Annexin V-AbFluor™488細胞凋亡檢測 | 貨號KTA0002
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Annexin V-AbFluor™488細胞凋亡檢測試劑盒 | 貨號KTA0002
細胞凋亡(Apoptosis)是程序性細胞死亡(programmed cell death) 的一種形式，可以從組織中去除不需要的，受損的或衰老的細胞。在正常細胞中，負磷脂(phospholipids)駐留在細胞膜的內側，而膜的外表面則被不帶電荷的磷脂(uncharged phospholipids)（PS）佔據。細胞進入凋亡後，帶負電的PS從質膜(plasma membrane)的內部向外部leaflet轉運，從而使PS暴露於外部細胞環境。人類抗凝劑(human anticoagulant )-Annexin V是一種35-36 kDa的Ca2 +依賴性磷脂結合蛋白(Ca2+-dependent phospholipid-binding protein)，對PS具有很高的親和力。用螢光團(fluorophore)或生物素(biotin)標記的膜聯蛋白V(Annexin V)可以通過與暴露在外部小葉上的PS結合來鑑定凋亡細胞(apoptotic cells)。碘化丙啶（PI）是一種不透過膜的DNA結合染料(membrane-impermeant DNA-binding dye)，通常用於選擇性染色細胞群中的死細胞。PI被活細胞和早期凋亡細胞排除，但會對壞死和晚期凋亡細胞染色。 PI可以被488、532或546 n激發，並發出紅色螢光。
Fig.1. Hela cells were induced with camptothecin for 24 hours and stained with Annexin V- AbFluor™ 488 Apoptosis Detection Kit. The cell is a late stage apoptotic/necrotic cell with both Annexin V- AbFluor™ 488 and PI staining (green membrane with red fragmented nucleus).
Fig.2. Hela cells were induced with camptothecin for 24 hours and stained with Annexin V- AbFluor™ 488 Apoptosis Detection Kit. The combination of AbFluor™ 488 and propidium iodide allows for the distinction between early apoptotic cells (Annexin V- AbFluor™ 488 positive), late apoptotic and/or necrotic cells (Annexin V- AbFluor™ 488 and propidium iodide positive), and viable cells (unstained).
產品描述： Abbkine細胞凋亡檢測試劑盒 (Annexin V-AbFluor™488, 貨號KTA0002) 包含標有Abbkine專有綠色螢光染料的Annexin V-AbFluor™488，可通過流式細胞儀(flow cytometry)或螢光顯微鏡(fluorescence microscopy)對凋亡細胞(apoptotic cells)進行鑑定和定量。用AbFluor™488和碘化丙啶（propidium iodide , PI）對細胞進行同時染色可以區分完整細胞(intact cells)，早期凋亡細胞(early apoptotic)和晚期凋亡細胞(late apoptotic)或壞死細胞(necrotic cells)。
| Product name | Annexin V-AbFluor™ 488 Apoptosis Detection kit (Green Fluorescence) |
| Applications notes | Abbkine Annexin V-AbFluor™ 488 Apoptosis Detection Kit contains Annexin V labeled with Abbkine proprietary green fluorescent dye AbFluor™ 488, which allows the identification and quantitation of apoptotic cells by flow cytometry or fluorescence microscopy. Simultaneous staining of cells with AbFluor™ 488 and propidium iodide (PI) allows the discrimination of intact cells, early apoptotic and late apoptotic or necrotic cells. |
| Alternative | Annexin 5; Annexin V; AbFluor™ 488 |
| Kit components | • Annexin V Binding Buffer (5x) • Annexin V- AbFluor™ 488 • Propidium Iodide (PI) |
| Features & Benefits | • Abbkine AbFluor™ 488 dye is superior to FITC as it is brighter, not affected by pH, and has much better photostability • Annexin V- AbFluor™ 488: Abs/Em = 491/517 nm; PI: Abs/Em = 535/617 nm (with DNA) • Suitable for flow cytometry or fluorescence microscopy |
| Storage instructions | Stable for at least 6 months at 4°C from date of shipment. Protect from light and do not freeze. |
| Shipping | Blue ice |
| Background | Apoptosis is a form of programmed cell death to remove unwanted, damaged, or senescent cells from tissues. In normal cells, the negative phospholipids reside on the inner side of the cellular membrane while the outer surface of the membrane is occupied by uncharged phospholipids (PS). After a cell has entered apoptosis, the negatively charged PS are transported from the inner to the outer leaflet of the plasma membrane, thus exposing PS to the external cellular environment. The human anticoagulant, Annexin V, is a 35–36 kDa Ca2+-dependent phospholipid-binding protein that has a high affinity for PS. Annexin V labeled with a fluorophore or biotin can identify apoptotic cells by binding to PS exposed on the outer leaflet. Propidium iodide (PI) is a membrane-impermeant DNA-binding dye that is commonly used to selectively stain dead cells in a cell population. PI is excluded by live cells and early apoptotic cells, but stains necrotic and late apoptotic cells with compromised membrane integrity. PI can be excited by the 488, 532, or 546 nm laser lines, and emits red fluorescence. |
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