LDH細胞毒性測定試劑盒| LDH Cytotoxicity Assay Kit貨號KTA1030

LDH Cytotoxicity Assay Kit

Detect lysis of 1000-100,0000 cells per well

LDH (Lactate dehydrogenase, LDH)測定，也稱為 LDH 釋放測定，是一種細胞死亡(cell death)/細胞毒性測定(cytotoxicity assay)，用於評估細胞群中質膜損傷(plasma membrane damage)的水平。乳酸脫氫酶 (Lactate dehydrogenase, LDH)是一種穩定的酶，存在於所有細胞類型中，在質膜((plasma membrane )受損後迅速釋放到細胞培養基中。 LDH 是用於運行細胞毒性(cytotoxicity assay)測定的最廣泛使用的標記物。Detect lysis of 10,00-100,0000 cells per well.

產品描述：Abbkine LDH細胞毒性測定試劑盒 (Abbkine LDH Cytotoxicity Assay Kit, KTA1030)為研究細胞毒性提供了一種簡便的比色測定法。該測定基於在NADH偶聯的酶促反應中四唑鹽MTT的還原成MTT的還原形式，該MTT在565nm處表現出最大吸收。產生的顏色的強度與裂解的細胞數直接相關。在典型的細胞毒性測定中，將靶細胞與細胞毒性化學試劑或細胞毒性細胞（NK細胞，細胞毒性T細胞）(NK cell, cytotoxic T cells)一起培養，以誘導靶細胞死亡( cell death)和LDH釋放。將含有LDH的上清液轉移至新的96孔測定板的孔中，並與LDH反應溶液混合。在室溫下孵育30分鐘後，使用酶標儀讀取565nm（A565）的吸光度。Abbkine LDH Cytotoxicity Assay Kit provides a simple and easy colorimetric assay for the study of cytotoxicity. This assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The intensity of the generated color correlates directly with the cell number lysed. In a typical cytotoxicity assay, target cells are cultured with a cytotoxic chemical agent or a cytotoxic cell (NK cell, cytotoxic T cells) to induce target cell death and LDH release. The LDH-containing supernatants are transferred to wells of a new 96-well assay plate and mixed with the LDH Reaction solution. After an incubation of 30 minutes at room temperature, the absorbance at 565nm (A565) is read using a plate reader.

• Kit components• Assay Buffer
  • Lactic Acid Solution
  • MTT Solution
  • PES Solution
  • LDH Positive Control
  • NAD+ Solution
  • Triton X-100 (10%)
• Features & Benefits• Measures cell death in response to chemical compounds or environmental factors.
  • LDH release from the cytosol into the medium catalyzes a color-forming reaction.
  • Detect lysis of 10,00-100,0000 cells per well.
• Usage notesPerforming an initial titration experiment to determine the optimal number of cells per well of the target cell you plan to use.
• Storage instructionsStore at -20°C and Keep in dark. The Kit has a storage time of 6 months from receipt

 活細胞和死細胞雙染色套組  |  CCK-8細胞增殖和細胞毒性試劑盒  | LDH細胞毒性測定試劑盒 | 細胞衰老檢測試劑盒（β-半乳糖苷酶 | 細胞增殖EdU Image試劑盒 

Catalog no	product name
KTC011001	Cell Counting Kit-8 (CCK-8)
KTA0001	Annexin V-AbFluor™ 405 Apoptosis Detection Kit
KTA0002	Annexin V-AbFluor™ 488 Apoptosis Detection Kit
KTA0003	Annexin V-AbFluor™ 555 Apoptosis Detection Kit
KTA0004	Annexin V-AbFluor™ 647 Apoptosis Detection Kit
KTA1001	Live and Dead Cell Double Staining Kit
KTA1002	Live Cell Tracking Kit (Green Fluorescence)
KTA4001	Mitochondrial Membrane Potential Assay Kit (JC-1)
KTA1030	LDH Cytotoxicity Assay Kit
KTA2010	TUNEL Apoptosis Detection Kit (Green Fluorescence)
KTA2011	TUNEL Apoptosis Detection Kit (Orange Fluorescence)
KTA2020	Cell Cycle Staining Kit
KTA2030	Cell Proliferation EdU Image Kit (Green Fluorescence)
KTA2031	Cell Proliferation EdU Image Kit (Orange Fluorescence)
KTA3020	Caspase-1 Assay Kit (Colorimetric)
KTA3021	Caspase-2 Assay Kit (Colorimetric)
KTA3022	Caspase-3 Assay Kit (Colorimetric)
KTA3023	Caspase-4 Assay Kit (Colorimetric)
KTA3024	Caspase-6 Assay Kit (Colorimetric)
KTA3025	Caspase-8 Assay Kit (Colorimetric)
KTA3026	Caspase-9 Assay Kit (Colorimetric)
KTA1020	Economical Cell Counting Kit-8 (CCK-8)