CheKine™蛋白羰基比色測定試劑盒 | Protein Carbonyl Colorimetric Assay Kit貨號KTB1200
| 代理廠牌： |  |
| 原廠連結： | |
| 相關下載： |
CheKine™蛋白羰基比色測定試劑盒 | 貨號KTB1200
CheKine™ Protein Carbonyl Colorimetric Assay Kit
羰基蛋白(Protein carbonyl)是蛋白質氧化修飾(protein oxidative modification)過程中多種氨基酸(amino acids)的早期信號，其含量表明了蛋白質的氧化損傷程度(protein oxidative damage degree)，是衡量蛋白質氧化損傷(protein oxidative damage)的主要指標(indicator)。羰基(carbonyl group)與2,4-二硝基苯(2,4-dinitrophenylhydrazine, DNPH )反應生成紅色的2,4-二硝基苯(2,4-dinitrophenylhydrazone)，在370 nm處具有特徵吸收峰。操作手冊 CheKine™ Protein Carbonyl Colorimetric Assay Kit
商品需用儀器測量 酵素免疫分析儀請按此 (標配405、450、492、630 nm, 可擴增至7個濾片)
- Kit components
- Extraction Buffer
• Antioxidant
• Chromogen
• HCL
• TCA
• Guanidine Hydrochloride - Features & Benefits
- Determination of Protein carbonyl content in cells, bacteria, serum (plasma), tissue/cell lysates and other biological fluids.
• Sample preparation, experimental steps and result calculation are more detailed and accurate.
• Long shelf life.
活細胞和死細胞雙染色套組 | CCK-8細胞增殖和細胞毒性試劑盒 | LDH細胞毒性測定試劑盒 | 細胞衰老檢測試劑盒（β-半乳糖苷酶 | 細胞增殖EdU Image試劑盒
Sample Preparation
1. Animal and Plant Tissue samples: Weigh 0.1 g tissue, add 1 mL Extraction Buffer and homogenize on ice. Centrifugeat 4,000g for 10 min at 4°C. Take the supernatant, add 0.1 mL Working Antioxidant, keep at room temperature for 10 min, centrifugeat10,000 g for 10 min at 4°C. Use supernatant for assay, and place it on ice to be tested.
2. Cells or Bacteria: Collect 5×106 cells or bacteria into the centrifuge tube, wash cells or bacteria with cold PBS, discardthesupernatant after centrifugation; add 1 mL Extraction Buffer to ultrasonically disrupt the cells or bacteria 5 min (power 20%or 200W, ultrasonic 3 s, interval 7 s, repeat 30 times). Centrifuge at 10,000 g for 10 min at 4°C. Use supernatant for assay, andplaceiton ice to be tested.
3. Serum (Plasma) sample: Tested directly
| 貨號 | 產品名稱 | 胞器染色位置 | 螢光波段 | |
| 貨號KTC4003 | TraKine™粒線體染色 | 粒線體(mitochondria) | Ex / Em = 490/523 nm | 增殖(proliferating)和非增殖細胞(non-proliferating cells) |
| 貨號KTC4100 | Pro活細胞微管蛋白染色 | 微管蛋白(Tubulin) | Ex/Em = 500/520 nm | 活細胞或固定之細胞 |
| 貨號KTC4001 | Abbkine 細胞膜染色 | 細胞膜 (plasma membrane) | Ex/Em = 484/501 nm | 活細胞、固定懸浮、貼附細胞 |
| 貨號BMD00063 | DAPI Staining Solution | 細胞核 dsDNA | Ex/Em: 358/461 nm (with DNA) | 染色活細胞和固定細胞 |
| 貨號KTC4210 | 活細胞Lysosome染色 | Lysosome | deep red fluorescent dye , Ex/Em:650/665nm | 染色活細胞和固定細胞 |