DAPI Staining Solution (粉狀)| 細胞核染劑CAS#28718-90-3貨號BMD00063
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特點
DAPI Staining Solution 粉狀 (DNA staining) | 貨號BMD00063 (10mg/100mg)
產品描述： DAPI是一種流行的藍色螢光DNA染料(blue fluorescent DNA dye)。該染料與dsDNA的結合，螢光增強約20倍。由於DAPI穿過完整的細胞膜，因此可用於染色活細胞和固定細胞。DAPI通常用於細胞凋亡檢測，染色後將使用螢光顯微鏡(fluorescence microscope)或流式細胞儀(flow cytometer)進行檢測。DAPI的藍色發射便於進行多重分析，因為DAPI與綠色螢光分子（如FITC和綠色螢光蛋白（GFP）或紅色螢光染料（如Texas Red）之間幾乎沒有螢光重疊。
Fig. DAPI structure formula
文獻引用 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8434706/
文獻引用https://labanimres.biomedcentral.com/articles/10.1186/s42826-023-00157-4#citeas
文獻引用 https://www.nature.com/articles/s41467-023-40081-8#citeas
| Product name | DAPI |
| CAS Number | 28718-90-3 |
| Molecular Formula | C16H17Cl2N5 |
| Alternative | DAPI,BMD00063 |
| Formulation | Yellow powder |
| Features & Benefits | • λEx/λEm: 360/460 nm (with DNA) |
| Molecular weight | 350.25 |
| Usage notes | • Soluble in H2O |
| Storage instructions | Store at -20°C and protect from light. Product is stable for at least 12 months from date of receipt if stored as recommended. |
| Shipping | Gel pack with blue ice. |
| Background | DAPI is a popular blue fluorescent DNA dye. The dye binds to the minor groove of dsDNA with approximately 20-fold fluorescence enhancement. DAPI is often applied to apoptosis detection, which will use fluorescence microscope or flow cytometer after staining. |
| Alternative | DAPI,BMD00063 |
Publications(12)
Ruilian Li, Xianghua Yuan, Jinhua Wei, et al. Mar Drugs. 2019 Jan 10;17(1). pii: E43.
| 貨號 | 產品名稱 | 胞器染色位置 | 螢光波段 | |
| 貨號KTC4003 | TraKine™粒線體染色 | 粒線體(mitochondria) | Ex / Em = 490/523 nm | 增殖(proliferating)和非增殖細胞(non-proliferating cells) |
| 貨號KTC4100 | Pro活細胞微管蛋白染色 | 微管蛋白(Tubulin) | Ex/Em = 500/520 nm | 活細胞或固定之細胞 |
| 貨號KTC4001 | Abbkine 細胞膜染色 | 細胞膜 (plasma membrane) | Ex/Em = 484/501 nm | 活細胞、固定懸浮、貼附細胞 |
| 貨號KTC4210 | 活細胞Lysosome染色 | Lysosome | deep red fluorescent dye , Ex/Em:650/665nm | 染色活細胞和固定細胞 |
| 貨號BMD00063 | DAPI Staining Solution | 細胞核 dsDNA | Ex/Em: 358/461 nm (with DNA) | 染色活細胞和固定細胞 |
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