丙氨酸氨基轉移酶（ALT或SGPT）檢測試劑盒 | ALT or SGPT Activity Colorimetric/Fluorometric Assay Kit貨號K752-100
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丙氨酸氨基轉移酶（ALT或SGPT）活性比色/螢光測定試劑盒 | 貨號K752-100
Alanine Aminotransferase (ALT or SGPT) Activity Colorimetric/Fluorometric Assay Kit
丙氨酸轉氨酶（ALT）是一種轉氨酶（EC 2.6.1.2）。它也被稱為血清穀丙轉氨酶（SGPT）或丙氨酸轉氨酶（ALAT）。 ALT存在於血清和各種身體組織中，但通常與肝臟有關。它催化反應：α-酮戊二酸+丙氨酸⇌谷氨酸+丙酮酸它通常在臨床上作為診斷肝功能測試的一部分來測定以確定肝臟健康。診斷上，幾乎總是以單位/升（U / L）來衡量。在BioVision的ALT檢測試劑盒中，ALT催化氨基從丙氨酸到ket-酮戊二酸的轉移，該可逆轉氨反應的產物是丙酮酸和谷氨酸。在將幾乎無色的探針同時轉換成顏色（λmax= 570nm）和螢光（Ex / Em = 535 / 587nm）的反應中檢測到丙酮酸鹽。該試劑盒提供了一種快速，簡單，靈敏和可靠的檢測方法，適用於ALT的高通量活性檢測，檢測限為每孔10 mU。
ELISA儀器設備請按此 (標配405、450、492、630 nm濾片/最高可擴充至7濾片）（k752 λ= 570 nm）
| Cat # +Size | K752-100 |
| Size | 100 assays |
| Detection Method | Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) |
| Species Reactivity | Mammalian |
| Applications | Evaluation of pentose phosphate pathway |
| Features & Benefits | • Simple procedure • Fast and convenient • Sensitive assays for measuring ALT activity in various biological samples and the kit is also suitable for high throughput analyses. |
| Kit Components | • ALT Assay Buffer • OxiRed Probe (in DMSO) • ALT Enzyme Mix • ALT Substrate • Pyruvate Standard (100 nmol/µl) • ALT positive control |
白蛋白（BCG）檢測 | （AST）活性測定試劑 | ALT/SGPT）活性比色螢光測定試劑 | Ammonia測定試劑 | 膽紅素分析試劑盒
引用文獻
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