NO一氧化氮檢測試劑盒 | Nitric Oxide Assay Kit貨號ABCAM ab65328
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特點
Nitric Oxide一氧化氮分析套組 | 貨號ABCAM ab65328 (BIOVISION K262-200 ) 2 x 96 tests
靈敏度為1.0 nmole nitrite/well或者 10 µM.
一氧化氮分析套組 貨號K262-200 | 200個分析 Nitric Oxide Colorimetric Assay Kit 。產品描述: 一氧化氮（NO）在神經傳遞、血管調節、免疫反應、與細胞凋亡中扮演重要角色。NO迅速被氧化為亞硝酸鹽和硝酸鹽其用於定量NO的產生。BioVision的的一氧化氮呈色分析試劑盒，提供簡單兩步驟，準確測量硝酸鹽總/亞硝酸鹽的含量。第一步使用硝酸還原酶將硝酸鹽轉化為亞硝酸鹽。第二步使用格里斯試劑，亞硝酸鹽轉化為深紫色偶氮化合物。azochromophore的量準確地反映樣品一氧化氮含量。此套組靈敏度為1.0 nmole nitrite/well或者 10 µM.。
功能研究 – 一氧化氮檢測試劑盒（比色法）(ab65328)
使用 ab65328 測量的血液 NOx 濃度。應用含有 NO 的 NE 後的血液 NOx 濃度。與對照組相比，應用含有 NO 的 NE 後血液 NOx 濃度水平增加了 3 倍。
功能研究 – 一氧化氮比色測定試劑盒 (ab65328)
細胞裂解物中測量的亞硝酸鹽和硝酸鹽顯示每 10 6 個細胞的數量（微摩爾）
功能研究 – 一氧化氮比色測定試劑盒 (ab65328)
生物製品中測量的亞硝酸鹽和硝酸鹽顯示濃度（微摩爾）
功能研究 – 一氧化氮比色測定試劑盒 (ab65328)
標準曲線（比色法）：減去背景讀數的重複平均值 (+/-SD)
| Cat # +Size | ABCAM ab65328 (K262-200 ) |
|---|---|
| Size | 200 assays |
| Detection Method | Absorbance (540 nm) |
| Species Reactivity | Mammalian |
| Applications | BioVision’s Nitric Oxide Colorimetric Assay Kit provides an accurate, convenient measure of total nitrate/nitrite in a simple two-step process. |
| Features & Benefits | • Simple two-step procedure; takes only ~1-1.5 hours • Fast and convenient • BioVision’s Nitric Oxide Colorimetric Assay Kit provides an accurate, convenient measure of total nitrate/nitrite. |
| Kit Components | • Assay Buffer • Enzyme cofactor • Enhancer • Nitrate Reductase • Nitrate Standard • Nitrite Standard • Griess Reagent R1 • Griess Reagent R2 • Microtiter Plate • Plate Cover |
引用文獻
1. Jessica Leigh Ritter, et al., (2018) Neisseria gonorrhoeae–Induced Inflammatory Pyroptosis in Human Macrophages is Dependent on Intracellular Gonococci and Lipooligosaccharide, Journal of Cell Death, Jan. 2018, 29434478
2. Aalinkeel, Ravikumar et al. (2017) Galectin-1 Reduces Neuroinflammation via Modulation of Nitric Oxide-Arginase Signaling in HIV-1 Transfected Microglia: a Gold Nanoparticle-Galectin-1 “Nanoplex” a Possible Neurotherapeutic?, J Neuroimmune Pharmacol. 2017 Mar;12(1):133-151.
3. Onaolapo, Eun Hye et al. (2017) Exogenous daytime melatonin modulates response of adolescent mice in a repeated unpredictable stress paradigm, Naunyn Schmiedebergs Arch Pharmacol. 2017 Feb;390(2):149-161.
4. Onaolapo, Olakunle J. et al. (2017) l-Methionine and silymarin: A comparison of prophylactic protective capabilities in acetaminophen-induced injuries of the liver, kidney and cerebral cortex, Biomed Pharmacother. 2017 Jan;85:323-333.
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