白介素-1β（Interleukin-1β, IL-1β）ELISA試劑盒 | 貨號KTE7005 15.6 pg/ml-1000 pg/ml, 最低靈敏度8 pg/mL

白介素-1β（Interleukin-1β, IL-1β）ELISA試劑盒採用兩點夾心ELISA(Sandwich ELISA)定量樣品中的白介素-1β（Interleukin-1β, IL-1β）。對白介素-1β（Interleukin-1β, IL-1β）特異的抗體已預先包被在微孔板上。將標準品和样品吸取到孔中，並且存在的任何白介素-1β（Interleukin-1β, IL-1β）都被固定的抗體結合。除去所有未結合的物質後，將HRP-綴合白介素-1β（Interleukin-1β, IL-1β）檢測抗體添加到孔中。洗滌以除去任何未結合的HRP試劑後，將色原溶液添加到孔中，並且根據在初始步驟中結合的白介素-1β（Interleukin-1β, IL-1β）的量成比例地顯色。停止顯色並測量顏色的強度。偵測範圍: 15.6 pg/ml-1000 pg/ml, 最低靈敏度8 pg/mL。可應用於以下樣品來源: Cell culture supernatants, Other biological fluids, Plasma, Serum。

     

細胞因子(Cytokine)功能

細胞因子(Cytokine)具有多種生物學功能。它是急性期反應(acute phase response)的有效誘導劑。在B細胞(B-cells)最終分化為Ig分泌細胞(Ig-secreting cells)的最終分化中起重要作用，參與淋巴細胞(lymphocyte)和單核細胞(monocyte)的分化。它誘導骨髓瘤(myeloma)和漿細胞瘤(plasmacytoma growth)生長，並誘導神經細胞分化(differentiation)。作用於B細胞(B-cells)，T細胞(T-cells)，肝細胞(hepatocytes)，造血祖細胞(hematopoeitic progenitor cells)和CNS中樞神經系統細胞。也可以作為肌肉因子(myokine)。它在肌肉收縮後排入血液，並起到增加脂肪分解和改善胰島素抵抗(insulin resistance)的作用。

白介素-1（Interleukin-1, IL-1）是有史以來最早的細胞因子(cytokines)之一。它的最初發現是可能誘發發燒(fever)，控制淋巴細胞(lymphocytes)，增加骨髓細胞數量(bone marrow cells)並引起骨關節退化的因素。此時，IL-1以其他幾種名稱為人所知，包括內源性熱原(endogenous pyrogen)，淋巴細胞活化因子(lymphocyte activating factor)，造血素-1(haemopoetin-1)和單核細胞因子(mononuclear cell factor)等。大約在1984年至1985年間，科學家們證實IL-1實際上由兩種不同的蛋白質組成，現在稱為IL-1α和IL-1β，它們屬於稱為白細胞介素1超家族的細胞因子家族。 IL-1β由巨噬細胞(macrophages)，單核細胞(monocytes)和樹突狀細胞(dendritic cells)產生。

Fig.1. Mouse IL-1β Standard Curve.

ELISA儀器設備請按此  (標配405、450、492、630 nm濾片/最高可擴充至7濾片）（KTE7005  absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.λ= 570 nm）

Product name	EliKine™ Mouse IL-1β ELISA Kit
Reactivity	Mouse
Applications	ELISA
Applications notes	EliKine™ Mouse IL-1β ELISA Kit employs a two-site sandwich ELISA to quantitate IL-1β in samples. An antibody specific for IL-1β has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-1β present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-1β is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-1β bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method	Colorimetric
SampleType	Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type	Sandwich ELISA (quantitative)
Assay duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative	IL1B; IL-1; IL1-BETA; IL1F2; catabolin; preinterleukin 1 beta; pro-interleukin-1-beta
Kit components	• Mouse IL-1β microplate • Mouse IL-1β standard • Mouse IL-1β detect antibody • EliKine™ Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers.
Features & Benefits	EliKine™ Mouse IL-1β ELISA Kit has high sensitivity and excellent specificity for detection of Mouse IL-1β. No significant cross-reactivity or interference between Mouse IL-1β and analogues was observed.
Calibration range	15.6 pg/ml-1000 pg/ml
Limit of detection	8 pg/mL
Usage notes	• Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions	The unopened kit should be stored at 2 – 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Interleukin-1 (IL-1) is one of the first cytokines ever described. Its initial discovery was as a factor that could induce fever, control lymphocytes, increase the number of bone marrow cells and cause degeneration of bone joints. At this time, IL-1 was known under several other names including endogenous pyrogen, lymphocyte activating factor, haemopoetin-1 and mononuclear cell factor, amongst others. It was around 1984-1985 when scientists confirmed that IL-1 was actually composed of two distinct proteins, now called IL-1α and IL-1β.These belong to a family of cytokines known as the interleukin-1 superfamily.Both IL-1α and IL-1β are produced by macrophages, monocytes and dendritic cells.
Gene ID	16176
Alternative	IL1B; IL-1; IL1-BETA; IL1F2; catabolin; preinterleukin 1 beta; pro-interleukin-1-beta
Accession	P10749

 

使用注意事項

• 請勿混用不同試劑盒中的組分，或在試劑盒到期日期之後使用試劑。
• 打開之前，讓所有試劑至少加室溫30分鐘。
• 用試劑預清洗移液器吸頭，為每個樣品，標準液和試劑使用新鮮的移液器吸頭，以免污染。
• 未使用的孔必須在提供的密封袋中於4°C乾燥。
• 徹底混合對於結果非常重要。建議每10分鐘使用低頻振盪器或稍稍搖動手。
• 建議所有樣品和標準品一式兩份或一式三份進行測定。

引用文獻

Evaluation of phage therapy in the treatment of Staphylococcus aureus-induced mastitis in mice

Huijun Geng, Wei Zou, Meixia Zhang, et al. Folia Microbiol (Praha). 2019 Jun 30

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