TraKine™Pro活細胞微管蛋白染色 | Tubulin Staining Kit貨號KTC4100
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TraKine™Pro活細胞微管蛋白染色 | 貨號KTC4100
微管蛋白(Tubulin)是微管(microtubules.)的主要構建基塊。這種胞內圓柱形絲狀結構(cylindrical filamentous structure)幾乎存在於所有真核細胞(eukaryotic cells.)中。微管(Microtubules)在有絲分裂(mitosis)，細胞內運輸(intracellular transport)，鞭毛運動(flagellar movement)和細胞骨架(cytoskeleton.)中充當結構和移動元件。微管蛋白(Tubulin)是異二聚體(heterodimer)，由α-微管蛋白(a-tubulin)和b-微管蛋白(b-tubulin)組成。兩個亞基的分子量均為55 kDa，並具有相當的同源性(homology)。研究最多的微管蛋白(tubulins)已從脊椎動物腦(vertebrate brains)中分離出來。可以在免疫螢光顯微鏡下觀察微管(microtubules)，這使得能夠觀察超分子結構形式的蛋白質的細胞內組織。Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton. Tubulin is a heterodimer, which consists of a-tubulin and b-tubulin; both subunits have a molecular weight of 55 kDa and share considerable homology. The most studied tubulins have been isolated from vertebrate brains. The microtubules can be viewed in immunofluorescent microscopy, which enables the observation of the intracellular organization of proteins that are in the form of a supramolecular structure.
產品描述：
TraKine™Pro是一系列長效型細胞影像染劑，可標記活細胞或固定之細胞的細胞內結構。 TraKine™Pro專有的優異螢光染料，其螢光範圍由UV可、見光至近IR光譜。另提供基於TraKine™Pro技術的客製化產品。 TraKine™Pro活細胞微管蛋白染色試劑盒（TraKine™ Pro Live-cell Tubulin Staining kit, KTC4100）(超高分辨率的綠色螢光, Ex / Em = 500/520 nm）是一種螢光成像工具，用於對哺乳動物活細胞中微管蛋白進行高特異性和低背景染色。探針為不透膜，但與buffer T incubation後，它可以通過囊泡區室(vesicular compartments)的內吞途徑(endocytosis pathway)進入細胞，隨後釋放到細胞質(cytoplasm)中。它特別適合於長期超分辨率成像。
- Kit components• TubGreen™ (200 uM)
• Buffer T - Features & Benefits• Optimized staining protocol for labeling Tubulin in mammalian living cells.
• Especially suitable for Confocal and long-term super-resolution imaging (such as SIM, STED, TIRF, STORM and PALM).
• Proprietary TubGreen™ (Ex/Em = 500/520 nm)-high specificity, low background and excellent photostability.
• Low levels of cytotoxicity. - Usage notesMake sure the pipette tips and PCR tubes were sterilized at high temperature and pressure. Make sure sterile environment and protect from light during the whole experiment.
- Storage instructionsRefer to list of materials supplied for storage conditions of individual components. Stable for at least 6 months at recommended temperature from date of shipment.
- ShippingGel pack with blue ice.
Fig.1. SIM imaging results of U2OS cells using Abbkine TraKine™ Pro Live-cell Tubulin Staining kit (Green Fluorescence with Super Resolution). It can be seen that the kit can specifically label microtubule structures in living cells and perform ultra-high resolution imaging.
Fig.2. SIM imaging results of U2OS cells using Abbkine TraKine™ Pro Live-cell Tubulin Staining kit (Green Fluorescence with Super Resolution). It can be seen that the kit can specifically label microtubule structures in living cells and perform ultra-high resolution imaging.
Fig.3. Confocal imaging results of U2OS cells using Abbkine TraKine™ Pro Live-cell Tubulin Staining kit (Green Fluorescence with Super Resolution). It shows that the kit has high labeling efficiency.
Fig.4. Confocal imaging results of U2OS cells using Abbkine TraKine™ Pro Live-cell Tubulin Staining kit (Green Fluorescence with Super Resolution). It shows that the kit has high labeling efficiency.
引用文獻
L Zhao, Y Jia, C Zhao. Acta biomaterialia, 2019 – Elsevier
Shuangshuang Wang, Jixuan Song, Yuchao Li, et al. Reactive and Functional Polymers. July 2019, 140: 48-55.
| 貨號 | 產品名稱 | 胞器染色位置 | 螢光波段 | |
| 貨號KTC4003 | TraKine™粒線體染色 | 粒線體(mitochondria) | Ex / Em = 490/523 nm | 增殖(proliferating)和非增殖細胞(non-proliferating cells) |
| 貨號KTC4100 | Pro活細胞微管蛋白染色 | 微管蛋白(Tubulin) | Ex/Em = 500/520 nm | 活細胞或固定之細胞 |
| 貨號KTC4001 | Abbkine 細胞膜染色 |
(plasma membrane) |
Ex/Em = 484/501 nm | 活細胞、固定懸浮、貼附細胞 |
| 貨號BMD00063 | DAPI Staining Solution | 細胞核 dsDNA | Ex/Em: 358/461 nm (with DNA) | 染色活細胞和固定細胞 |
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