GSH/GSSG螢光分析套組 |Glutathione (GSH/GSSG/Total) 貨號K264-100

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相關下載：	GSH/GSSG螢光分析套組操作手冊

特點

GSH/GSSG螢光分析套組Glutathione (GSH/GSSG/Total) Fluorometric Assay Kit | 貨號K264-100

Biovision提供一系列氧化相關分析套組；其中GSH/GSSG螢光分析套組，以螢光Fluorescence (Ex/Em 340/420 nm)偵測；可用於細胞組織lysate、細胞培養液、尿液、血漿、或血清之液體型式的來源。操作時間大約60-120分鐘。靈敏度2-400 ng/µl，可測量GSH、GSSG、或total glutathione.

穀胱甘肽 (Glutathione ) 是主要的細胞內低分子量硫醇 ( thiol )，在組織和細胞中對抗氧化壓力 (oxidative stress) 的細胞防禦中，扮演重要角色。市售的穀胱甘肽檢測試劑盒例如DTNB-酶循環穀胱甘肽測定試劑盒(DTNB-enzyme cycling glutathione assay kit)或基於單氯聯苯的測定試劑盒(Monochlorobimane based assay kit)幾乎無法區分還原型穀胱甘肽（ reduced glutathione, GSH; FW：307）和氧化型穀胱甘肽（oxidized glutathione , GSSG; FW：612）。 BioVision的穀胱甘肽檢測試劑盒 (BioVision’s Glutathione Detection Kit , K264) 為單獨檢測GSH，GSSG和總穀胱甘肽 (total glutathione ) 。提供了一個獨特，及方便的檢測工具。在測定中，OPA與GSH（不是GSSG）反應，產生螢光，因此可以特異性地定量GSH。添加還原劑將GSSG轉化為GSH，因此可以測定（GSH + GSSG）。為了特異性地測量GSSG，加入GSH quacher以除去GSH，防止與OPA的反應（而GSSG不受影響）。然後加入還原劑以破壞過量的quacher並將GSSG轉化為GSH。因此，GSSG可以被特異性定量。該試劑盒提供了獨特的程序和緩衝液配方，以消除蛋白質硫醇之干擾，和穩定溶液中的GSH和GSSG。

GSH/GSSG螢光分析套組 | K264-100內容物
• Glutathione Assay Buffer
• PCA (Perchloric Acid, 6N)
• KOH (<6N)
• OPA Probe (o-phthalaldehyde )
• Reducing Agent Mix
• GSH Quencher
• GSH Standard (FW: 307)

GSH/GSSG螢光分析套組

Cat # +Size	K264-100
Size	100 assays
Detection Method	Fluorescence (Ex/Em 340/420 nm)
Species Reactivity	Mammalian
Applications	The kit provides unique formula and buffers and procedures for detecting the GSH, GSSG, and total glutathione individually
Features & Benefits	• Simple procedure; takes only ~1-2 hours • Fast and convenient • The assay is easy to perform and detects 2-400 ng/µl of GSH, GSSG or total glutathione.
Kit Components	• Glutathione Assay Buffer • PCA (Perchloric Acid, 6N) • KOH (<6N) • OPA Probe (o-phthalaldehyde ) • Reducing Agent Mix • GSH Quencher • GSH Standard (FW: 307)
Storage Conditions	-20°C
Shipping Conditions	Gel Pack

引用文獻

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Odkhuu et al., Involvement of redox balance in in vitro osteoclast formation of RAW 264.7 macrophage cells in response to LPS.Innate Immunity, Feb 2015; 21: 194 – 202.

Ishaq et al., Atmospheric gas plasma–induced ROS production activates TNF-ASK1 pathway for the induction of melanoma cancer cell apoptosis. Mol. Biol. Cell, May 2014; 25: 1523 – 1531.

Chowdhury et al., Disuccinyl Betulin Triggers Metacaspase-Dependent Endonuclease G-Mediated Cell Death in Unicellular Protozoan Parasite Leishmania donovani. Antimicrob. Agents Chemother., Apr 2014; 58: 2186 – 2201.

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