CheKine™乳酸檢測試劑盒 | CheKine™ Lactate Assay Kit貨號KTB1100

偵測範圍: 0.03 mM-2 mM

乳酸（CH 3 CH（OH）COO-）在許多生物過程中起重要作用。在增殖細胞中產生無氧運動等條件。異常高濃度的乳酸(Lactate )與諸如癌症、糖尿病( diabetes )和乳酸酸中毒(lactate acidosis)等病理狀況有關。L（+） – 乳酸鹽(L(+)-Lactate)是在人類中間代謝中形成的乳酸鹽之主要立體異構體 (stereo-isomer )。，並且在血液中的含量約為1-2 mmol / L。 D（-）-乳酸也可以在血液，但僅約佔L（+）-乳酸濃度的1-5％。操作手冊 CheKine™ Lactate Assay Kit

Fig. Standard Curve of Lactate assay. The y-axis is OD450 and the x-axis is Lactate concentration (mM).

產品描述：CheKine™乳酸檢測試劑盒(CheKine™ Lactate Assay Kit, KTB1100)為檢測生物樣品（例如血清或血漿，細胞，培養基和發酵培養基）中的L-（+）-乳酸提供了便利的方法。在該試劑盒中，乳酸被乳酸脫氫酶(lactate dehydrogenase)氧化，生成與四唑鹽(tetrazolium salt , WST-8)染料相互作用的產物，形成比色（450 nm）產物，與存在的乳酸(lactate)成比例。偵測範圍: 0.03 mM-2 mM 。可應用於以下樣品來源: serum or plasma, cells, culture and fermentation media

注意事項:

細胞或組織提取物中的NADH或NADPH產生乳酸的背景分析。要去除NADH或NADPH背景，可以將相同量的樣品在沒有乳酸脫氫酶的條件下進行測試。然後可以將背景讀數從乳酸讀數中減去。內源性乳酸脫氫酶（LDH）可能降解乳酸。樣品含LDH（例如培養基或組織裂解液）應通過10 KDa MW過濾旋轉過濾器除去所有蛋白質，然後保持在-80°C進行儲存。

• Product name

CheKine™ Lactate Assay Kit

• Application notes

CheKine™ Lactate Assay Kit provides a convenient means for detecting L-(+)-Lactate in biological samples such as in serum or plasma, cells, culture and fermentation media. In this kit lactate is oxidized by lactate dehydrogenase to generate a product which interacts with a tetrazolium salt WST-8 dye to form a colorimetric (450 nm) product, proportional to the lactate present.

• Kit components
• Lactate Assay Buffer
  • Lactate Dehydrogenase
  • Lactate Dehydrogenase Cofactor
  • WST-8
  • Enhancer
  • L(+)-Lactate Standard (100 mM)
• Features & Benefits
• Determination of L-(+)-Lactate in serum, plasma, cell/tissue samples, urine and other biological fluids.
  • Detect biological samples at a level of 0.03 mM-2 mM.
  • Pre-treatment or purification of samples are not required.
  • Using WST-8 as substrate, more sensitive than MTT.
• Usage notes
• If not assayed immediately, samples can be stored at -80°C for one month.
  • It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.
  • Fresh samples are necessary for good results. If don’t perform the assay at the same time, it’s better to complete the Sample Preparation step before storing the samples.
• Storage instructions

Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.

• Shipping

Gel pack with blue ice.

引用文獻

Experimental evidence and network pharmacology identify the molecular targets of Tong Sheng tablets in cerebral ischemia reperfusion injury

Tuo Liu, Kangqiang Yang, Guoshun Li, et al. Am J Transl Res 2019;11(6):3301-3316.