POD過氧化酶（Peroxidases）分析試劑盒| 貨號KTB1150

CheKine™ Micro Peroxidase (POD) Activity Assay Kit

POD過氧化酶(Peroxidase)廣泛存在於植物體內的不同組織，屬活性較高的誘導酶，能夠反應植物的代謝狀況和對環境的適應性。 POD 過氧化酶(Peroxidase)具有雙重的作用特性，在逆境或衰老初期的表現為清除 H₂O₂ ，達到保護細胞的功能。但在逆境或衰老後期，則參與活性氧的生成、葉綠素的降解，引發膜脂過氧化作用，加速細胞的衰老死亡，成為植物的衰老指標。植物體清除活性氧的保護系統又稱為抗氧化系統， 包括抗氧化酵素及非酵素型抗氧化 劑 （表 2 ），以應付具擴散性、水溶性及可與各種生物分子反應之活性氧化物，避免發生使活性物種毒性加強的連鎖反應。(出自農業部植物對環境逆境之調控與應用100年9月(第231期)。

過氧化酶（Peroxidases , EC編號1.11.1.x）是一大類酶，通常催化以下形式的反應：ROOR’+電子給體（2 e-）+ 2H +→ROH + R’OH。對於這些酶中的許多酶，最佳底物是過氧化氫(hydrogen peroxide)，但其他酶則對有機氫過氧化物(organic hydroperoxides)（例如脂質過氧化物, lipid peroxides）更具活性。過氧化物酶(Peroxidases)可在其活性位點(active sites)包含血紅素輔因子(heme cofactor)，或在氧化還原活性的半胱氨酸(redox-active cysteine)或硒代半胱氨酸殘基(selenocysteine residues)上具有替代性。過氧化物酶（Peroxidase ）偶聯物被廣泛用作ELISA，免疫組織化學技術以及Northern，Southern和Western印跡分析的輔助檢測試劑。過氧化物酶，POD（EC 1.11.1.7）廣泛存在於動物、植物、微生物和培養細胞中。能催化過氧化氫(hydrogen peroxide)、酚類( phenols )和胺類化合物(amine compounds)的氧化，並消除過氧化氫(hydrogen peroxide)、酚類(phenols )和胺類(amines)。毒性的雙重作用。CheKine™ Micro過氧化酶分析試劑盒，該試劑盒提供了一種檢測植物組織(plant tissues)、細菌(bacteria)、真菌(fungus )或細胞等多種生物樣本中 POD 活性的簡單方法。在測定中，POD透過H2O2催化特定底物的氧化，產生在460nm處具有特徵光吸收的有色物質。

Product name	CheKine™ Micro Peroxidase (POD) Activity Assay Kit
Applications notes	The kit provides a simple method for detecting POD activity in a variety of biological samples such as plant tissues,bacteria, fungus or cells . In the assay,POD catalyzes the oxidation of specific substrates by H2O2 to produce colored substances with characteristic light absorption at 460nm.
Alternative	peroxide reductases
Kit components	• Extraction Buffer • Substrate • H2O2
Features & Benefits	• Determination of POD activity in a variety of biological samples such as plant tissues,bacteria, fungus or cells . • Detailed sample preparation and result calculation methods are provided. • Simple operation steps.
Usage notes	• Do not mix or substitute reagents or materials from other kit lots or vendors. • Avoid foaming or bubbles when mixing or reconstituting components. • Avoid cross-contamination, change pipette tips between additions of standards, samples and reagents. Also, use separate reservoirs for each reagent. • Ensure all reagents and equipment are at the appropriate temperature before starting the assay.
Storage instructions	Storage at 4°C and protected from light upon receipt. Kit has a storage time of 12 months form receipt.
Shipping	Gel pack with blue ice.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Peroxidase, POD (EC 1.11.1.7) is widely present in animals, plants, microorganisms and cultured cells. It can catalyze hydrogen peroxide, oxidation of phenols and amine compounds, and eliminate hydrogen peroxide, phenols and amines. The dual effect of toxicity.
Alternative	peroxide reductases

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