單胺氧化酶活性（總MAO / MAO-A / MAO-B）螢光測定試劑盒| 貨號K795 

Monoamine Oxidase Activity (Total MAO/MAO-A/MAO-B) Fluorometric Assay Kit

Monoamine oxidases單胺氧化酶（MAO，EC 1.4.3.4）是可以氧化多種endogenous primary amines的酶家族。由substrate，inhibitor specificity抑制劑特異性和組織定位鑑定了兩種同種型MAO-A和MAO-B。Clonogenic研究表明，這兩種同工酶具有相似的催化特性，但它們的氨基酸序列不同。 MAO-A有利於5-羥色胺，去甲腎上腺素和多巴胺作為底物，而phenylethylamine苯乙胺和benzylamine芐胺是MAO-B優選的底物。 MAO-A和MAO-B是線粒體結合的酶，其在整個腦和其他組織中普遍表達。 MAO水平的不平衡與精神分裂症，抑鬱症，注意力缺陷和其他疾病有關。 MAO-A與恐慌，焦慮和抑鬱有關，而MAO-B缺陷導致Alzheimer’s阿爾茨海默氏症和Parkinson’s diseases帕金森病。 BioVision的單胺氧化酶活性（總MAO / MAO-A / MAO-B）檢測是一種靈敏的檢測方法，可在Clorgyline和Selegiline特異性抑制劑存在下分別檢測總monoamine oxidase activity單胺氧化酶活性以及MAO-A和MAO-B isoenzyme活性。分別為MAO-A和MAO-B。該測定基於H 2 O 2的螢光檢測，H 2 O 2是MAO底物氧化脫氨過程中產生的副產物之一。該測定可以檢測少至5μU的MAO酶活性。

Cat # +Size	K795-100
Size	100 assays
Detection Method	Fluorescence (Ex/Em = 535/587 nm)
Applications	Measurement of Total MAO, MAO-A and MAO-B activities in various biological samples.
Features & Benefits	• Rapid & Convenient • The assay can detect as little as 5 µU of MAO enzymatic activity. • Includes MAO-A specific Inhibitor Clorgyline, MAO-B specific Inhibitor Selegiline & MAO-A Enzyme as Positive Control
Kit Components	• MAO Assay Buffer • OxiRed™ Probe (in DMSO) • MAO Substrate (Lyophilized) • Developer (Lyophilized) • Positive Control (MAO-A Enzyme) (Lyophilized) (Lyophilized) • MAO-A Inhibitor, Clorgyline (Lyophilized) • MAO-B Inhibitor, Selegiline (Lyophilized) • H2O2 Standard (0.88 M)

 

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