Caspase-3 Substrate DEVD-AFC | 貨號1007-200

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品項: Caspase-3 Substrate DEVD-AFC | 貨號1007-200
產品介紹: 即用型DEVD-AFC為caspase-3/CPP32 (Km = 9.7 µM)螢光Substrate,活化的Caspase-3為蛋白水解酶;能多特異切割受質Ac-DEVD-AMC上,於D(aspartate)和AMC之間,AMC游離後會發出螢光 (ex400/em505nm)。可使用螢光計或多盤式螢光reader。 或者使用手持式長UV燈使螢光從切割時的藍色到綠色的變化。產品規格: Ac-Asp-Glu-Val-Asp-AFC | 純度>98% by HPLC | 濃度In DMSO (1 mM) | 分子量C₃₀H₃₃F₃N₅O₁₃
Ready-to-use fluorometric substrate for caspase-3/CPP32 (Km = 9.7 µM) and related caspases that recognize the amino acid sequence DEVD. The sequence DEVD is based on caspase-3 cleavage site in poly (ADP-ribose) polymerase (PARP). CPP32 and related caspase activity can be quantified by fluorescent detection of free AFC after cleaved from the peptide substrate DEVD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or a multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp.
Alternate Name | Ac-DEVD-AFC, Caspase-3 Substrate Fluorogenic |
---|---|
Peptide Sequence | Ac-Asp-Glu-Val-Asp-AFC |
Appearance | Liquid |
Formulation | In DMSO (1 mM) |
CAS # | 1762-34-1 |
Molecular Formula | C₃₀H₃₃F₃N₅O₁₃ |
Molecular Weight | 729 |
Purity | >98% by HPLC |
Solubility | DMSO |
SMILES | CC(C)C(C(=O)NC(CC(=O)O)C(=O)NC1=CC2=C(C=C1)C(=CC(=O)O2)C(F)(F)F)NC(=O)C(CCC(=O)O)NC(=O)C(CC(=O)O)NC(=O)C |
InChi | InChI=1S/C30H34F3N5O13/c1-12(2)25(38-26(47)17(6-7-21(40)41)36-28(49)18(10-22(42)43)34-13(3)39)29(50)37-19(11-23(44)45)27(48)35-14-4-5-15-16(30(31,32)33)9-24(46)51-20(15)8-14/h4-5,8-9,12,17-19,25H,6-7,10-11H2,1-3H3,(H,34,39)(H,35,48)(H,36,49)(H,37,50)(H,38,47)(H,40,41)(H,42,43)(H,44,45)/t17-,18-,19-,25-/m0/s1 |
InChi Key | GZDRODOYEFEHGG-NUDCOPPTSA-N |
PubChem CID | 644234 |
MDL Number | MFCD01310970 |
Handling | Potect from light and moisture |
Storage Conditions | -20°C |
Shipping Conditions | Gel Pack |
USAGE | For Research Use Only! Not For Use in Humans. |
Caspase訊息傳遞簡介
當細胞接收凋亡訊號後,將不活化態的pro-caspase轉變為活化態caspase,引發caspase cascade造成細胞凋亡。主要經由Caspases 8、9、12調控細胞凋亡的外在路徑(extrinsic pathway)、內在(intrinsic pathway)、以及ER stress,最後造成Caspases 3的活化。Biovision提供完整Caspase相關分析套組、inhibitor、substrate等各類商品。Caspase 分析套組/Substrate: 從Caspase1-13、Pan Caspase皆能分析。分析套組部分有化學呈色反應及螢光可選擇;Substrate部分有化學呈色物質pNA(λ= 400 nm)與螢光呈色物質AFC(Ex/Em=400/505 nm)兩種可應用。Caspase Inhibitors: Caspase 抑制劑主要結合Caspase活化位,進而達到訊息抑制效果。fluoromethylketone(FMK)類的抑制劑,為主要研究抗細胞凋亡的工具之一。新一代Caspase抑制劑Q-(XXXX)-OPh,因其細胞毒性較低,且穩定性較FMK類抑制劑高,較適合在in vivo實驗中使用。

商品型號 | 產品名稱 | 包裝規格 |
Caspase螢光分析套組 | ||
K110-100 | Caspase-1 Fluorometric Assay | 100 assays |
K116-100 | Caspase-2 Fluorometric Assay Kit | 100 assays |
K105-100 | Caspase-3 Fluorometric Assay Kit | 100 assays |
K126-100 | Caspase-4 Fluorometric Assay Kit | 100 assays |
K122-100 | Caspase-5 Fluorometric Assay Kit | 100 assays |
K114-100 | Caspase-6 Fluorometric Assay Kit | 100 assays |
K112-100 | Caspase-8 Fluorometric Assay Kit | 100 assays |
K118-100 | Caspase-9 Fluorometric Assay Kit | 100 assays |
K124-100 | Caspase-10 Fluorometric Assay Kit | 100 assays |
K139-100 | Caspase-12 Fluorometric Assay Kit | 100 assays |
Caspase呈色分析套組 | ||
K111-100 | Caspase-1 Colorimetric Assay Kit | 100 assays |
K117-100 | Caspase-2 Colorimetric Assay Kit | 100 assays |
K106-100 | Caspase-3 Colorimetric Assay Kit | 100 assays |
K127-100 | Caspase-4 Colorimetric Assay Kit | 100 assays |
K123-100 | Caspase-5 Colorimetric Assay Kit | 100 assays |
K115-100 | Caspase-6 Colorimetric Assay Kit | 100 assays |
K113-100 | Caspase-8 Colorimetric Assay Kit | 100 assays |
K119-100 | Caspase-9 Colorimetric Assay Kit | 100 assays |
K125-100 | Caspase-10 Colorimetric Assay Kit | 100 assays |
Caspase FMK類抑制劑 | ||
1012-100 1012-20C |
Caspase-1 Inhibitor, Z-YVAD-FMK | 100 μl(2 mM) 20 μl(10 mM) |
1073-100 1073-20C |
Caspase-2 Inhibitor, Z-VDVAD-FMK | 100 μl(2 mM) 20 μl(10 mM) |
1009-100 1009-20C |
Caspase-3 Inhibitor, Z-DEVD-FMK | 100 μl(2 mM) 20 μl(10 mM) |
1108-100 1108-20C |
Caspase-4 Inhibitor, Z-LEVD-FMK | 100 μl(2 mM) 20 μl(10 mM) |
1100-100 1100-20C |
Caspase-5 Inhibitor, Z-WEHD-FMK | 100 μl(2 mM) 20 μl(10 mM) |
1011-100 1011-20C |
Caspase-6 Inhibitor, Z-VEID-FMK | 100 μl(2 mM) 20 μl(10 mM) |
1064-100 1064-20C |
Caspase-8 Inhibitor, Z-IETD-FMK | 100 μl(2 mM) 20 μl(10 mM) |
1074-100 1074-20C |
Caspase-9 Inhibitor, Z-LEHD-FMK | 100 μl(2 mM) 20 μl(10 mM) |
1112-100 1112-20C |
Caspase-10 Inhibitor, Z-AEVD-FMK | 100 μl(2 mM) 20 μl(10 mM) |
1079-100 1079-20C |
Caspase-12 Inhibitor, Z-ATAD-FMK | 100 μl(2 mM) 20 μl(10 mM) |
1115-100 1115-20C |
Caspase-13 Inhibitor, Z-LEE-FMK | 100 μl(2 mM) 20 μl(10 mM) |
1010-100 1010-20C |
Caspase-Family Inhibitor, Z-VAD-FMK | 100 μl(2 mM) 20 μl(10 mM) |
1120-20C | Caspase Inhibitor Boc-D-FMK | 20 μl(10 mM) |