Protein A (Lyophilized) 貨號6500B-10

Genetically engineered protein ideal for purification of polyclonal or monoclonal IgG antibodies

Protein A是一種經遺傳工程改造的蛋白質，其在N末端含有蛋白質A和6x His標籤的5個Ig結合區。 細胞壁結合區，白蛋白結合區和其他非特異性結合區已從重組蛋白A中消除，以確保最大特異性IgG結合。 6x His標籤可用於親和純化或使用抗His標籤抗體進行蛋白A檢測。Protein A是純化多克隆或單克隆IgG抗體的理想選擇。 Protein A與大多數人和小鼠IgG亞類（例如人IgG1，IgG2，IgG4;小鼠IgG1，IgG2a，IgG2b，IgG3）結合。 它還結合來自牛，豚鼠，倉鼠，馬，豬和兔的總IgG。 蛋白A對雞，山羊，大鼠和綿羊幾乎沒有親和力。

The recombinant Protein A is a genetically engineered protein containing five Ig-binding regions of protein A and 6x His-tag on N-terminus. Cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from the recombinant Protein A to ensure the maximum specific IgG binding. The 6x His-tag can be used for affinity purification or for protein A detection using anti-His-tag antibody. The recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to most human and mouse IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG1, IgG2a, IgG2b, IgG3). It also binds to total IgG from cow, guinea pig, hamster, horse, pig, and rabbit. Protein A has little affinity to chicken, goat, rat and sheep.

Alternate Name	Protein A
Gene ID	3919448
Accession #	YP_498670
Source	E. coli
Appearance	Lyophilized
Physical Form Description	Lyophilized powder
Molecular Weight	35.5 kDa
Purity by SDS-PAGE	≥97%
Purity by	≥97%
Reconstitution Instructions	Reconstitute in H₂O
Handling	Centrifuge the vial prior to opening.
Storage Conditions	-20°C
Shipping Conditions	Gel Pack
USAGE	For Research Use Only! Not to be used in humans

 引用文獻

Nagatsuka, Takehiro et al. (2017) Preparation of silicon nitride biochips for reflectometric interference spectroscopic (RIfS) analysis of biological toxins and E. coli O157:H7 strain, Sensors and Actuators B: Chemical, 2017 Jul;246:937-942