JC-1 粒線體染劑 |mitochondrial dye測量粒線體膜電位CAS #3520-43-2 貨號1130-5
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JC-1 粒腺體染劑 | 貨號1130-5 ( 5, 5’ ,6, 6’ -tetrachloro-1, 1’ ,3, 3’ -tetraethyl-benzimidazolylcarbocyanine Iodide_Catalog#: 1130-5 | Size: 5 mg
JC-1 染劑是一種粒線體染劑(mitochondrial dye)。JC-1染劑可以測量粒線體膜電位，染出粒線體膜電位失衡現象。其原理為: 當帶正電的JC-1染劑進入細胞中，會被粒線體膜上的負性電場所吸引並聚集形成二聚體，在螢光顯微鏡下發出橘組色螢光；若是粒線體上膜電位消失，使JC-1染劑無法被膜上電場所吸引，而散佈在細胞中，並呈現單體狀態，在螢光顯微鏡下發出綠色螢光。JC-1染劑可以用來觀察細胞凋亡現象，在健康的細胞中，發出紅色螢光；當細胞胞凋亡發生時，發出綠色染光。
Description: JC-1 is a mitochondrial dye that stains mitochondria in living cells in a membrane potential-dependent fashion. JC-1 monomer is in equilibrium with so called J-aggregates, which are favored at higher mitochondrial membrane potential. The monomer JC-1 has green fluorescence (lem = 527 nm), while the J-aggregates have red fluorescence (lem = 590 nm). Therefore, it has been possible to use fluorescence ratioing technique to study mitochondrial membrane potential. JC-1 is particularly useful for apoptosis studies. In apoptotic cells, the dye stays in the cytoplasm and fluoresces green, while in healthy cells, the dye aggregates in the mitochondria and fluoresces red.
| Cat # +Size | 1130-5 |
|---|---|
| Size | 5 mg |
| Alternate Name | 5,5’,6,6’-tetrachloro-1,1’,3,3’-tetraethyl-benzimidazolylcarbocyanine Iodide |
| Appearance | Brown red solid |
| CAS # | 3520-43-2 |
| Molecular Formula | C₂₅H₂₇Cl₄IN₄ |
| Molecular Weight | 652.23 |
| Purity | >98% by HPLC |
| Solubility | DMSO and DMF |
| SMILES | CCN1C2=CC(=C(C=C2[N+](=C1C=CC=C3N(C4=CC(=C(C=C4N3CC)Cl)Cl)CC)CC)Cl)Cl.[I-] |
| InChi | InChI=1S/C25H27Cl4N4.HI/c1-5-30-20-12-16(26)17(27)13-21(20)31(6-2)24(30)10-9-11-25-32(7-3)22-14-18(28)19(29)15-23(22)33(25)8-4;/h9-15H,5-8H2,1-4H3;1H/q+1;/p-1 |
| InChi Key | FYNNIUVBDKICAX-UHFFFAOYSA-M |
| PubChem CID | 5492929 |
| MDL Number | MFCD00188475 |
| Handling | Protect from light and moisture |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| Catalog# | Product Name | Size |
| 1130-5 | JC-1 | 5 mg |
| K250-100 | MitoCapture™ Mitochondrial Apoptosis Detection Fluorometric Kit | 100 assays |
| K250-25 | MitoCapture™ Mitochondrial Apoptosis Detection Fluorometric Kit | 25 assays |
