His-tag標籤多株抗體  | 貨號A02051

His; 6 His epitope; Hexa His; HHHHHH epitope; Polyhistidine Tag

His-tag標籤介紹: His-tag多組氨酸標籤(polyhistidine-tag)是蛋白質中的氨基酸基序(histidine (His) residues)，通常由蛋白質的N或C端組成，由至少五個組氨酸（His）殘基組成。它也被稱為六組氨酸標籤(6xHis-tag)，6xHis標籤和商標名稱His-tag。多組氨酸標籤(Polyhistidine-tags)通常用於親和純化在大腸桿菌(Escherichia coli)和其他原核表達系統(prokaryotic expression)中表達的多組氨酸標籤的重組蛋白(recombinant proteins)。A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

Fig. Western blot analysis of His fusion protein in cell lysate from transfected 293T cells (lane A) and baculovirus expression vector system (lane B) at 1:2000 dilutions.

產品描述： His-tag標籤多株抗體。Abbkine提供一系列Tag單多株抗體，抗His標籤兔多株抗體，免疫原為合成胜肽(Synthetic Peptide)。經由Rabbit免疫後, 抗體純化並保存於Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol. 抗His標籤兔多株抗體可應用於Western blot實驗。

活細胞和死細胞雙染色套組  |  CCK-8細胞增殖和細胞毒性試劑盒  | LDH細胞毒性測定試劑盒 | 細胞衰老檢測試劑盒（β-半乳糖苷酶 | 細胞增殖EdU Image試劑盒 

 

• Product name

Anti-His Tag Rabbit Polyclonal Antibody

• Immunogen

Synthetic Peptide

• Host

Rabbit

• Applications

WB

• Application notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000).

• Clonality

Polyclonal

• Isotype

Rabbit IgG

• Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen

• Formulation

Liquid solution

• Storage buffer

Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.

• Storage instructions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

• Shipping

Gel pack with blue ice.

 

貨號	產品名稱	胞器染色位置	螢光波段
貨號KTC4003	TraKine™粒線體染色	粒線體(mitochondria)	Ex / Em = 490/523 nm	增殖(proliferating)和非增殖細胞(non-proliferating cells)
貨號KTC4100	Pro活細胞微管蛋白染色	微管蛋白(Tubulin)	Ex/Em = 500/520 nm	活細胞或固定之細胞
貨號KTC4001	Abbkine 細胞膜染色	細胞膜 (plasma membrane)	Ex/Em = 484/501 nm	活細胞、固定懸浮、貼附細胞
貨號BMD00063	DAPI Staining Solution	細胞核 dsDNA	Ex/Em: 358/461 nm (with DNA)	染色活細胞和固定細胞
貨號KTC4210	活細胞Lysosome染色	Lysosome	deep red fluorescent dye , Ex/Em:650/665nm	染色活細胞和固定細胞