穀胱甘肽(Glutathione)檢測 | CheKine™ Reduced Glutathione (GSH) Colorimetric Assay Kit貨號KTB1600

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相關下載：	穀胱甘肽(Glutathione)檢測操作手冊

特點

穀胱甘肽(Glutathione)檢測 | CheKine™ Reduced Glutathione (GSH) Colorimetric Assay Kit貨號KTB1600 偵測靈敏度2-200 µg/mL.

穀胱甘肽 (Glutathione) 研究的意義

穀胱甘肽是含有谷氨酸 (glutamate)，半胱氨酸(cysteine)和甘氨酸(glycine)的三肽，其中含有巰基(sulfhydryl groups)。它廣泛存在於動植物中。它具有抗氧化劑(antioxidant)和綜合解毒(detoxification)作用。其相關的輔酶(coenzyme)是NADPH。人體新陳代謝產生的自由基(free radicals)過多會破壞生物膜(biofilm)，侵入生命大分子，加速人體衰老(aging)，並誘發腫瘤(tumors)或動脈粥樣硬化(atherosclerosis)的產生。穀胱甘肽具有增強生物體的抗氧化功能，促進生長以及增強生物體對人體不利環境的影響的功能。它的主要生理作用是清除體內的自由基。它也是一種很好的解毒劑，可以減弱有毒物質的毒性並從體內清除有毒物質。穀胱甘肽還促進生長並提高人體免疫力。穀胱甘肽(Glutathione)不僅可以用作藥物，還可以用作功能性食品的基礎材料，並且廣泛用於功能性食品中，例如抗衰老(anti-aging) ，增強免疫力(enhancing immunity)和抗腫瘤作用(anti-tumor)。

穀胱甘肽有兩種形式：還原型（GSH）和氧化型（GSSG）。氧化穀胱甘肽(Glutathione oxidized)，也稱為二硫代穀胱甘肽(Dithioglutathione)，其是通過兩個還原的穀胱甘肽分子的氧化形成的。穀胱甘肽還原酶將GSSG還原為GSH。還原型穀胱甘肽存在於大量生物中，並起主要作用。在紅血球細胞中，還原型穀胱甘肽（GSH）是保持血紅蛋白處於還原狀態並保護細胞免受氧化損傷的關鍵。 GSH是細胞中最重要的抗氧化劑巰基化合物，在抗氧化性，蛋白質巰基保護和氨基酸跨膜運輸中起重要作用。還原型和氧化型穀胱甘肽之間的比例（GSH / GSSG）是細胞氧化還原狀態的主要指標。因此，測量細胞中GSH和GSSG的含量以及GSH / GSSG的比值可以反映細胞的氧化還原狀態，但是其他具有過氧化活性的酶卻沒有利用這些底物。穀胱甘肽(Glutathione)檢測 (貨號KTB1600) 套組: 原理DTNB與還原型穀胱甘肽反應形成黃色產物。在412 nm處測得的光密度可以直接反映樣品中的穀胱甘肽濃度。

(文獻引用) Mep-S inhibits H₂O₂-induced ROS generation and decreases GSH content and SOD activity in SH-SY5Y cells. (a) Mep-S inhibited H₂O₂-induced intracellular ROS accumulation, as demonstrated by decreased DCFH-DA fluorescence intensity. Scale μm. Mep-S inhibited the H₂O₂-induced decrease in GSH content (b) and SOD activity (c). The cells were pretreated with Mep-S (0.5-2 μM) or NAC (1 mM) for 1 h prior to stimulation with H₂O₂ (400 μM) for 2 h (a) or 24 h (b, c). Data are presented as the of 2-4 independent experiments.

(文獻引用) Figure 7. (a–d) Role of C. papaya on enzymatic antioxidants levels. p < 0.05 was considered statistically significant among the groups: *-control; #-diabetes; $-C. papaya-treated T2DM.

Kit components
Extraction Buffer
• Assay Buffer
• Chromogen
• Standard
Features & Benefits
Determination of Reduced Glutathione concentration in serum, plasma, tissue/cell lysates and other biological fluids.
• A broad range linearity: 2-200 µg/mL.
• Measure concentration down to 2 µg/mL.
Usage notes
The sample can be kept at -80°C for 10 days. If not do experiments immediately.
• Always prepare fresh standards per use.
• Diluted standard solution is unstable and must be used within 4 hours.
Storage instructions

Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components.

Shipping

Gel pack with blue ice.

CAT#	Product name	classification
KTB1600	CheKine™ Reduced Glutathione (GSH) Colorimetric Assay Kit	Cell metabolism
KTB1610	CheKine™ Glutathione Oxidized (GSSG) Colorimetric Assay Kit	Cell metabolism
KTB1620	CheKine™ Glutathione Reductases (GR) Colorimetric Assay Kit	Cell metabolism
KTB1630	CheKine™ Glutathione S-Transferase (GST) Colorimetric Assay Kit	Cell metabolism
KTB1640	CheKine™ Glutathione Peroxidase (GSH-Px) Colorimetric Assay Kit	Cell metabolism
KTB1650	CheKine™ Thioredoxin Reductase (TrxR) Colorimetric Assay Kit	Cell metabolism
KTB1660	CheKine™ Thioredoxin Peroxidase (TPX) Colorimetric Assay Kit	Cell metabolism

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