TraKine™粒線體染色試劑盒（綠色螢光） | 貨號KTC4003

TraKine™ Mitochondrion Staining Kit (Green Fluorescence)

Fig. Hela cells stained with TraKine™ Mitochondrion Staining Kit (Green Fluorescence).

TraKine™粒腺體染色試劑盒(Mitochondrion Staining Kits, KTC4003)是一套螢光成像工具，可標記活細胞的粒線體(mitochondria)。該試劑盒使用專有的粒線體綠色螢光探針（mitochondrial green fluorescent probe,  Ex / Em = 490/523 nm），與其他探針不同，該探針似乎定位於粒線體(mitochondria)，取決於粒線體膜電位(mitochondrial membrane potential)的情況要少得多。該染料是疏水性化合物(hydrophobic compound)，具有額外的優勢，即它在水溶液中基本不發螢光，並且僅在粒線體的脂質(lipid environment)環境中積累後才發螢光，並且可以染色活細胞(live cells)和甲醛(formaldehyde-fixed cells)固定的細胞，但在滲透(permeabilized)後，螢光信號將丟失。可以使用螢光成像，高含量成像，微板螢光法(microplate fluorometry)或流式細胞術(flow cytometry)來測量螢光。該試劑盒適用於增殖(proliferating)和非增殖細胞(non-proliferating cells)，可用於懸浮細胞(suspension)和貼壁細胞(adherent cells)。

• Kit components
• MitoGreen™ (1000×)
  • Assay Buffer (10×)
• Features & Benefits
• Enables the uniform staining of mitochondrion across a wide variety of mammalian cell types, and can be used for both living and fixed suspended or attached cells.
  • Optimized for various fluorescence platforms such as microplate assays, flow cytometry and fluorescence microscope.
  • Proprietary MitoGreen™ (Ex/Em = 490/523 nm)-much less depending on mitochondrial membrane potential.
• Storage instructions

Refer to list of materials supplied for storage conditions of individual components. Stable for at least 6 months at recommended temperature from date of shipment.

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