CCK-8細胞增殖和細胞毒性試劑盒 | Cell Counting Kit-8 (CCK-8) ABBKINE貨號KTA1020
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CCK-8細胞增殖和細胞毒性試劑盒 | 貨號 KTA1020 吸收光450 nm CCK8操作手冊
CCK-8; WST-8; CCK8; WST8
Abbkine Cell Counting Kit-8（CCK-8）設計用於檢測細胞增殖和細胞毒性。
細胞計數試劑盒-8（CCK-8）使用高度水溶性的四唑鹽WST-8：[2-（2-甲氧基-4-硝基苯基）-3-（4-硝基苯基）-5-（2,4-二磺基苯基 ）-2H-四唑鎓單鈉鹽]通過還原轉化為水溶性橙色甲dye染料。 CCK-8具有低毒性，並且允許用於測定細胞增殖和細胞毒性測定中活細胞數量的靈敏比色測定。 細胞中脫氫酶產生的甲dye染料的量與活細胞的數量成正比。Cell Counting Kit-8（CCK-8）用於檢測細胞增殖(cell proliferation)及細胞毒性(cell toxicity)，其原理基於WST-8經細胞中的脫氫酶降低，得到橙色產物（formazan）。細胞中formazan的含量與活細胞數成正比。細胞計數試劑盒8（Cell Counting Kit-8, CCK-8）通過使用高度水溶性的四唑鹽-WST-8進行非常方便的測定。[2-（2-甲氧基-4-硝基苯基）-3-（4-硝基苯基）-5- （2,4-二磺基苯基）-2H-四唑鎓鹽]在電子介體的存在下還原生成水溶性formazan dye染料。 CCK-8是非放射性的，可進行敏感的比色測定，以測定細胞增殖(cell proliferation)和細胞毒性測定中的活細胞數量。 WST-8被細胞中的脫氫酶(dehydrogenases)還原，得到橙色產物(formazan)，可溶於組織培養基。細胞中脫氫酶(dehydrogenases) 產生的formazan dye染料的量與活細胞的數量成正比。WST-8生產的產品(formazan)是水溶性的，不需要有機溶劑或同位素。而且formazan是穩定和安全的。使用CCK-8的檢測靈敏度高於使用其他四唑鹽（tetrazolium salts, 如MTT，XTT，MTS或WST-1）的檢測靈敏度。
Cell Counting Kit-8 (CCK-8) allows very convenient assays by utilizing highly water-soluble tetrazolium salt-WST-8.[2-(2- methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt] produces a water-soluble formazan dye upon reduction in the presence of an electron mediator. CCK-8, being nonradioactive, allows sensitive colorimetric assays for the determination of the number of viable cells in cell proliferation and cytotoxicity assays. WST-8 is reduced by dehydrogenases in cells to give an orange colored product (formazan), which is soluble in the tissue culture medium. The amount of the formazan dye generated by dehydrogenases in cells is directly proportional to the number of living cells. The product (formazan) produced by WST-8 is water soluble, no organic solvents or isotopes required. And the formazan is stable and safe. The detection sensitivity using CCK-8 is higher than assays using other tetrazolium salts such as MTT, XTT, MTS or WST-1.
Fig. Abbkine Economical Cell Counting Kit-8 (CCK-8) is designed to detect cell proliferation and cell toxicity.
CCK-8; WST-8; CCK8; WST8
| Product name | Cell Counting Kit-8 (CCK-8) |
| Alternative | CCK-8; WST-8; CCK8; WST8 |
| Formulation | Liquid solution |
| Kit components | • CCK-8 solution |
| Features & Benefits | • One-bottle, ready-to-use solution. • The product (formazan) produced by WST-8 is water soluble, No organic solvents or isotopes required. And the formazan is stable and safe. • The detection sensitivity using CCK-8 is higher than assays using other tetrazolium salts such as MTT, XTT, MTS or WST-1. • The same cells can be used for other cell assays because of the low toxicity of CCK-8. |
| Product name | Cell Counting Kit-8 (CCK-8) |
| Alternative | CCK-8; WST-8; CCK8; WST8 |
| Formulation | Liquid solution |
| Kit components | • CCK-8 solution |
| Features & Benefits | • One-bottle, ready-to-use solution. • The product (formazan) produced by WST-8 is water soluble, No organic solvents or isotopes required. And the formazan is stable and safe. • The detection sensitivity using CCK-8 is higher than assays using other tetrazolium salts such as MTT, XTT, MTS or WST-1. • The same cells can be used for other cell assays because of the low toxicity of CCK-8. |
| ackground | Cell Counting Kit-8 (CCK-8) allows very convenient assays by utilizing highly water-soluble tetrazolium salt-WST-8.[2-(2- methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt] produces a water-soluble formazan dye upon reduction in the presence of an electron mediator. CCK-8, being nonradioactive, allows sensitive colorimetric assays for the determination of the number of viable cells in cell proliferation and cytotoxicity assays. WST-8 is reduced by dehydrogenases in cells to give an orange colored product (formazan), which is soluble in the tissue culture medium. The amount of the formazan dye generated by dehydrogenases in cells is directly proportional to the number of living cells. The product (formazan) produced by WST-8 is water soluble, no organic solvents or isotopes required. And the formazan is stable and safe. The detection sensitivity using CCK-8 is higher than assays using other tetrazolium salts such as MTT, XTT, MTS or WST-1. |
| Alternative | CCK-8; WST-8; CCK8; WST8 |
Cell Number Determination Protocol
1. 將細胞懸浮液（100 μl/孔）接種於 96 孔板中。將板在加濕培養箱中預先培養（例如，在 37°C、5% 二氧化碳）。
2. 將 10 μl CCK-8 溶液加入板的每個孔中。小心不要向孔中引入氣泡，因為它們干擾 OD 讀數。
3. 將板在培養箱中培養 1 – 4 小時。孵育時間取決於實驗條件，例如細胞類型和細胞密度；
4. 使用酶標儀測量 450 nm 處的吸光度。為了稍後測量吸光度，在每個孔中加入 10 μl 1% w/v SDS 或 0.1 M HCl，蓋上板並用室溫下避光。 24 小時內不應觀察到吸光度變化
細胞增殖和細胞毒性測定(Cell Proliferation and Cytotoxicity Assay Protocol)
1. 將 100 μl 細胞懸浮液（5000 個細胞/孔）分裝到 96 孔板中。將板在濕化環境下預孵育 24 小時培養箱（例如，37°C，5% CO2）。
2. 將1-10μl不同濃度的待測物質加入板中。
3. 將板在培養箱中培養適當的時間（例如，6、12、24 或 48 小時）。
4. 在板的每個孔中加入 10 μl CCK-8 溶液。小心不要將氣泡引入孔中，因為它們會干擾與 OD 讀數。
5. 將板在培養箱中培養 1 – 4 小時。孵育時間取決於實驗條件，例如細胞類型和細胞密度；
6. 使用酶標儀測量 450 nm 處的吸光度。為了稍後測量吸光度，在每個孔中加入 10 μl 1% w/v SDS 或 0.1 M HCl，蓋上板並用室溫下避光。 24 小時內不應觀察到吸光度變化
商品需用儀器測量 酵素免疫分析儀請按此 (標配405、450、492、630 nm, 可擴增至7個濾片) 或以分光光度計測量 (微量Cuvette)
