細胞凋亡系列商品- Annexin V-FITC Reagent | 貨號1001-200

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原廠連結：	http://www.biovision.com
相關下載：	Annexin V-FITC reagent操作手冊

特點

細胞凋亡系列商品– Annexin V-FITC Reagent | 貨號1001-200 (大量包裝) (buffer/PI需另購, 如下)

1X Binding 緩衝液, 100ml (1035-100)

Propidium Iodide 死細胞核酸染劑 | 貨號1056

Annexin V細胞染色，用於檢測細胞凋亡早期的細胞膜變化。在細胞凋亡早期，膜磷脂醯絲氨酸phosphatidylserine (PS)由脂膜內側翻向外側。Annexin V是一種Ca離子依賴性磷脂結合蛋白，可以與磷脂醯絲氨酸phosphatidylserine (PS) 特異結合。Annexin V結合不同染劑之標誌FITC、Cy3、EGFP、Cy5、PE、Biotin等；Annexin V通過流式細胞儀或螢光顯微鏡可以檢測到細胞凋亡的發生。結合細胞核染劑PI，利用流式細胞儀或螢光顯微鏡可偵測偵測細胞凋亡現象及其凋亡分期。碘化丙啶(Propidium Iodide, PI)是一種核酸染料，PI只能透過凋亡晚期和死細胞的細胞膜，因此Annexin V和PI結合使用，可以區分凋亡早晚期的細胞及死細胞。

細胞凋亡介紹: 細胞凋亡是指為維持有機體內環境穩定，由基因控制的細胞自主的有序的死亡。正常情況下，任何細胞在形成過程中發生的異常都會通過凋亡消除。例如，體內的癌細胞增長為腫瘤的過程會受細胞凋亡的引導而被抑制。然而，在抑癌基因p53出現問題時，凋亡就不會誘導發生，從而導致癌細胞的不斷增長。細胞凋亡可以通過細胞形態的變化或生物化學的變化來檢測。目前常用的指標有caspase活性分析、DNA片段分析、及磷脂醯絲氨酸phosphatidylserine (PS)的外翻等實驗。

在細胞凋亡過程中，磷脂酰絲氨酸 (PS) 從質膜的細胞質面轉移到細胞表面。 Annexin V 對 PS 具有很強的 Ca²ᶧ 依賴性親和力，因此可用作檢測細胞凋亡的探針。 Annexin V-FITC 偶聯物可用於通過螢光顯微鏡或流式細胞術檢測細胞凋亡。

Highlights	• Detection method- flow cytometry (Ex/Em = 488/530 nm) using FL1 channel and fluorescence microscopy • Sample- Cell culture • Formulation- 0.15 mg/ml in PBS containing 1% BSA, 0.03% Proclin • Simple, easy to use and convenient
Storage Conditions	4°C
Shipping Conditions	Gel Pack
USAGE	For Research Use Only! Not For Use in Humans.

引用文獻

Jun Chen, et al., ( 2018) An analysis of the expression and function of myeloid differentiation factor 88 in human osteosarcoma, Oncology Letters, Oct. 2018, 30250559

Crisan et al., Soluble uric acid primes TLR-induced proinflammatory cytokine production by human primary cells via inhibition of IL-1Ra. Ann Rheum Dis, Feb 2015; 10.1136/annrheumdis-2014-206564.

Shetty et al., Safety profile of accelerated corneal cross-linking versus conventional cross-linking: a comparative study on ex vivo-cultured limbal epithelial cells. Br J Ophthalmol, Feb 2015; 99: 272 – 280.

Cleophas et al., Suppression of monosodium urate crystal-induced cytokine production by butyrate is mediated by the inhibition of class I histone deacetylases. Ann Rheum Dis, Jan 2015; 10.1136/annrheumdis-2014-206258.

Herrmann et al., TLR9 Is Critical for Glioma Stem Cell Maintenance and Targeting. Cancer Res., Sep 2014; 74: 5218 – 5228.

Gaudette et al., Bcl-xL Protein Protects from C/EBP Homologous Protein (CHOP)-dependent Apoptosis during Plasma Cell Differentiation. J. Biol. Chem., Aug 2014; 289: 23629 – 23640.

更多Annexin V reagent商品

Catalog #	Product Name+	Size
1001-1000	Annexin V-FITC Reagent	1000 assays
1001-200	Annexin V-FITC Reagent	200 assays
1014-1000	Annexin V-PE Reagent	1000 assays
1014-200	Annexin V-PE Reagent	200 assays
1002-1000	Annexin V-Cy3 Reagent	1000 assays
1002-200	Annexin V-Cy3 Reagent	200 assays
1013-1000	Annexin V-Cy5 Reagent	1000 assays
1013-200	Annexin V-Cy5 Reagent	200 assays
1015-1000	Annexin V-PE-Cy5 Reagent	1000 assays
1015-200	Annexin V-PE-Cy5 Reagent	200 assays
1003-100	Annexin V-Biotin Reagent	100 µg
1003-1000	Annexin V-Biotin Reagent	1 mg
1004-1000	Annexin V-EGFP Reagent	1000 assays
1004-200	Annexin V-EGFP Reagent	200 assays
1056-1	Propidium Iodide	1 ml
1006-100	10X Annexin V Binding Buffer	100 ml
1035-100	1X Annexin V Binding Buffer	100 ml

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