葡萄糖攝取比色測定試劑盒 | 貨號K676-100
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特點
Glucose Uptake Colorimetric Assay Kit
產品描述：葡萄糖攝取是研究細胞信號傳導和葡萄糖代謝的重要生物學過程。 在許多用於測量葡萄糖攝取的不同方法中，2-脫氧葡萄糖（2-DG）由於其與葡萄糖的結構相似性而被廣泛使用。 與葡萄糖一樣，2-DG可被葡萄糖轉運蛋白吸收並代謝成2-DG-6-磷酸（2-DG6P）。 然而，2-DG6P不能進一步代謝，因此在細胞中積累。 累積的2-DG6P與細胞對2-DG（或葡萄糖）的攝取成正比。 在BioVision的葡萄糖攝取比色測定試劑盒中，2-DG6P被氧化產生NADPH，其可以通過酶循環擴增反應來確定。 這種易於使用的非放射性試劑盒非常靈敏，可以檢測低至10 pmol /孔的葡萄糖攝取量。
| Cat # +Size | K676-100 |
| Size | 100 assays |
| Detection Method | Absorbance (412 nm) |
| Species Reactivity | Mammalian |
| Applications | This easy to use non-radioactive kit is highly sensitive and can detect glucose uptake as low as 10 pmol/well. |
| Features & Benefits | • Simple procedure • Fast and convenient • The assay is easy, non-radioactive, and highly sensitive |
| Kit Components | • Extraction Buffer • Neutralization Buffer • 2-Deoxyglucose (2-DG, 10 mM) • Assay Buffer • Enzyme Mix (lyophilized) • Recycling Mix (lyophilized) • 2-DG6P Standard (lyophilized) • Glutathione Reductase (lyophilized) • Substrate-DTNB (lyophilized) |
引用文獻
1. Yunjie Xu, et al., (2018) ABT737 reverses cisplatin resistance by targeting glucose metabolism of human ovarian cancer cells, International Journal of Oncology, Jul. 2018, 30015875
2. Gu Z et al., (2017) NEK2 Promotes Aerobic Glycolysis in Multiple Myeloma Through Regulating Splicing of Pyruvate Kinase, J Hematol Oncol, 2017, 13;10(1):17
3. Sun, Xianghui et al. (2017) MicroRNA-143 suppresses oral squamous cell carcinoma (OSCC) cell growth, invasion and glucose metabolism through targeting Hexokinase2, Biosci Rep. 2017 Jun 8;37(3).
4. Ji, Shunrong et al. (2016) FBW7 (F-box and WD Repeat Domain-Containing 7) Negatively Regulates Glucose Metabolism by Targeting the c-Myc/TXNIP (Thioredoxin-Binding Protein) Axis in Pancreatic Cancer. Clin Cancer Res. 2016 Aug 1;22(15):3950-60.
5. Shi, Rong-Liang et al. (2016) Expression, clinical significance and mechanism of Slit2 in papillary thyroid cancer, Int J Oncol. 2016 May;48(5):2055-62.
