CheKine™總抗氧化劑能力分析 | CheKine™ Total Antioxidant Capacity (TAC) Assay Kit貨號KTB1500  偵測範圍: 0.15-3 mM

偵測範圍: 0.15-3 mM

Fig. Standard Curve of Total Antioxidant Capacity (TAC) Assay. The y-axis is OD593 and the x-axis is Fe2+ concentration (mM).

抗氧化劑(Antioxidants)在防止自由基和其他潛在有毒氧化物質的形成和清除方面(scavenging of free radicals )發揮著重要作用。抗氧化劑(antioxidant species)種類有三類：酵素系統穀胱甘肽還原酶(GSH reductase)、過氧化氫酶(catalase)、過氧化物酶(peroxidase)等、小分子抗壞血酸(ascorbate, uric acid)、尿酸(uric acid)、穀胱甘肽(GSH)、維生素E等及蛋白質白蛋白(albumin)、轉鐵蛋白( transferrin)等。CheKine™總抗氧化劑能力（Total Antioxidant Capacity , TAC）測定試劑盒提供了一種簡單易用的比色測定法，用於測量血清，血漿，細胞培養上清液，尿液，組織/細胞裂解液和其他生物流體中的總抗氧化劑能力。 在該測定中，Fe3 + -TPTZ被抗氧化劑還原為Fe2 + -TPTZ。 酶催化的反應產物Fe2 + -TPTZ可以在比色讀數為593 nm的條件下進行測量。偵測範圍: 0.15-3 mM。可應用於以下樣品來源: serum, plasma, cell culture supernatants, urine, tissue/ cell lysates and other biological fluids.

 

(文獻引用出自) The total antioxidant capacity (TAC) and hydroxyl free radical scavenging capacity assays revealed the ability to remove H₂O₂ and –OH, reflecting the antioxidant ability of the targeted reagent. The results in Figure 4A indicated that the moderate combination with peptide at 600:1 did not weaken the total antioxidant capacity of tFNA.

(改良版) KTB1500試劑盒升級過程中，考慮到這個標曲在很多種情況下，是一個比較恆定的的方程；為了方便操作，省略客戶自己製作標曲的步驟簡潔 (本套組沒有標準品, 只能提供Positive control) ，提供了一個方程，讓客戶直接帶入方程計算即可；在數值計算上和原來沒有任何區別，只是省去了自己製作標曲的步驟；客戶使用起來更方便、省時。
Standard curve Calculation formula of TAC: y=4.4664x+0.0685, R2=0.9986, y is Fe
2+ concentration (μmol/mL), x is theabsorbance change value (ΔA), Substitute the ΔATest into the equation to obtain the y value (µmoL/mL).

Ktb1500 Positive Control溶解後的保存時間(4度C), Ascorbic Acid (Positive Control)  建議儘量1周內用完; 如不立即使用, 4℃可以保存1個月，分裝保存在-20℃, 可儲存2-3個月; 若溶液變成棕色，說明組分變質.

Kit components	• Assay Buffer (10X) • Substrate Diluent • Substrate • Reaction Buffer • ascorbic acid (positive control)
Features & Benefits	• Determination of Total Antioxidant Capacity in serum, plasma, cell/tissue samples, urine and bacteria samples. • Using a stronger antioxidant, sascorbic acid as positive control, instead of the frequently-used Trolox. • The reaction is carried out under acidic conditions, which can inhibit some endogenous interference factors.
Usage notes	• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range. • Fresh samples are necessary for good results. If not assayed immediately, samples can be stored at -80°C for one month. • Can not use EDTA as the anticoagulant for serum and plasma samples. The samples also can not contain DTT, Mercaptoethanol, Tween, Triton, NP-40.
Storage instructions	Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Shipping	Gel pack with blue ice.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.