Catalase過氧化氫酶活性檢測 | CheKine™ Catalase Activity Assay Kit貨號KTB1040
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Catalase過氧化氫酶活性檢測試劑盒 | CheKine™ Catalase Activity Assay Kit貨號KTB1040
過氧化氫酶（Catalase , CAT）是幾乎在所有活生物體中常見的抗氧化酶(antioxidant enzyme)。它的主要功能是催化過氧化氫(hydrogen peroxide)分解為水(water)和氧氣(oxygen)，並去除體內的過氧化氫(hydrogen peroxide)，從而防止細胞被過氧化氫中毒。它是生物防禦系統(biological defense system)中的關鍵酶之一，為人體提供了抗氧化防禦機制(antioxidant defense mechanism)。
Fig. Formaldehyde standard curve. The y-axis is absorbance of standards and the x-axis is final formaldehyde concentration (uM).
產品描述：CheKine™過氧化氫酶活性檢測試劑盒(CheKine™ Catalase Activity Assay Kit, KTB1040) 可應用於各種生物樣品（例如Tissue Homogenate, Cell Lysate, serum, plasma, erythrocyte lysate）中的過氧化氫酶活性(catalase activity)檢測, 提供了一種簡便的比色測定方法。 該檢測試劑盒基於過氧化氫酶(catalase)與甲醇(methanol)的反應，在最佳濃度的H2O2存在下，利用過氧化氫酶(catalase)的過氧化功能來測量過氧化氫酶活性。 產生的甲醛(formaldehyde)可以在OD 540 nm處進行比色測定。 因此，樣品中存在的過氧化氫酶活性(catalase activity)與獲得的信號成正比。測量線性範圍為 2-75 µM, 最低測量活性為2 U/ml。
Abbkine對於CAT活性測定試劑盒(CAT Activity Assay Kit)，選擇了不同的品牌（Cayman Chemical，Abcam，Biovision），與Abbkine CheKine™過氧化氫酶（CAT）活性測定試劑盒（Cat＃：KTB1040）進行了比較。CAT活性測定試劑盒，Abbkine和Cayman Chemical品牌的測定原理相似。甲醇(Methanol)被用作反應底物。而Abcam和Biovision將H2O2作為反應底物。H2O2不僅是過氧化氫酶（catalase , CAT）的單一底物，樣品中的其他過氧化物酶可能會干擾實驗結果，並且標準曲線誤差可能是由過氧化氫的不穩定性引起的。儘管甲醇(Methanol)是過氧化氫酶的獨特底物，但其他過氧化物酶不能使用甲醇(Methanol)作為底物，因此可以避免訊號干擾。
| Brand | Assay Principle | Sample type |
| Abbkine | 過氧化氫酶活性(catalase activity)檢測, 提供了一種簡便的比色測定方法。 該檢測試劑盒基於過氧化氫酶(catalase)與甲醇(methanol)的反應，在最佳濃度的H2O2存在下，利用過氧化氫酶(catalase)的過氧化功能來測量過氧化氫酶活性。 產生的甲醛(formaldehyde)可以在OD 540 nm處進行比色測定。 因此，樣品中存在的過氧化氫酶活性(catalase activity)與獲得的信號成正比。測量線性範圍為 2-75 µM, 最低測量活性為2 U/ml。 | Tissue Homogenate, Cell Lysate, serum, plasma, erythrocyte lysate |
| Cayman Chemical | 該檢測試劑盒基於過氧化氫酶與甲醇的反應，在最佳濃度的H2O2存在下，利用過氧化氫酶的過氧化功能來測量過氧化氫酶活性。 產生的甲醛可以在OD 540 nm處進行比色測定。 因此，樣品中存在的過氧化氫酶活性與獲得的信號成正比。 | Tissue Homogenate, Cell Lysate, serum, plasma, erythrocyte lysate |
| Abcam | 樣品中存在的過氧化氫酶與過氧化氫（H2O2）反應生成水和氧氣。 未轉化的H2O2與探針反應生成可以在OD 570 nm處進行比色法測量的產物。 | Serum, Plasma, Cell Lysate, Tissue Lysate, Urine |
| Biovision | 樣品中存在的過氧化氫酶與過氧化氫（H2O2）反應生成水和氧氣。 未轉化的H2O2與探針反應生成可以在OD 570 nm處進行比色法測量的產物。 | Tissue Homogenate, Cell Lysate, erythrocyte lysate |
Kit components
• Assay Buffer
• Sample Diluent
• Formaldehyde standard (4.25 M)
• Catalase (control)
• Potassium Hydroxide
• Hydrogen Peroxide
• Chromogen
• Potassium Periodate
Features & Benefits
• Determination of catalase activity in serum, plasma, tissue/cell lysates and other biological fluids.
• Determining catalase activity directly by utilizing the peroxidatic function of catalase, which can not be interfered by other peroxidases.
• A broad range linearity: 2-75 µM.
• Measure catalase activity down to 2 U/ml
活細胞和死細胞雙染色套組 | CCK-8細胞增殖和細胞毒性試劑盒 | LDH細胞毒性測定試劑盒 | 細胞衰老檢測試劑盒（β-半乳糖苷酶 | 細胞增殖EdU Image試劑盒
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