CheKine™組織和血液鹼性磷酸酶（ Alkaline Phosphatase, AKP / ALP）比色測定試劑盒 | 貨號KTB1700

CheKine™ Tissue and Blood Alkaline Phosphatase (AKP/ALP) Colorimetric Assay Kit

產品描述： AKP / ALP（Alkaline Phosphatase）是一種含鋅的糖蛋白酶(zinc-containing glycoprotease)，可以在鹼性環境中水解各種天然和合成的磷脂單酯化合物。 AKP / ALP（Alkaline Phosphatase）廣泛分佈於人體的各個器官，主要是肝臟。在鹼性環境中，AKP / ALP（Alkaline Phosphatase）催化鄰苯二甲酸二鈉生成游離苯酚。苯酚與4-氨基安替比林和鐵氰化鉀反應生成紅色醌衍生物，其特徵是在510 nm處有光吸收。吸光度增加率反映了AKP活性。

Product name	CheKine™ Micro Tissue and Blood Alkaline Phosphatase (AKP/ALP) Activity Assay Kit
Applications notes	In an alkaline environment, AKP / ALP catalyzes phthalate disodium to generate free phenol; phenol reacts with 4-aminoantipyrine and potassium ferricyanide to produce a red quinone derivative, which has characteristic light absorption at 510 nm; the absorbance increase rate reflect AKP activity.
Kit components	• Extraction Buffer • Chromogen A • Chromogen B • Chromogen C • Standard
Features & Benefits	• Determination of alkaline phosphatase (AKP/ALP) activity in tissue lysates supernatants, plasma, serum, urine or purified enzyme samples. • Sample preparation, experimental steps and result calculation are more detailed and accurate. • Long shelf life.
Usage notes	• Assay kit is intended for research use only. Not for use in diagnostic procedures. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices. • Do not mix or substitute kit reagents or materials from other lots or vendors. Kits are QC tested as a set of components and performance cannot be guaranteed if utilized separately or substituted. • Avoid foaming or bubbles when mixing or reconstituting components. • Avoid cross-contamination, change pipette tips between additions of standards, samples and reagents. Also, use separate reservoirs for each reagent. • Ensure all reagents and equipment are at the appropriate temperature before assay. • Chromogen A, Chromogen B and Chromogen C should be kept away from light. Chromogen C cannot be used after it turns blue-green. • After adding Chromogen C, it must be mixed immediately, otherwise the color development will not be complete.
Storage instructions	Storage at 4°C and protected from light upon receipt. Kit has a storage time of 12 months form receipt.
Shipping	Gel pack with blue ice.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	AKP / ALP is a zinc-containing glycoprotease that can hydrolyze various natural and synthetic phospholipid monoester compounds in an alkaline environment. AKP / ALP is widely distributed in various organs of the human body, mainly the liver.

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貨號	產品名稱	胞器染色位置	螢光波段
貨號KTC4003	TraKine™粒線體染色	粒線體(mitochondria)	Ex / Em = 490/523 nm	增殖(proliferating)和非增殖細胞(non-proliferating cells)
貨號KTC4100	Pro活細胞微管蛋白染色	微管蛋白(Tubulin)	Ex/Em = 500/520 nm	活細胞或固定之細胞
貨號KTC4001	Abbkine 細胞膜染色	細胞膜 (plasma membrane)	Ex/Em = 484/501 nm	活細胞、固定懸浮、貼附細胞
貨號BMD00063	DAPI Staining Solution	細胞核 dsDNA	Ex/Em: 358/461 nm (with DNA)	染色活細胞和固定細胞
貨號KTC4210	活細胞Lysosome染色	Lysosome	deep red fluorescent dye , Ex/Em:650/665nm	染色活細胞和固定細胞