IGF-I重組蛋白 | 貨號PRP100153

Human IGF1 protein

IGF I，也稱為機械生長因子(mechano growth factor)，生長激素-C(somatomedin-C)，IGF-I和IGF1，是一種分泌的蛋白質(secreted protein)，屬於胰島素家族(insulin family)。IGF或somatomedins構成一類多肽，在青春期前的哺乳動物生長中具有關鍵作用。 IGF-I的表達受GH調節並介導產後生長，而IGF-II似乎是由胎盤催乳素在產前發育過程中誘導的。 IGF1 / IGF-1可能是成骨細胞中[1-14C] -2-脫氧D-葡萄糖（2DG）轉運和糖原合成的生理調節劑。 IGF1 / IGF-I刺激大鼠骨骼來源的成骨細胞（rat bone-derived osteoblastic , PyMS）中的葡萄糖轉運，並且在比胰島素低得多的濃度下有效，不僅在糖原和DNA合成方面，而且在提高葡萄糖吸收方面。 IGF1 / IGF-I的缺陷是胰島素樣生長因子I缺乏症（IGF1 deficiency , IGF1缺乏症）的原因，胰島素樣生長因子I缺乏症是一種常染色體隱性遺傳疾病，其特徵在於生長發育遲緩 (growth retardation)，感覺神經性聾(sensorineural deafness) 和智力低下(mental retardation)。

Product name

Human IGF1 protein

• Sequence

Amino acid sequence derived from mature form of human IGF1 isoform 1 (P05019-1) (Gly 49-Ala 118) was expressed and purified.

• Activity

1. Measured by its ability to bind human IGFBP4-His (Cat:10967-H08H) in a functional ELISA.2. Measured by its ability to bind mouse IGFBP4-His (Cat:50250-M08H) in a functional ELISA.3. Measured by its ability to bind Cynomolgus IGFBP1-His (Cat:90085-C08H) in a functional ELISA.4. Measured by its ability to bind human IGF1R (aa 954-1367)-GST (Cat:10164-H20B1) in a functional ELISA.5. Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is typically 0.5-2 ng/mL.6. Measured in a cell proliferation assay using MCF-7 cells. The ED50 for this effect is typically 1-4 ng/mL.

• Protein length

The recombinant mature form of human IGF1 consisting of 71 amino acids and has a calculated molecular mass of 7.8KDa.

• Preparation method

1. coli

• Purity

> 95 % as determined by SDS-PAGE

• Formulation

Lyophilized from sterile 30% Acetonitrile, 0.1% TFA

• Molecular weight

7.8kDa

• Usage notes

Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IGF1 protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

• Storage instructions

Lyophilized Human IGF1 protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

• Shipping

The product is shipped at ambient temperature

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貨號	產品名稱	胞器染色位置	螢光波段
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貨號KTC4100	Pro活細胞微管蛋白染色	微管蛋白(Tubulin)	Ex/Em = 500/520 nm	活細胞或固定之細胞
貨號KTC4001	Abbkine 細胞膜染色	細胞膜 (plasma membrane)	Ex/Em = 484/501 nm	活細胞、固定懸浮、貼附細胞
貨號BMD00063	DAPI Staining Solution	細胞核 dsDNA	Ex/Em: 358/461 nm (with DNA)	染色活細胞和固定細胞
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