膽鹼/乙醯膽鹼定量試劑盒| Choline/Acetylcholine Kit貨號K615-100 (ABCAM ab65345)

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原廠連結：	http://www.biovision.com
相關下載：	膽鹼/乙醯膽鹼定量呈色/螢光試劑盒操作手冊

特點

膽鹼/乙醯膽鹼定量呈色/螢光試劑盒（螢光測定）| 貨號K615-100

產品介紹: 膽鹼和乙醯膽鹼在許多生物過程中起重要作用。 BioVision的膽鹼/乙醯膽鹼定量試劑盒提供了一種用比色法或螢光法對膽鹼和乙醯膽鹼進行定量的簡單而敏感的手段。在測定中，游離膽鹼通過中間體甜菜鹼醛氧化成甜菜鹼。該反應產生與膽鹼探針反應產生顏色（λ= 570nm）和螢光（Ex / Em 535/587nm）的產物。通過向反應中加入乙醯膽鹼酯酶，可以將乙醯膽鹼轉化為膽鹼。該試劑盒可檢測血液，細胞，培養基，發酵培養基等各種生物樣品中的膽鹼和乙醯膽鹼（總膽鹼不飽和膽鹼）。無需進行預處理或純化樣品。該試劑盒可以檢測10 pmol〜5 nmol的膽鹼或乙醯膽鹼。

Cat # +Size	K615-100
Size	100 assays
Detection Method	Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
Species Reactivity	Mammalian
Applications	The kit can detect 10 pmol to ~5 nmol of choline or acetylcholine.
Features & Benefits	• Simple procedure; takes ~40 minutes • Fast and convenient • Kit provides all necessary buffers and reagents for a accurate assay of Choline/Acetylcholine in various biological samples
Kit Components	• Choline Assay Buffer • Choline Probe • Choline Enzyme Mix • Acetylcholinesterase • Choline Standard (5 µmol)

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