ExKine™ Pro 動物培養細胞/組織總蛋白提取試劑盒貨號KTP3007

ExKine™ Pro Total Protein Extraction Kit (Protein yield: 2-8 mg/mL)

ExKine™ Pro 動物培養細胞/組織總蛋白提取試劑盒(ExKine™ Pro Total Protein Extraction Kit), 是新一代超快速蛋白提取工具; 更多和更多證據表明，最常用的RIPA緩衝液(RIPA buffer)可能會導致蛋白質隨機丟失，導致許多困難的數據這很難解釋。 該試劑盒採用離心柱提取技術(centrifugal column extraction technologyc)結合優化的裂解緩衝液(optimized lysis buffer)進行提取更快速有效地檢測總蛋白(total protein)，使 WB 結果更準確。 提取系統可低至 20 μL採用離心柱提取蛋白質，有效解決樣品量少的問題。 該套件適用於SDS-PAGE、WB 和 IP 等應用。可用於裂解哺乳動物(mammalian adherent cells)貼壁細胞、非貼壁細胞和組織的快速高效解決方案組成，適用於許多不同的應用，例如報告基因檢測(reporter assays)、SDS-PAGE、蛋白質純化(protein purification)、Western 印跡(Western blot)，免疫沉澱(immunoprecipitation)。Protein yield: 2-8 mg/mL

實驗結果

更高的裂解效率 (More Lysis efficiency)

使用 D 製造商和 Abbkine 的 RIPA 緩衝液裂解分離的 293 細胞懸浮液。將細胞裂解物在 12000 g 下離心 10 分鐘。 1 顯示來自D 製造商的RIPA 裂解緩衝液（弱），2 顯示來自D 製造商的RIPA 裂解緩衝液（中），3 顯示來自D 製造商的RIPA 裂解緩衝液（強），4 顯示來自Abbkine 的RIPA 裂解緩衝液。 D 製造商的 RIPA 緩衝液顯示不完全裂解，透過管底部有明顯的不溶性顆粒證明，而 Abbkine 的 RIPA 裂解緩衝液顯示完全裂解。Isolated 293 cells suspension was lysed by RIPA buffer from D manufacturer and Abbkine. The cell lysates were centrifuged at 12000 g for 10 min. 1 shows RIPA Lysis Buffer(weak) from D manufacturer,2 shows RIPA Lysis Buffer(medium) from D manufacturer, 3 shows RIPA Lysis Buffer(Strong) from D manufacturer, 4 shows RIPA Lysis Buffer from Abbkine. RIPA buffer from D manufacturer show incomplete lysis as evidenced by the presence of obvious insoluble pellet at the bottom of the tube while RIPA Lysis Buffer from Abbkine shows complete lysis.

高蛋白產量(High protein yield) 

採用不同國際品牌、國內知名品牌的蛋白萃取試劑盒產品對小鼠肝組織進行蛋白萃取實驗，包括變性裂解液RIPA和非變性裂解液的處理。結果如下：Different international brands and well-known domestic brands of protein extraction kit products were used to perform protein extraction experiments on mouse liver tissue, including the treatment of denaturated lysate RIPA and non-denaturated lysate. The results are as follows:

In the results of protein extraction by column method of Abbkine, the protein concentration obtained by denatured cleavage was as high as 20.06 mg/mL, and the protein concentration obtained by non-denatured cleavage was 20.99 mg/mL, which ranked top among many products.

No protein loss

Protein profiles of mouse liver extracted by denatureing lysis buffer and native buffer from different manufacturers. Extracted proteins were separated in 10% SDS-PAGE and stained with coomassie blue. Lane 1, Marker; Lane 2, native buffer from A manufacturer; Lane 3, native buffer from Abbkine; Lane 4, denatureing lysis buffer from A manufacturer; Lane 5, denatureing lysis buffer from B manufacturer; Lane 6, denatureing lysis buffer from C manufacturer; Lane 7, denatureing lysis buffer from Abbkine; Lane 8, denatureing lysis buffer from D manufacturer.

 

Nuclear protein and membrane protein can be extracted

Total proteins were extracted from 293 cells and mouse liver. Lane 1, 5 μL proteins extracted by assay kits of B manufacturer; Lane 2, 2.5 μL proteins extracted by assay kits of B manufacturer; Lane 3, 5 μL proteins extracted by Abbkine assay kits; Lane 4, 2.5 μL proteins extracted by Abbkine assay kits.

The results showed that the Abbkine kit could extract nuclear protein and membrane protein more particularly effective, and the protein bands with clear background could be obtained in both cells and tissues.

產品貨號	品項	反應	測量儀器
貨號KTA1001	活細胞和死細胞雙染色試劑盒	100個反應	活細胞綠色螢光染劑Ex/Em = 488/530 nm; 死細胞紅色螢光染料(Ex/Em = 535/617)
貨號 KTA2010	TUNEL細胞凋亡檢測試劑盒	50個反應	螢光酶標儀、螢光顯微鏡或流式細胞儀。FITC通道（Ex / Em = 490nm / 520 nm）
貨號 KTC011001	CCK-8細胞增殖和細胞毒性試劑盒	500個反應	吸收光450nm
貨號KTC4003	TraKine™粒線體染色	50個反應/250個反應	Ex / Em = 490/523 nm增殖(proliferating)和非增殖細胞(non-proliferating cells)
貨號KTC4100	Pro活細胞微管蛋白染色	50個反應/250個反應	Ex/Em = 500/520 nm
貨號KTC4001	Abbkine 細胞膜染色	100個反應/500個反應	Ex/Em = 484/501 nm
貨號BMD00063	DAPI Staining Solution	10MG/100MG	Ex/Em: 358/461 nm (with DNA)
貨號KTC4210	活細胞Lysosome染色	50個反應/250個反應	deep red fluorescent dye , Ex/Em:650/665nm
貨號KTA3030	細胞衰老檢測試劑盒（β-半乳糖苷酶）	100個反應	光學顯微鏡
貨號KTA1020	CCK-8細胞增殖和細胞毒性試劑盒	1000個反應/10,000個反應	吸收光450nm
貨號KTA2030	細胞增殖EdU Image試劑盒 (BrdU替代法)	100個反應/500個反應	AbFluor 488 azide (Ex/Em = 501/525 nm)
貨號KTB1400	一氧化Dan（NO）檢測試劑盒	96個反應/ 192個反應	colorimetric (540 nm) 檢測範圍：1-100 µM
貨號KTB1070	CheKine™黃嘌呤氧化酶檢測	96個反應/ 480個反應	OD 450 nm 線性範圍15.6 -500 mU/
貨號KTD3001	BCA蛋白定量試劑盒	2000個反應/ 5000個反應	OD 562 nm 相容性 5 % SDS, 5 % Triton X-100, 5 % Tween-20, 60, 80 50 to 1000 µg/ml.
貨號KTB1110	乳酸脫氫酶（LDH）測定試劑盒	96個反應/ 480個反應	λmax= 450 nm 1- 20 U/mL of LDH
貨號PRP100153	IGF-I重組蛋白	200UG/1MG	human IGF1 isoform 1 (P05019-1) (Gly 49-Ala 118) > 95 % as determined by SDS-PAGE
貨號 KTA2020	細胞週期染色檢測	100個反應/500個反應	Ex/Em=535/615 nm.
貨號BMC21400	PurKine™內毒素去除預裝離心柱	1ml×5 3mlx5	More than 2,000,000 EU/mL to eliminate >99% of endotoxins.
貨號KTA1200	CheKine™蛋白羰基比色測定試劑盒	48個反應/ 96個反應	吸收光370 nm
貨號KTB1080	超氧陰離子清除測定試劑盒	48個反應/ 96個反應	Superoxide anion scavenging rate D% =（∆∆ODControl – ∆∆ODSample）÷ ∆∆ODControl ×100%
貨號KTB1090	清除氫氧自由基能力測定試劑	48個反應/ 96個反應	吸收光 536nm Hydroxyl radical scavenging rate D% =（ASample – AControl）÷（ABlank – AControl）×100%
貨號KTB2220	TC總膽固醇檢測試劑盒	48個反應/ 96個反應	500nm Standard: 5 μmol/mL
貨號KTB1510	CheKine™尿酸（UA）比色分析試劑盒	48個反應/ 96個反應	吸收光505 nm Standard: 5 μmol/mL.
貨號KTB1300	Glucose葡萄糖測定試劑盒	96個反應/ 192個反應	吸光值630nm 4- 300 mg/dL)
貨號KTB1100	Lactate  乳酸檢測試劑盒	96個反應/ 480個反應	colorimetric (450 nm) 0.03 mM-2 mM
貨號KTB1700	組織和血液鹼性磷酸酶（ Alkaline Phosphatase, AKP / ALP）比色測定	96個反應/ 96 X5盤	吸收光510 nm
貨號KTB1500	總抗氧化劑能力TAC分析	96個反應/ 480個反應	593 nm 偵測範圍: 0.15-3 mM
貨號KTA0002	Annexin V-AbFluor™488細胞凋亡檢測試劑盒	20個反應/50個反應/100個反應	Annexin V- AbFluor™ 488: Abs/Em = 491/517 nm; PI: Abs/Em = 535/617 nm (with DNA)
貨號KET7013	EliKine™ Rat IL-6 ELISA Kit試劑盒	96個反應/ 96 X5盤	450 nm 偵測範圍: 250pg/mL-16000 pg/mL, 最低靈敏度100 pg/mL
貨號KET0003	雌二醇（Estradiol , E2）試劑盒	96個反應/ 96 X5盤	450 nm 偵測範圍40 pg/ml-1500 pg/ml
貨號KET6032	TNF–α Alpha型腫瘤壞死因子 ELISA試劑盒	96個反應/ 96 X5盤	450 nm 7.8 pg/ml-500 pg/ml
貨號KET6033	VEGF血管內皮生長因子ELISA試劑盒	96個反應/ 96 X5盤	450 nm 偵測範圍: 31.25 pg/mL-2000 pg/mL
貨號KET7005	白介素-1β（Interleukin-1β, IL-1β）ELISA試劑盒	96個反應/ 96 X5盤	Microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm. 偵測範圍: 15.6 pg/ml-1000 pg/ml
貨號KTE70003	小鼠黃嘌呤脫氫酶/氧化酶（XDH）ELISA試劑盒	96個反應/ 96 X5盤	450 nm 偵測範圍: 22.5 ng/L-360 ng/L, 最低靈敏度1.0 ng/L。
貨號 A02051HRP	His-tag標籤多株抗體-HRP	50ul/200ul	WB (1:5000).
貨號A02170MGB	Anti-V5 Tag Mouse Monoclonal Antibody (11D5), 磁珠	100ul/400ul/2ml	GKPIPNPLLGLDST epitope
貨號KTA4001	JC-1線粒體膜電位測定試劑盒	100個反應/ 500個反應	（聚結的JC-1，Ex / Em = 585/590 nm）發出綠色螢光（JC-1單體，Ex / Em = 510/527 nm） JC-1 Stain CCCP (10 mM)  Assay Buffer (5×)
貨號KTB1600	穀胱甘肽(Glutathione)檢測	96個反應	412 nm 2-200 µg/mL
貨號BMD00063	DAPI Staining Solution	1MG/10MG/100MG	λEx/λEm: 358/461 nm (with DNA)
貨號KTP3001	細胞核/細胞質分離試劑盒	50個反應/ 200個反應	Cytoplasmic Solution A (CES A) Cytoplasmic Solution B (CES B) • Nuclear Extraction Solution (NES) DTT (500X) Protease Inhibitor (100X)
貨號BMD00064-50UG	Calcein AM鈣黃綠素活細胞螢光染劑	50ug/1mg/10mg	λEX/λEm: 494/517 nm (pH 8) (calcein)
貨號 KTB1660	硫氧還蛋白過氧化物酶（TPX）檢測	96個反應	240nm
貨號KTB1020	NAD/NADH 分析試劑盒	96個反應/ 480個反應	OD565 nm 96 孔板測定中的檢測限值為 0.78 μM 線性最高 50 μM NAD+/NADH。
貨號KTB1070	CheKine™黃嘌呤氧化酶檢測	96個反應/ 480個反應	OD 450 nm 線性範圍15.6 -500 mU/mL
貨號KTB1040	Catalase過氧化氫酶活性檢測試劑盒	96個反應/ 480個反應	OD 540 nm 2-75 µM
貨號KTB1060	過氧化物酶（Peroxidases）分析試劑盒	96個反應	OD 664 nm 線性範圍為15.6-1000 µU/mL
貨號KTB1050	脂質過氧化檢測試劑盒(MDA)	96個反應	OD 532 nm 1-100 µM/L
貨號KTB1030	超氧化物歧化酶（SOD）活性測定試劑盒	96個反應	200-3.13 U/ml
貨號KTA1030	LDH細胞毒性測定試劑盒	96個反應/ 480個反應	OD 565 nm 10,00-100,0000 cells per well