超氧陰離子清除測定試劑盒| 貨號KTB1080

CheKine™ Superoxide anion Scavenging Capacity Assay Kit

超氧陰離子（superoxide anion , O2-）是通過將電子加到氧中而產生的一種短暫的自由基(short-lived radical production)。它是根據環境因素（例如紫外線，香煙煙霧，環境污染物和g射線輻射）形成的，或衍生自黃嘌呤氧化酶或NADPH氧化酶等氧化酶。 O2－一旦形成，就會攻擊細胞成分並破壞脂質，蛋白質和DNA。這會引發多種疾病，包括癌症(diseases)，動脈粥樣硬化(atherosclerosis)，類風濕性關節炎(rheumatoid arthritis)，糖尿病(diabetes)，肝損害(liver damage)和中樞神經系統疾病(central nervous system disorders)。CheKine™超氧陰離子清除測定試劑盒(CheKine™ Superoxide anion Scavenging Capacity Assay Kit)為研究組織/細胞裂解液和其他生物液中的超氧陰離子清除能力，提供了一種簡便的比色測定法。在該測定中，黃嘌呤氧化酶（xanthine oxidase , XO）催化反應提供了超氧陰離子（superoxide anion , O2-）。 O2-與四唑鎓鹽WST-8染料反應形成水溶性的有色formazan產物，可以很容易地進行比色定量。樣品清除了O2－，因此用於顯色反應的O2－較少。通過比色法在OD 450nm下測量樣品的這種清除能力。

Product name	CheKine™ Micro Superoxide anion Scavenging Capacity Assay Kit
Applications notes	CheKine™ Micro Superoxide anion Scavenging Assay Kit provides a simple and easy colorimetric assay for the study of Superoxide anion Scavenging ability in tissue/cell lysates and other biological fluids. In this assay, superoxide anion (O2－) is provided by xanthine oxidase (XO) catalyzed reaction. O2－ reacts with a tetrazolium salt WST-8 dye to form a water-soluble colored formazan product, which can be easily quantified colorimetrically. Sample scavenges the O2－ thus less O2－ is available for the chromogenic reaction. This scavenging ability of sample is measured by colorimetric method at OD 450 nm.
Kit components	• Assay Buffer • Sample Diluent • WST-8 • Enhancer • Xanthine Oxidase • Xanthine
Features & Benefits	• Determination the Superoxide anion Scavenging ability in various samples, including serum, plasma, plant tissue/cell lysates and other biological fluids.
Usage notes	• Briefly centrifuge small vials at low speed prior to opening. • If not assayed immediately, samples can be stored at -80°C.
Background	The superoxide anion (O2－) is a short-lived radical production by the addition of an electron to oxygen. It is formed in response to environmental factors such as UV light, cigarette smoke, environmental pollutants, and g-radiation, or derived from oxidases like xanthine oxidase or NADPH oxidase. Once formed, O2－ attacks cellular components and causes damage to lipids, proteins and DNA. This can initiate numerous diseases, including cancer, atherosclerosis, rheumatoid arthritis, diabetes, liver damage, and central nervous system disorders. The study of scavenging superoxide anion radicals has been gained more and more attention.

引用文獻  Figure 3. After LPS treatment, peroxisome level decreases more significantly in ISOC1-deficient cells than in wild-type cells. Wild-type or ISOC1-deficient RAW264.7 cells were treated with LPS (300 ng/mL) for 24 h. (A) Peroxisomes were labeled with ABCD3 and were observed with fluorescence microscopy. Scale bar = 10 μm. Mean gray values were analyzed by Image J v1.51 (Wayne Rasband, Bethesda, MD, USA). (B) Cell lysates were subjected to WB with antibodies to ABCD3, PEX11B, and PEX1. (C) O2—scavenging capacity was measured using a superoxide anion scavenging capacity assay Kit. (D) The content of 8-OHDG was analyzed by an oxidative RNA Damage ELISA Kit. All data are shown as mean ± s.e.m (n = 3). * p < 0.05, ** p < 0.01; n.s. not significant. Two-way ANOVA followed by Bonferroni post hoc test was used for data analysis.

引用文獻  Figure 4. ISOC1 affects peroxisomes through the AKT1 pathway but not the NF-κB pathway. Wild-type or ISOC1-deficient RAW264.7 were pretreated with 10 μM Perifosine for 12 h or 10 μM BAY 11-7082 for 1.5 h, stimulated with 300 ng/mL LPS for 24 h. (A,E) Peroxisomes were labeled with ABCD3 and were observed with fluorescence microscopy. Scale bar = 10 μm. Mean gray value were analyzed by Image J. (B,F) WB was used to detect the expression levels of ABCD3, PEX11B, and PEX1. (C,G) O2− scavenging capacity was measured using a superoxide anion scavenging capacity assay Kit. (D,H) The content of 8-OHDG was analyzed by an oxidative RNA Damage ELISA Kit. All data are shown as mean ± s.e.m (n = 3). * p < 0.05, ** p < 0.01, n.s. not significant. Two-way ANOVA followed by Bonferroni post hoc test was used for data analysis.

活細胞和死細胞雙染色套組  |  CCK-8細胞增殖和細胞毒性試劑盒  | LDH細胞毒性測定試劑盒 | 細胞衰老檢測試劑盒（β-半乳糖苷酶 | 細胞增殖EdU Image試劑盒 

貨號	產品名稱	胞器染色位置	螢光波段
貨號KTC4003	TraKine™粒線體染色	粒線體(mitochondria)	Ex / Em = 490/523 nm	增殖(proliferating)和非增殖細胞(non-proliferating cells)
貨號KTC4100	Pro活細胞微管蛋白染色	微管蛋白(Tubulin)	Ex/Em = 500/520 nm	活細胞或固定之細胞
貨號KTC4001	Abbkine 細胞膜染色	細胞膜 (plasma membrane)	Ex/Em = 484/501 nm	活細胞、固定懸浮、貼附細胞
貨號BMD00063	DAPI Staining Solution	細胞核 dsDNA	Ex/Em: 358/461 nm (with DNA)	染色活細胞和固定細胞
貨號KTC4210	活細胞Lysosome染色	Lysosome	deep red fluorescent dye , Ex/Em:650/665nm	染色活細胞和固定細胞

 

產品貨號	品項	反應	測量儀器
貨號KTA1001	活細胞和死細胞雙染色試劑盒	100個反應	活細胞綠色螢光染劑Ex/Em = 488/530 nm; 死細胞紅色螢光染料(Ex/Em = 535/617)
貨號 KTA2010	TUNEL細胞凋亡檢測試劑盒	50個反應	螢光酶標儀、螢光顯微鏡或流式細胞儀。FITC通道（Ex / Em = 490nm / 520 nm）
貨號 KTC011001	CCK-8細胞增殖和細胞毒性試劑盒	500個反應	吸收光450nm
貨號KTC4003	TraKine™粒線體染色	50個反應/250個反應	Ex / Em = 490/523 nm增殖(proliferating)和非增殖細胞(non-proliferating cells)
貨號KTC4100	Pro活細胞微管蛋白染色	50個反應/250個反應	Ex/Em = 500/520 nm
貨號KTC4001	Abbkine 細胞膜染色	100個反應/500個反應	Ex/Em = 484/501 nm
貨號BMD00063	DAPI Staining Solution	10MG/100MG	Ex/Em: 358/461 nm (with DNA)
貨號KTC4210	活細胞Lysosome染色	50個反應/250個反應	deep red fluorescent dye , Ex/Em:650/665nm
貨號KTA3030	細胞衰老檢測試劑盒（β-半乳糖苷酶）	100個反應	光學顯微鏡
貨號KTA1020	CCK-8細胞增殖和細胞毒性試劑盒	1000個反應/10,000個反應	吸收光450nm
貨號KTA2030	細胞增殖EdU Image試劑盒 (BrdU替代法)	100個反應/500個反應	AbFluor 488 azide (Ex/Em = 501/525 nm)
貨號KTB1400	一氧化Dan（NO）檢測試劑盒	96個反應/ 192個反應	colorimetric (540 nm) 檢測範圍：1-100 µM
貨號KTB1070	CheKine™黃嘌呤氧化酶檢測	96個反應/ 480個反應	OD 450 nm 線性範圍15.6 -500 mU/
貨號KTD3001	BCA蛋白定量試劑盒	2000個反應/ 5000個反應	OD 562 nm 相容性 5 % SDS, 5 % Triton X-100, 5 % Tween-20, 60, 80 50 to 1000 µg/ml.
貨號KTB1110	乳酸脫氫酶（LDH）測定試劑盒	96個反應/ 480個反應	λmax= 450 nm 1- 20 U/mL of LDH
貨號PRP100153	IGF-I重組蛋白	200UG/1MG	human IGF1 isoform 1 (P05019-1) (Gly 49-Ala 118) > 95 % as determined by SDS-PAGE
貨號 KTA2020	細胞週期染色檢測	100個反應/500個反應	Ex/Em=535/615 nm.
貨號BMC21400	PurKine™內毒素去除預裝離心柱	1ml×5 3mlx5	More than 2,000,000 EU/mL to eliminate >99% of endotoxins.
貨號KTA1200	CheKine™蛋白羰基比色測定試劑盒	48個反應/ 96個反應	吸收光370 nm
貨號KTB1080	超氧陰離子清除測定試劑盒	48個反應/ 96個反應	Superoxide anion scavenging rate D% =（∆∆ODControl – ∆∆ODSample）÷ ∆∆ODControl ×100%
貨號KTB1090	清除氫氧自由基能力測定試劑	48個反應/ 96個反應	吸收光 536nm Hydroxyl radical scavenging rate D% =（ASample – AControl）÷（ABlank – AControl）×100%
貨號KTB2220	TC總膽固醇檢測試劑盒	48個反應/ 96個反應	500nm Standard: 5 μmol/mL
貨號KTB1510	CheKine™尿酸（UA）比色分析試劑盒	48個反應/ 96個反應	吸收光505 nm Standard: 5 μmol/mL.
貨號KTB1300	Glucose葡萄糖測定試劑盒	96個反應/ 192個反應	吸光值630nm 4- 300 mg/dL)
貨號KTB1100	Lactate  乳酸檢測試劑盒	96個反應/ 480個反應	colorimetric (450 nm) 0.03 mM-2 mM
貨號KTB1700	組織和血液鹼性磷酸酶（ Alkaline Phosphatase, AKP / ALP）比色測定	96個反應/ 96 X5盤	吸收光510 nm
貨號KTB1500	總抗氧化劑能力TAC分析	96個反應/ 480個反應	593 nm 偵測範圍: 0.15-3 mM
貨號KTA0002	Annexin V-AbFluor™488細胞凋亡檢測試劑盒	20個反應/50個反應/100個反應	Annexin V- AbFluor™ 488: Abs/Em = 491/517 nm; PI: Abs/Em = 535/617 nm (with DNA)
貨號KET7013	EliKine™ Rat IL-6 ELISA Kit試劑盒	96個反應/ 96 X5盤	450 nm 偵測範圍: 250pg/mL-16000 pg/mL, 最低靈敏度100 pg/mL
貨號KET0003	雌二醇（Estradiol , E2）試劑盒	96個反應/ 96 X5盤	450 nm 偵測範圍40 pg/ml-1500 pg/ml
貨號KET6032	TNF–α Alpha型腫瘤壞死因子 ELISA試劑盒	96個反應/ 96 X5盤	450 nm 7.8 pg/ml-500 pg/ml
貨號KET6033	VEGF血管內皮生長因子ELISA試劑盒	96個反應/ 96 X5盤	450 nm 偵測範圍: 31.25 pg/mL-2000 pg/mL
貨號KET7005	白介素-1β（Interleukin-1β, IL-1β）ELISA試劑盒	96個反應/ 96 X5盤	Microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm. 偵測範圍: 15.6 pg/ml-1000 pg/ml
貨號KTE70003	小鼠黃嘌呤脫氫酶/氧化酶（XDH）ELISA試劑盒	96個反應/ 96 X5盤	450 nm 偵測範圍: 22.5 ng/L-360 ng/L, 最低靈敏度1.0 ng/L。
貨號 A02051HRP	His-tag標籤多株抗體-HRP	50ul/200ul	WB (1:5000).
貨號A02170MGB	Anti-V5 Tag Mouse Monoclonal Antibody (11D5), 磁珠	100ul/400ul/2ml	GKPIPNPLLGLDST epitope
貨號KTA4001	JC-1線粒體膜電位測定試劑盒	100個反應/ 500個反應	（聚結的JC-1，Ex / Em = 585/590 nm）發出綠色螢光（JC-1單體，Ex / Em = 510/527 nm） JC-1 Stain CCCP (10 mM)  Assay Buffer (5×)
貨號KTB1600	穀胱甘肽(Glutathione)檢測	96個反應	412 nm 2-200 µg/mL
貨號BMD00063	DAPI Staining Solution	1MG/10MG/100MG	λEx/λEm: 358/461 nm (with DNA)
貨號KTP3001	細胞核/細胞質分離試劑盒	50個反應/ 200個反應	Cytoplasmic Solution A (CES A) Cytoplasmic Solution B (CES B) • Nuclear Extraction Solution (NES) DTT (500X) Protease Inhibitor (100X)
貨號BMD00064-50UG	Calcein AM鈣黃綠素活細胞螢光染劑	50ug/1mg/10mg	λEX/λEm: 494/517 nm (pH 8) (calcein)
貨號 KTB1660	硫氧還蛋白過氧化物酶（TPX）檢測	96個反應	240nm
貨號KTB1020	NAD/NADH 分析試劑盒	96個反應/ 480個反應	OD565 nm 96 孔板測定中的檢測限值為 0.78 μM 線性最高 50 μM NAD+/NADH。
貨號KTB1070	CheKine™黃嘌呤氧化酶檢測	96個反應/ 480個反應	OD 450 nm 線性範圍15.6 -500 mU/mL
貨號KTB1040	Catalase過氧化氫酶活性檢測試劑盒	96個反應/ 480個反應	OD 540 nm 2-75 µM
貨號KTB1060	過氧化物酶（Peroxidases）分析試劑盒	96個反應	OD 664 nm 線性範圍為15.6-1000 µU/mL
貨號KTB1050	脂質過氧化檢測試劑盒(MDA)	96個反應	OD 532 nm 1-100 µM/L
貨號KTB1030	超氧化物歧化酶（SOD）活性測定試劑盒	96個反應	200-3.13 U/ml
貨號KTA1030	LDH細胞毒性測定試劑盒	96個反應/ 480個反應	OD 565 nm 10,00-100,0000 cells per well