ALDH醛脫氫酶活性檢測 | Aldehyde Dehydrogenase Activity Colorimetric Assay Kit貨號K731-100

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Aldehyde Dehydrogenase Activity Colorimetric Assay Kit
NAD依賴性醛脫氫酶( NAD-dependent Aldehyde Dehydrogenase, ALDH)在細胞解毒 ( cellular detoxification) 中扮演至關重要的作用。它氧化各種醛類 ( aldehydes) 並生成相應的羧酸 (carboxyolic acid。 ALDH已經在每一個細胞比較中找到。根據其結構( structure) 和功能( function),ALDH在哺乳動物中包含3個主要類別:1類( Class 1) 和3類( Class 3) (腫瘤形式)位於胞質 (cytosol ) 溶膠中並且包括組成型(constitutive) 和誘導型(induced forms);第2類 ( Class 2) 位於線粒體( mitochondria ) 中,僅作為組成型存在(constitutive form) 。在人類中,ALDH超家族 (ALDH superfamily ) 由19個基因組成。 ALDH基因的突變(功能喪失)引起人類疾病,如II型高脯氨酸血症 (Type II hyperprolinemia),吡哆醇依賴性癲癇發作(pyridoxine-dependent seizure ) 和高氨血症(hyperammonemia) 。最近的研究顯示,ALDH活性增加導致幾種類型的惡性腫瘤 (malignancies) ,作為癌症幹細胞標誌物 (cancer stem cell marker )並與不良預後 (poor prognosis)具關聯性。因此早期檢測ALDH活性水平可以預測並指導治療策略。 BioVision醛脫氫酶(ALDH)活性檢測試劑盒是一種簡單,快速和可靠的定量ALDH酶活性的方法。在該測定中,乙醛被ALDH氧化,產生NADH,然後將無色探針還原成在450nm處具有強吸光度的著色產物。該測定法可以在各種樣品中檢測<0.5mU的ALDH活性(單位定義見操作手冊)。
Cat # +Size | K731-100 |
Size | 100 assays |
Detection Method | Absorbance (450 nm) |
Species Reactivity | Mammalian |
Applications | The assay can detect < 0.5 mU of ALDH activity (based on our unit definition) in a variety of samples. |
Features & Benefits | • Simple procedure • Fast and convenient • Sensitive assay for measuring ALDH activity in various biological samples and the kit is also suitable for high throughput analyses. |
Kit Components | • ALDH Assay Buffer • Acetaldehyde • ALDH Substrate Mix (Lyophilized) • ALDH Positive Control (Lyophilized) • NADH Standard (0.5 µmol, Lyophilized) |
引用文獻
Choi NE et al., (2017) Effects of Cudrania Tricuspidata Root Extract (CTE) on Ethanol-Induced Hangover via Modulating Alcohol Metabolizing Enzyme Activities and Blood Gas Levels in Rats, Journal of the Korea Academia-Industrial cooperation Society, 2017, 18(2):218-225