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GRx 呈色分析套組 | Glutathione Reductase Colorimetric Assay 貨號K761-200 Abcam ab83461

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Glutathione Reductase 呈色操作手冊

特點

 Glutathione Reductase Activity Colorimetric Assay Kit呈色分析套組 |貨號K761-200 Abcam ab83461

原理介紹Biovision提供一系列氧化相關分析套組;其中Glutathione Reductase呈色分析套組,以吸收光Absorbance (405 nm)偵測;可用於細胞組織懸浮培養液、尿液、血漿、erythrocyte lysates或血清之多種液體型式的樣品來源。操作時間大約30-40分鐘。靈敏度0.1 – 40 mU/ml GR in various samples

穀胱甘肽還原酶 Glutathione Reductase (GR,EC1.8.1.7)催化氧化型穀胱甘肽oxidized glutathione (GSSG) NADPH-dependent還原型穀胱甘肽glutathione (GSH)。在GSH氧化還原循環(GSH redox cycle)中起扮演重要角色,維持足夠水平的還原型GSH (reduced GSH)。高GSH / GSSG比值,對於防止氧化oxidative stress是必需的。 BioVision Glutathione Reductase Assay Kit穀胱甘肽還原酶分析試劑盒,是一種用於測量生物樣品中GR (Glutathione Reductase) 活性的分析套組;具高靈敏度、簡單、直接和HTS之呈色分析法。在測定中,Glutathione Reductase GR將GSSG還原為GSH,其與5,5-二硫代雙(2-硝基苯甲酸)(DTNB)反應以產生TNB2-(黃色,λmax= 405nm),以吸收光測量Glutathione Reductase含量。 該測定法可以在各種樣品中檢測,其靈敏度為0.1-40mU / ml GR

K761

相關產品 觸酶Catalase活性分析 | 骨髓過氧化酶(Myeloperoxidase) 呈色分析 |Hydrogen Peroxide分析套組  | 總抗氧化物呈色分析套組

Glutathione Reductase 呈色分析套組 | K761-200內容物
• GR Assay Buffer
• 3% H₂O₂
• Catalase
• TNB Standard (5 mM)
• DTNB
• NADPH-GNERAT™
• GSSG
• GR Positive Control (10 mU)

引用文獻

Jiao Jiao Zhang, et al., (2018) MicroRNA-7450 regulates non-thermal plasma-induced chicken Sertoli cell apoptosis via adenosine monophosphate-activated protein kinase activation, Scientific Reports, Jun. 2018, 29884805
Parrillo, Lucia et al. (2017) Olive mill wastewater-enriched diet positively affects growth, oxidative and immune status and intestinal microbiota in the crayfish, Astacus leptodactylus, Aquaculture. 2017 Apr;473: 161-168
Seki, Keisuke et al. (2016) Varying butyric acid amounts induce different stress- and cell death-related signals in nerve growth factor-treated PC12 cells: implications in neuropathic pain absence during periodontal disease progression, Apoptosis. 2016 Jun;21(6):699-707.
Horta et al., Organ-coordinated response of early post-germination mahogany seedlings to drought. Tree Physiol, Apr 2014; 10.1093/treephys/tpu017.

Glutathione Reductase相關之產品

 Catalog #  Product Name+  Size
 K671-100  Ascorbic Acid Colorimetric Assay Kit II (FRASC)  100 assays
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 K763-100  Thioredoxin Reductase Activity Colorimetric Assay Kit  100 assays
 K274-100  Total Antioxidant Capacity (TAC) Colorimetric Assay Kit  100 assays
 K608-100  Uric Acid Colorimetric/Fluorometric Assay Kit  100 assays
 K710-100  Xanthine Oxidase Activity Colorimetric/Fluorometric Assay Kit  100 assays

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