細胞衰老檢測試劑盒（β-半乳糖苷酶） | Cell Senescence β-Galactosidase Staining Kit 貨號KTA3030
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細胞衰老檢測試劑盒（β-半乳糖苷酶） | 貨號KTA3030
Cell Senescence Kit (β-Galactosidase Assay) Cell Senescence; β-Galactosidase Staining Kit
(文獻引用資料出自) Figure 6. Effect of the fractions of fermented turmeric milk extract on SA-β-gal activity in UVA-irradiated Hs68 cells. (A) Microscopy images of senescent Hs68 cells shown by SA-β-gal staining (400× magnification). (B) SA-β-gal activity was quantified by the percentage of SA-β-gal-positive cells in total Hs68 cells. Superscripts indicate significant differences in one-way analysis of variance (ANOVA) and Tukey’s multiple range test (p < 0.05). SA-β-gal: senescence-associated β-galactosidase. NAC: N-acetylcysteine group. Hex100: 100 ppm of hexane fraction of fermented turmeric milk extract. EA100: 100 ppm of ethyl acetate fraction of fermented turmeric milk extract. Bu100: 100 ppm of butanol fraction of fermented turmeric milk extract. WA: 100 ppm of water residue fraction of fermented turmeric milk extract.
細胞衰老(Cellular senescence)是正常細胞停止分裂(cell divide)的現象。衰老的細胞(senescent cells)不再能夠複製(replicate)，但它們仍保持代謝活性(metabolically active)，但因阻滯於G1期，失去了對有絲分裂原的反應能力和合成DNA的能力，不能進入S期。並且與衰老相關的β-半乳糖苷酶活性染色(senescence-associated beta-galactosidase activity)呈陽性，這被認為是細胞衰老的生物標記(biomarker)。細胞的衰老(Cellular senescence)可以通過激活癌基因(oncogenes)和細胞-細胞融合(cell-cell fusion)來實現，DNA對活性氧（reactive oxygen species , ROS）升高的反應會導致DNA損傷(DNA damage)，而不是僅僅依賴於細胞分裂的數目。在正常衰老過程(normal aging)中，組織中的衰老細胞數量顯著增加。
細胞是生物結構(biological structure)和功能(function)的基本單位，也是生物衰老(biological aging)的基本單位。細胞衰老(Cellular senescence)在形態上表現為細胞結構(cell structure)的退化，例如核膜凹陷(nuclear membrane depression)，最終導致核膜塌陷(nuclear membrane collapse)，染色質結構(chromatin structure)改變，超二倍體(hyperdiploid)和異常多倍體細胞(polyploid cells)數量增加。細胞膜的脆性(cell membrane fragility)增加。通透性(permeability)降低，膜受體(membrane receptors)的類型和數量以及對配體(ligands)的敏感性發生變化；脂褐素(lipofuscin)在細胞中積累，許多細胞器和細胞內結構發生變性。細胞衰老(Cell senescence)在生理上表現(physiologically manifested)為功能下降和新陳代謝低下，例如細胞週期停滯(cell cycle arrest)，細胞複製能力 (cell replication ability) 喪失，對促有絲分裂刺激(mitogenic stimulation)的反應性減弱以及對促凋亡因子(pro-apoptotic factors)的反應性改變。細胞內酶活性中心被氧化，酶活性(Enzyme activity)降低，蛋白質合成(protein synthesis)降低等。衰老細胞(senescent cells)不再複制，但它們仍保持代謝活性(metabolically active)，並且衰老相關的β-半乳糖苷酶活性(beta-galactosidase activity)呈陽性，這被認為是細胞衰老生物標記(biomarker of cellular senescence)。
Abbkine細胞衰老檢測試劑盒（Senescence β-Galactosidase Staining Kit, KTA3030）提供了基於衰老過程中pH值為6的衰老相關β-半乳糖苷酶（SA-β-Gal）活性上調的試劑，用於檢測衰老細胞(senescent cells)或組織(tissues)。細胞或組織的老化可以在普通的光學顯微鏡下觀察。 SA-β-Gal僅存在於衰老細胞(senescent cells)中，在衰老前，靜止，腫瘤或永生細胞中無法檢測到Senescence β-Galactosidase Staining Kit provide reagents for detecting the senescent cells or tissues based on the up-regulation of senescence-associated β-galactosidase (SA-β-Gal) activity at pH 6 during aging. The aging of cells or tissues can be observed under a common optical microscope. SA-β-Gal is present only in senescent cells and is undetectable in presenescent, quiescent, tumor or immortal cells
Kit components
10×Fixation Buffer • 10×PBS • Reagent A • Reagent B • Reagent C • X-Gal solution
Features & Benefits
Simple and optimized procedure. • Fast and convenient. • Suitable for aging detection of cultured cells and tissue sections. • Cell senescence β-galactosidase staining kit only stains senescent cells, and does not stain pre-senescence cells (senescent cells), quiescent cells (static cells), immortal cells (immortal cells) or tumor cells.
Storage instructions
Refer to list of materials supplied for storage conditions of individual components. Stable for at least 12 months at recommended temperature from date of shipment.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
活細胞和死細胞雙染色套組 | CCK-8細胞增殖和細胞毒性試劑盒 | LDH細胞毒性測定試劑盒 | 細胞衰老檢測試劑盒（β-半乳糖苷酶 | 細胞增殖EdU Image試劑盒
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