CheKine™黃嘌呤氧化酶檢測 | CheKine™ Xanthine Oxidase Assay Kit貨號KTB1070
| 代理廠牌： |  |
| 原廠連結： | |
| 相關下載： |
CheKine™黃嘌呤氧化酶檢測 | CheKine™ Xanthine Oxidase Assay Kit貨號KTB1070
黃嘌呤氧化酶（Xanthine oxidase , EC 1.17.3.2）或黃嘌呤氧化還原酶(xanthine oxidoreductase) 被認為是嘌呤(purine)在人體內分解代謝的末端酶，可催化次黃嘌呤(hypoxanthine)氧化為黃嘌呤(xanthine)然後變為尿酸(uric acid)。 當充當為NADH氧化酶 (NADH oxidase) 時，XO是超氧化物（superoxide , O2-）的生成器(generator)，超氧化物（superoxide , O2-）是一種強大的活性氧（reactive oxygen species , ROS）。 還已經發現XO在缺血再灌注損傷(ischemia-reperfusion injury)期間會產生過氧化氫（hydrogen peroxide , H2O2）和超氧化物（superoxide , O2-）。 由於它們的高反應性，這些ROS影響細胞的各種分子成分，過量會導致細胞變性(cell degeneration)和死亡(death)。 XO（Xanthine oxidase , EC 1.17.3.2）在健康個體的肝臟(liver)和空腸(jejunum)中存在大量。 但是，在各種肝臟疾病(liver disorders)中，已發現循環XO的水平急劇增加。 因此，血清XO(serum XO)水平的測定可作為急性肝損傷(acute liver damage), 如黃疸( jaundice）的敏感指標。
產品描述： CheKine™黃嘌呤氧化酶檢測試劑盒(CheKine™ Xanthine Oxidase Assay Kit, KTB1070) 提供了一種簡便的比色測定法，用於定量測定各種樣品中存在的黃嘌呤氧化酶 （Xanthine oxidase, XO）。 在測定中，黃嘌呤氧化酶 （Xanthine oxidase, XO）將黃嘌呤氧化成超氧化物（superoxide , O2-）。 超氧化物（superoxide , O2-）與四唑鹽WST-8 (tetrazolium salt WST-8) 染料反應形成水溶性的formazan product產物，可以很容易地在OD 450 nm處進行比色定量。 因此，樣品中存在的黃嘌呤氧化酶 （Xanthine oxidase, XO）與所獲得的訊號成比例。線性範圍15.6 -500 mU/mL.
引用文獻 Figure 2 Febuxostat relieves serum and renal tissue XO activity, and XO knockdown attenuates kidney injury in SI-AKI mice. (a, b) The XO activity in serum and renal tissue homogenates was measured 24 h after LPS injection. XO could oxidize xanthine and produce O2●¯, which could react with WST-8, and the color of the reaction products could be detected with a micro reader at 450 nm. (c, d) Changes in XO expression in the kidneys of SI-AKI mice were detected by immunofluorescence assay (400x magnification). (e) Timeline of AAV injection displayed. (f–i) Knockdown of XO expression was confirmed using western blot and immunofluorescence assays. (j, k) Serum and renal XO activity were evaluated. (l, m) BUN and Scr were analyzed after downregulation of XO and challenge with LPS (10 mg/kg). (n, o) Representative images of kidney tissue are presented, and the injury score was graded in a double-blinded manner. , , and vs. Ctrl; #, ##, and ### vs. LPS+Veh or LPS+Ctrl-shR (). Ctrl: control; Veh: vehicle; LPS: lipopolysaccharide; Feb: febuxostat; XO: xanthine oxidase; BUN: blood urea nitrogen; Scr: serum creatinine.
| Product name | CheKine™ Micro Xanthine Oxidase Activity Assay Kit |
| Applications notes | CheKine™ Micro Xanthine Oxidase Assay Kit provides a simple and easy colorimetric assay for the quantitative determination of the XO present in a variety of samples. In the assay, XO oxidizes xanthine to superoxide (O2-). Superoxide (O2-) reacts with a tetrazolium salt WST-8 dye to form a water-soluble colored formazan product, which can be easily quantified colorimetrically at OD 450 nm. Therefore, the Xanthine Oxidase present in the sample is proportional to the signal obtained. |
| Kit components | • Assay Buffer • Sample Diluent • WST-8 • Enhancer • Xanthine |
| Features & Benefits | • Determination of xanthine oxidase activity in serum, plasma, plant tissue/cell lysates and other biological fluids. |
| Usage notes | • Briefly centrifuge small vials at low speed prior to opening. • If not assayed immediately, samples can be stored at -80°C. |
| Storage instructions | Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Xanthine oxidase (EC 1.17.3.2), or xanthine oxidoreductase is recognized as the terminal enzyme of purine catabolism in humans, catalyzing the hydroxylation of hypoxanthine to xanthine and then to uric acid. When acting as an NADH oxidase, XO is a generator of superoxide (O2-), a powerful reactive oxygen species (ROS). XO has also been noted to produce hydrogen peroxide (H2O2) and superoxide (O2-) during ischemia-reperfusion injury. Due to their highly reactive nature, these ROS affect various molecular components of the cell, with excess amounts leading to cell degeneration and death. XO is present in appreciable amounts in the liver and jejunum in healthy individuals. However, in various liver disorders, levels of circulating XO have been seen to increase dramatically. Therefore, determination of serum XO level serves as a sensitive indicator of acute liver damage such as jaundice. |
活細胞和死細胞雙染色套組 | CCK-8細胞增殖和細胞毒性試劑盒 | LDH細胞毒性測定試劑盒 | 細胞衰老檢測試劑盒（β-半乳糖苷酶 | 細胞增殖EdU Image試劑盒
| 貨號Catalog no | 熱門商品推薦 |
| A21010 | HRP, Goat Anti-Mouse IgG |
| A21020 | HRP, Goat Anti-Rabbit IgG |
| A01050 | Anti-Plant Actin Mouse Monoclonal Antibody (3T3) |
| A23920 | Dylight 800, Goat Anti-Rabbit IgG |
| A25112 | IPKine™ HRP, Goat Anti-Mouse IgG HCS |
| A23220 | Dylight 488, Goat Anti-Rabbit IgG |
| A23420 | Dylight 594, Goat Anti-Rabbit IgG |
| A25022 | IPKine™ HRP, Mouse Anti-Rabbit IgG LCS |
| KET6017 | EliKine™ Human IL-6 ELISA Kit |
| A01090 | Anti-Lamin B1 Monoclonal Antibody (15T1) |
| A02010 | Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10) |
| A01030 | Anti-β-Tubulin Mouse Monoclonal Antibody (3G6) |
| A01060 | Anti-COX IV Mouse Monoclonal Antibody (14Y2) |
| A01070 | Anti-Histone H3 Mouse Monoclonal Antibody (2D10) |
| BMD00084 | AbFluor™ 594-Phalloidin |
| BMM3001 | Colorcode Prestained Protein Marker (10-180 kDa) |
| BMP1001 | Protease Inhibitor Cocktail (100X) |
| A23310 | Dylight 549, Goat Anti-Mouse IgG |
| BMR20100 | PurKine™ GST-Tag Glutathione Resin |
| BMR20206 | PurKine™ MBP-Tag Dextrin Resin 6FF |
| BMS0050 | Normal Goat Serum |
| A02020 | Anti-GFP Tag Mouse Monoclonal Antibody (3D3) |
| A02040 | Anti-HA Tag Mouse Monoclonal Antibody (4F6) |
| A02050MGB | Anti-His Tag Mouse Monoclonal Antibody (5C3), Magnetic Beads |
| A22110 | FITC, Goat Anti-Mouse IgG |
| A22120 | FITC, Goat Anti-Rabbit IgG |
| A23120 | DyLight 405, Goat Anti-Rabbit IgG |
| KTA4001 | Mitochondrial Membrane Potential Assay Kit (JC-1) |
| A23620 | Dylight 649, Goat Anti-Rabbit IgG |
| A01025 | Anti-GAPDH Mouse Monoclonal Antibody (2B5), HRP Conjugated |
| KET9007 | EliKine™ Rat TNF-α ELISA Kit |
| ABM0052 | α-SMA Monoclonal Antibody |
| ABM40111 | NFkB p65 Monoclonal Antibody |
| ABP0163 | PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody |
| ABP51894 | Na+/K+-ATPase α1 Polyclonal Antibody |
| A25012 | IPKine™ HRP, Goat Anti-Mouse IgG LCS |
| A25222 | IPKine™ HRP, Goat Anti-Rabbit IgG HCS |
| KTC4300 | TraKine™ Pro Live-cell Mitochondrion Staining kit (Deep Red Fluorescence with Super Resolution) |
| KTD3001 | Protein Quantification Kit (BCA Assay) |
| ABP57259 | GAPDH Polyclonal Antibody |
| BMD00007 | BCECF, AM ester |
| BMD00063 | DAPI |
| BMD00082 | AbFluor™ 488-Phalloidin |
| KET7005 | EliKine™ Mouse IL-1β ELISA Kit |
| KET6019 | EliKine™ Human IL-10 ELISA Kit |
| KTA3022 | Caspase-3 Assay Kit (Colorimetric) |
| KTB1050 | CheKine™ Lipid Peroxidation (MDA) Assay kit |
| BMS0140 | Normal Donkey Serum |
| K22020 | SuperLumia ECL HRP Substrate Kit |
| K22030 | SuperLumia ECL Plus HRP Substrate Kit |
| KET0001 | EliKine™ Testosterone ELISA Kit |
| KET6013 | EliKine™ Human IL-1β ELISA Kit |
| KTC4008 | TraKine™ F-actin Staining Kit (Green Fluorescence) |
| KTC4100 | TraKine™ Pro Live-cell Tubulin Staining kit (Green Fluorescence with Super Resolution) |
| KET7009 | EliKine™ Mouse IL-6 ELISA Kit |
| KTP4003 | ExKine™ Mitochondrion Extraction Kit (Cultured Cells) |
| KET0003 | EliKine™ Estradiol ELISA Kit |
| KTC011001 | Cell Counting Kit-8 (CCK-8) |
| KET9006 | EliKine™ Rat TGF-β1 ELISA Kit |
| KTA0002 | Annexin V-AbFluor™ 488 Apoptosis Detection Kit |
| KTA1001 | Live and Dead Cell Double Staining Kit |
| KTA3010 | Cell Senescence Kit (β-Galactosidase Assay) |
| K21010 | Protein Gel Flash Staining Kit |
| KTB1400 | CheKine™ Nitric Oxide (NO) Assay Kit |
| KTP3005 | ExKine™ Membrane and Cytoplasmic Protein Extraction Kit |
| KTB1100 | CheKine™ Lactate Assay Kit |
| KTB1310 | CheKine™ Glucose Oxidase Activity (GOD) Assay Kit |
| KTC4001 | TraKine™ Cell Plasma Membrane Staining Kit (Green Fluorescence) |
| KET6032 | EliKine™ Human TNF-α ELISA Kit |
| A24221 | IFKine™ Green Donkey Anti-Rabbit IgG |
| KTC4210 | TraKine™ Pro Live-cell Lysosome Staining kit (Deep Red Fluorescence with Super Resolution) |
| KTA1030 | LDH Cytotoxicity Assay Kit |
| KTD3002 | Protein Quantification Kit (Bradford Assay) |
| KTP21400 | PurKine™ Endotoxin Removal Kit (polymyxin B) |
| KET7015 | EliKine™ Mouse TNF-α ELISA Kit |
| ABP50010 | Cleaved-Caspase-9 p35 (D315) Polyclonal Antibody |
| KTL0520 | LinKine™ AbFluor™488 Labeling Kit |
| KTA3026 | Caspase-9 Assay Kit (Colorimetric) |
| A24421 | IFKine™ Red Donkey Anti-Rabbit IgG |
| K21010 | Protein Gel Flash Staining Kit |
| A02010AGB | Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10), Agarose |
| KTL0210 | LinKine™ FITC Labeling Kit |
| KTL0540 | LinKine™ AbFluor™ 594 Labeling Kit |
| BMR21504 | PurKine™ Anti-DDDDK Tag Resin 4FF |
| BMD00062 | Hoechst 33342 |
| BMS0070 | Normal Mouse Serum |
| KTA1030 | LDH Cytotoxicity Assay Kit |
| PRP100274 | Human IL-2 protein |
| PRP100400 | Mouse BDNF Protein |
| ABM0021 | GFAP Monoclonal Antibody |
| ABM0068 | PI3 Kinase P85α Monoclonal Antibody |
| KET6033 | EliKine™ Human VEGF ELISA Kit |
| KTE30164 | Chicken Marek’s disease virus IgG antibody (MDV-Ab-IgG) ELISA Kit |
| KTA2010 | TUNEL Apoptosis Detection Kit (Green Fluorescence) |
| PRP100159 | Human EGF protein |
| KET6011 | EliKine™ Human IFN-γ ELISA Kit |
| KET6027 | EliKine™ Human MMP-9 ELISA Kit |
| KTE62512 | Human Collagen Type I Alpha 1 (COL1A1) ELISA Kit |
| A01010 | Anti-β-Actin Mouse Monoclonal Antibody (1C7) |
| A01020 | Anti-GAPDH Mouse Monoclonal Antibody (2B5) |