BCA總蛋白定量20-2000μg/ml | Protein Quantification Kit Abbkine 貨號KTD3001
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特點
Abbkine BCA蛋白定量試劑盒 | 貨號KTD3001 20-2000 µg/ml
The BCA method is suitable for the detection of protein concentrations in the range of 20-2,000 µg/mL, the minimum detected protein amount is 0.4 µg, and the sample volume to be tested is 1-20 µL.
Abbkine 蛋白定量試劑盒 (BCA 分析) 為總蛋白質的色度檢測和定量提供了一個簡單、快速、與洗滌劑相容的程式。該方法利用鹼性介質中蛋白質減少銅陽離子(Cu2+)到銅陽離子(Cu+)。生成的 Cu+ 與二面體酸 (BSA) 形成強烈的紫色複合物,在 562nm 時具有極強的吸收力。水溶性複合物的強度與樣品中的蛋白質量成正比。BCA蛋白測定適用於20-1000μg/ml範圍內的蛋白質濃度測量。BCA (Bicinchoninic Acid , BCA) 在鹼性環境Cu+ 由鹼性Cu2+蛋白質的反應產生的。由此產生的反應和顏色是一種普通蛋白質定量方法的基礎,該方法能夠測量各種蛋白質濃度。增加蛋白質濃度會產生比例更深的顏色。BCA蛋白測定與勞里技術相比,BCA蛋白測定對普通干擾物質(如非離子洗滌劑和緩衝鹽)的耐受性更高。
This product has been cited in 44 publications
Abbkine 蛋白定量試劑盒產品特點:
- 節省時間: 比傳統的Lowry method方法更容易,快四倍。
- 靈敏度: 檢測低至20 µg/mL，最低檢測蛋白量達到0.4 µg。
- 相容性: 不受大多數離子和非離子洗滌劑的影響。可與高達 5% SDS、5% Triton X-100、5% Tween-20、60、80 兼容。
- 良好的線性度: 線性工作範圍從 20 到2000 μg/ml.
- 與染料結合方法相比,蛋白質到蛋白質的變異性較少。
Fig. Abbkine Protein Quantification Kit (BCA Assay) standard curve
| Product name | Protein Quantification Kit (BCA Assay) |
| Applications notes | The BCA method is suitable for the detection of protein concentrations in the range of 20-2,000 µg/mL, the minimum detected protein amount is 0.4 µg, and the sample volume to be tested is 1-20 µL. |
| Detection method | Colorimetric |
| Alternative | Bicinchoninic acid protein |
| Kit components | • BCA Reagent A • BCA Reagent B • BSA Standard • BSA Standard Buffer |
| Features & Benefits | • Time saving—much easier and four times faster than the classical Lowry method. • Sensitive—detect down to 20 µg/mL and the minimum amount of detection protein reaches 0.4 μg. • Compatible—unaffected by most ionic and nonionic detergents. Can be compatible with up to 5% SDS, 5% Triton X-100, 5% Tween-20, 60, 80 in the sample. • Good linearity—linear working range from 20 to 2000 µg/mL. • Excellent uniformity— exhibits less protein-to-protein variation than dye-binding methods. |
| Calibration range | 20-2,000 µg/mL |
| Limit of detection | 20 µg/mL |
| Usage notes | • 1. Use a microplate reader to measure the absorbance at 562 nm, or the absorbance at other wavelengths between 540-595 nm. • 2. The protein concentration determined by BCA method is not affected by chemical substances in most samples, and can be compatible with up to 5% SDS, 5% Triton X-100, 5% Tween20, 60, 80 in the sample. However, the BCA method is affected by chelating agents and slightly higher concentrations of reducing agents. It is necessary to ensure that EDTA is less than 10 mM, no EGTA, dithiothreitol (DTT) is less than 1 mM, and β-mercaptoethanol (β-Mercaptoethanol) is less than 0.01%. • 3. It is recommended to make a standard curve for each measurement. Because the color of the BCA method will continue to deepen with the extension of time, and the color reaction will be accelerated due to the increase of temperature. • 4. If the sample diluent or lysate itself has a high background, please try the Protein Quantification Kit (Bradford Assay) (Cat #:KTD3002). |
| Storage instructions | Store at room temperature, stable for 12 months |
| Shipping | Room temperature transport |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. |
引用文獻
YL Zhang, C Geng, J Yang, J Fang, X Yan, PB Li. Biochimica et Biophysica Acta (BBA) – Molecular Basis of Disease, 2019.
技術提示
為避免交叉污染，添加試劑之間，添加樣品之間都需更換Tip。另外，每種試劑都應使用單獨的容器。BCA試劑B與BCA試劑A稀釋液混合後，觀察到渾濁混合後迅速消失，得到透明的綠色BCA工作溶液。BCA孵育時間和溫度都可以在相當廣泛的範圍內變化。使用更高的溫度和更長的孵育時間，蛋白質含量較低的樣品更容易定量。已知某些物質會干擾BCA分析，包括那些降低電位，螯合劑和強酸或強鹼。因為他們是已知即使在極低的濃度下也會干擾蛋白質估計，避免這些物質作為樣品緩衝液的成分。某些物質在較小程度上干擾了使用BCA進行蛋白質估算分析，並且在低於特定濃度的情況下，這些僅具有較小的（可忍受的）影響，如NP-40，SDS，Triton X-100，Tween-20等。在含有去污劑的溶液中測定蛋白質時，可通過以下方法獲得最佳結果向含有蛋白質標準品的孔中添加相同量的去污劑。
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