JC-1 粒線體染劑 |mitochondrial dye測量粒線體膜電位CAS #3520-43-2 貨號1130-5
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JC-1 粒腺體染劑 | 貨號1130-5 ( 5, 5’ ,6, 6’ -tetrachloro-1, 1’ ,3, 3’ -tetraethyl-benzimidazolylcarbocyanine Iodide_Catalog#: 1130-5 | Size: 5 mg
JC-1 染劑是一種粒線體染劑(mitochondrial dye)。JC-1染劑可以測量粒線體膜電位,染出粒線體膜電位失衡現象。其原理為: 當帶正電的JC-1染劑進入細胞中,會被粒線體膜上的負性電場所吸引並聚集形成二聚體,在螢光顯微鏡下發出橘組色螢光;若是粒線體上膜電位消失,使JC-1染劑無法被膜上電場所吸引,而散佈在細胞中,並呈現單體狀態,在螢光顯微鏡下發出綠色螢光。JC-1染劑可以用來觀察細胞凋亡現象,在健康的細胞中,發出紅色螢光;當細胞胞凋亡發生時,發出綠色染光。
Description: JC-1 is a mitochondrial dye that stains mitochondria in living cells in a membrane potential-dependent fashion. JC-1 monomer is in equilibrium with so called J-aggregates, which are favored at higher mitochondrial membrane potential. The monomer JC-1 has green fluorescence (lem = 527 nm), while the J-aggregates have red fluorescence (lem = 590 nm). Therefore, it has been possible to use fluorescence ratioing technique to study mitochondrial membrane potential. JC-1 is particularly useful for apoptosis studies. In apoptotic cells, the dye stays in the cytoplasm and fluoresces green, while in healthy cells, the dye aggregates in the mitochondria and fluoresces red.
Cat # +Size | 1130-5 |
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Size | 5 mg |
Alternate Name | 5,5’,6,6’-tetrachloro-1,1’,3,3’-tetraethyl-benzimidazolylcarbocyanine Iodide |
Appearance | Brown red solid |
CAS # | 3520-43-2 |
Molecular Formula | C₂₅H₂₇Cl₄IN₄ |
Molecular Weight | 652.23 |
Purity | >98% by HPLC |
Solubility | DMSO and DMF |
SMILES | CCN1C2=CC(=C(C=C2[N+](=C1C=CC=C3N(C4=CC(=C(C=C4N3CC)Cl)Cl)CC)CC)Cl)Cl.[I-] |
InChi | InChI=1S/C25H27Cl4N4.HI/c1-5-30-20-12-16(26)17(27)13-21(20)31(6-2)24(30)10-9-11-25-32(7-3)22-14-18(28)19(29)15-23(22)33(25)8-4;/h9-15H,5-8H2,1-4H3;1H/q+1;/p-1 |
InChi Key | FYNNIUVBDKICAX-UHFFFAOYSA-M |
PubChem CID | 5492929 |
MDL Number | MFCD00188475 |
Handling | Protect from light and moisture |
Storage Conditions | -20°C |
Shipping Conditions | Gel Pack |
Catalog# | Product Name | Size |
1130-5 | JC-1 | 5 mg |
K250-100 | MitoCapture™ Mitochondrial Apoptosis Detection Fluorometric Kit | 100 assays |
K250-25 | MitoCapture™ Mitochondrial Apoptosis Detection Fluorometric Kit | 25 assays |