PicoProbe™磷酸二氫丙酮（DHAP）螢光分析試劑盒 | 貨號K673-100

PicoProbe™ Dihydroxyacetone Phosphate (DHAP) Fluorometric Assay Kit

產品描述：Dihydroxyacetone Phosphate二羥基丙酮磷酸酯（DHAP）是脂質生物合成和糖酵解的重要中間體。在糖酵解中，果糖-1,6-二磷酸酯通過醛縮酶轉化為DHAP和甘油醛-3-磷酸（GAP）。 DHAP和GAP都充當細胞內丙糖磷酸鹽庫。通過丙糖磷酸異構酶（TPI）可將DHAP進一步轉化為GAP。在人類中，TPI缺陷是一種罕見的常染色體疾病。它引起hemolytic anemia溶血性貧血，neurological diseases神經系統疾病，甚至由於糖酵解途徑的阻塞和DHAP在紅細胞中的積累而導致死亡。 Biovision的PicoProbe™二羥基丙酮磷酸鹽測定試劑盒適用於測量各種樣品中常見的低水平DHAP。在該試劑盒中，TPI將DHAP轉化為GAP，經歷一系列反應並減少PicoProbe™以產生螢光。產生的螢光強度與磷酸二羥基丙酮的量成正比。產品操作簡單、靈敏且易於使用的檢測試劑盒可檢測多種樣品中低至0.5μM的磷酸二羥丙酮。

Cat # +Size	K673-100
Size	100 assays
Detection Method	Fluorescence (Ex/Em = 535/587 nm)
Applications	Measurement of Dhydroxyacetone Phosphate in various tissues/cells Analysis of metabolic pathways such as glycolysis, Calvin cycle in plants, methylglyoxal pathway and lipid biosynthesis Mechanistic study of human TPI deficiency
Features & Benefits	• Simple, Sensitive & Rapid protocol • Highly-Sensitive: Detects as low as 0.5 µM DHAP
Kit Components	• DHAP Assay Buffer • PicoProbe™ (in DMSO) • DHAP Enzyme Mix • DHAP Developer • DHAP Standard

 

 

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