化丙啶）是一種可對DNA染色的細胞核染色試劑，常用於細胞凋亡檢測，英文全稱是Propidium Iodide。它是一種溴化乙啶的類似物，在嵌入雙鏈DNA後釋放紅色螢光。儘管PI不能通過活細胞膜，但卻能穿過破損的細胞膜而對核染色。PI經常被用來與Calcein-AM或者FDA等螢光探針一起使用，能同時對活細胞和死細胞染色。PI-DNA複合物的激發和發射波長分別為535 nm和615 nm。
貨號1056-1, 為提供1 ml (1 mg/ml in PBS)包裝; 稀釋4倍使用, 每次加入量為1ul。大約使用4000次；但每個細胞需最佳化調整。The PI reagent should be diluted to 250 µg/ml with PBS before use. Add 1 µl per annexin V assay, as described in the user manuals (Cat.# 1001-1000, 1002-1000, 1004-1000).
| Cat # +Size | 1056-1 |
|---|---|
| Size | 1 ml |
| Alternate Name | 3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide, PI |
| Appearance | Liquid |
| Formulation | PBS ( 1 mg/ml) |
| CAS # | 25535-16-4 |
| Molecular Formula | C₂₇H₃₄I₂N₄ |
| Molecular Weight | 668.39 |
| SMILES | CC[N+](C)(CC)CCC[N+]1=C2C=C(C=CC2=C3C=CC(=CC3=C1C4=CC=CC=C4)N)N.[I-].[I-] |
| InChi | InChI=1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1 |
| InChi Key | XJMOSONTPMZWPB-UHFFFAOYSA-M |
| PubChem CID | 104981 |
| MDL Number | MFCD00011921 |
| Handling | Protect from light and moisture |
| Storage Conditions | 4°C |
| Shipping Conditions | Gel Pack |
| Catalog # |  其它相關染劑產品 |
Size |
| 2757-25 | 5-CFDA | 25 mg |
| 2757-100 | 5-CFDA | 100 mg |
| 2758-25 | 6-CFDA | 25 mg |
| 2758-100 | 6-CFDA | 100 mg |
| 2725-1 | 7-Aminoactinomycin D | 1 mg |
| 2725-5 | 7-Aminoactinomycin D | 5 mg |
| 2178-25 | bisBenzimide H 33258 | 25 mg |
| 2178-100 | bisBenzimide H 33258 | 100 mg |
| 2179-25 | bisBenzimide H 33342 | 25 mg |
| 2179-100 | bisBenzimide H 33342 | 100 mg |
| 2588-100 | Congo Red | 100 mg |
| 2588-500 | Congo Red | 500 mg |
| B1098-5 | DAPI | 5 mg |
| B1098-25 | DAPI | 25 mg |
| 9548-100 | ER-Hunt Green | 100 µg |
| 9549-100 | ER-Hunt Red | 100 µg |
| 1203-10 | Ethidium Bromide | 10 ml |
| 2586-1G | Evans Blue Tetrasodium | 1 g |
| 2727-250 | EZSolution™ 7-Aminoactinomycin D | 250 µg |
| 2727-1000 | EZSolution™ 7-Aminoactinomycin D | 1 mg |
| 2664-5 | Methoxy-X04 | 5 mg |
| 2664-25 | Methoxy-X04 | 25 mg |
| 1056-1 | Propidium Iodide | 1 ml |
| 2587-100 | Rose bengal | 100 mg |
| 2587-500 | Rose bengal | 500 mg |
| 1209-10 | Trypan Blue (0.4%) | 10 ml |