細胞增殖試驗套組(EdU100個反應/CCK8-2000個反應) | Cell Proliferation Assay Cocktail貨號KTD103-EN

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細胞增殖試驗套組(EdU100個反應/CCK8-2000個反應) | Cell Proliferation Assay Cocktail貨號KTD103-EN

Cell Proliferation Assay Cocktail不僅可以直接從DNA水平準確檢測細胞增殖狀態(cell proliferation status)，還可以通過細胞代謝(cell metabolism)水平實現對細胞增殖狀態的高通量(high-throughput)檢測。是一種通用的細胞增殖檢測產品(universal cell proliferation detection product)。細胞增殖(Cell proliferation)是通過細胞分裂(cell division)來增加細胞數量的過程，會產生許多重要的變化，包括DNA的合成(synthesis)、細胞代謝(cell metabolism)的增加、增殖特異性蛋白(proliferation-specific proteins)的表達等。Cell Proliferation Assay Cocktail， (KTB1030-EU)。包括有兩種商品的組合: EdU Cell Proliferation Image Kit (Green Fluorescence)-100T。為BrdU分析新型替代方法，可偵測 DNA合成/細胞增殖檢測。Cell Counting Kit-8（CCK-8）用於檢測細胞增殖(cell proliferation)及細胞毒性(cell toxicity)。

Fig.1. HEK293 and L929 cells in the positive control group were induced by cytotoxicity inducer for 30 min, while the control group was not given any treatment. Enhanced Cell Counting Kit-8 (CCK-8) was used to detect the cytotoxicity of HEK293 and L929 cells after cytotoxicity induction.

Fig.2. Cell Proliferation EdU Image Kit (Green Fluorescence) was used to detect the cytotoxicity of HEK293 cells after cytotoxicity induction.

Product name	Cell Proliferation Assay Cocktail
Applications notes	Cell Proliferation Assay Cocktail can not only accurately detect cell proliferation status directly from DNA level, but also realize high-throughput detection of cell proliferation status through cell metabolism level. It is a universal cell proliferation detection product.
Kit components	•EdU Cell Proliferation Image Kit (Green Fluorescence)-100T EdU (10 mM) BSA Wash Solution (5×) AbFluor 488 azide 10×Reaction buffer Copper Reagent Reducing Agent DAPI (500×) •Cell Counting Kit-8 (CCK-8)-2000T CCK-8 •Postive Control-100T Postive Control
Features & Benefits	• Standardized: The patented cytotoxicity positive reference substance can perfectly solve the problem of non-standardization of proliferation/toxicity test results at present. • Universal: It can detect cell proliferation status most directly and accurately, and it can be also chosen as high-throughput detection detection, which covers most cell proliferation/toxicity detection needs.
Storage instructions	Refer to list of materials supplied for storage conditions of individual components.
Shipping	Blue ice
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Cell proliferation is a process of increasing the number of cells by means of cell division, which will produce many important changes, including the synthesis of DNA, the increase of cell metabolism, the expression of proliferation-specific proteins and so on.

活細胞和死細胞雙染色套組 | CCK-8細胞增殖和細胞毒性試劑盒 | LDH細胞毒性測定試劑盒 | 細胞衰老檢測試劑盒（β-半乳糖苷酶 | 細胞增殖EdU Image試劑盒

產品貨號	產品品項	反應	測量儀器
貨號KTA1001	活細胞和死細胞雙染色試劑盒	100個反應	活細胞綠色螢光染劑Ex/Em = 488/530 nm; 死細胞紅色螢光染料(Ex/Em = 535/617)
貨號 KTA2010	TUNEL細胞凋亡檢測試劑盒	50個反應	螢光酶標儀、螢光顯微鏡或流式細胞儀。FITC通道（Ex / Em = 490nm / 520 nm）
貨號 KTC011001	CCK-8細胞增殖和細胞毒性試劑盒	500個反應	吸收光450nm
貨號KTC4003	TraKine™粒線體染色	50個反應/250個反應	Ex / Em = 490/523 nm增殖(proliferating)和非增殖細胞(non-proliferating cells)
貨號KTC4100	Pro活細胞微管蛋白染色	50個反應/250個反應	Ex/Em = 500/520 nm
貨號KTC4001	Abbkine 細胞膜染色	100個反應/500個反應	Ex/Em = 484/501 nm
貨號BMD00063	DAPI Staining Solution	10MG/100MG	Ex/Em: 358/461 nm (with DNA)
貨號KTC4210	活細胞Lysosome染色	50個反應/250個反應	deep red fluorescent dye , Ex/Em:650/665nm
貨號KTA3030	細胞衰老檢測試劑盒（β-半乳糖苷酶）	100個反應	光學顯微鏡
貨號KTA1020	CCK-8細胞增殖和細胞毒性試劑盒	1000個反應/10,000個反應	吸收光450nm
貨號KTA2030	細胞增殖EdU Image試劑盒 (BrdU替代法)	100個反應/500個反應	AbFluor 488 azide (Ex/Em = 501/525 nm)
貨號KTB1400	一氧化Dan（NO）檢測試劑盒	96個反應/ 192個反應	colorimetric (540 nm) 檢測範圍：1-100 µM
貨號KTB1070	CheKine™黃嘌呤氧化酶檢測	96個反應/ 480個反應	OD 450 nm 線性範圍15.6 -500 mU/
貨號KTD3001	BCA蛋白定量試劑盒	2000個反應/ 5000個反應	OD 562 nm 相容性 5 % SDS, 5 % Triton X-100, 5 % Tween-20, 60, 80 50 to 1000 µg/ml.
貨號KTB1110	乳酸脫氫酶（LDH）測定試劑盒	96個反應/ 480個反應	λmax= 450 nm 1- 20 U/mL of LDH
貨號PRP100153	IGF-I重組蛋白	200UG/1MG	human IGF1 isoform 1 (P05019-1) (Gly 49-Ala 118) > 95 % as determined by SDS-PAGE
貨號 KTA2020	細胞週期染色檢測	100個反應/500個反應	Ex/Em=535/615 nm.
貨號BMC21400	PurKine™內毒素去除預裝離心柱	1ml×5 3mlx5	More than 2,000,000 EU/mL to eliminate >99% of endotoxins.
貨號KTA1200	CheKine™蛋白羰基比色測定試劑盒	48個反應/ 96個反應	吸收光370 nm
貨號KTB1080	超氧陰離子清除測定試劑盒	48個反應/ 96個反應	Superoxide anion scavenging rate D% =（∆∆ODControl – ∆∆ODSample）÷ ∆∆ODControl ×100%
貨號KTB1090	清除氫氧自由基能力測定試劑	48個反應/ 96個反應	吸收光 536nm Hydroxyl radical scavenging rate D% =（ASample – AControl）÷（ABlank – AControl）×100%
貨號KTB2220	TC總膽固醇檢測試劑盒	48個反應/ 96個反應	500nm Standard: 5 μmol/mL
貨號KTB1510	CheKine™尿酸（UA）比色分析試劑盒	48個反應/ 96個反應	吸收光505 nm Standard: 5 μmol/mL.
貨號KTB1300	Glucose葡萄糖測定試劑盒	96個反應/ 192個反應	吸光值630nm 4- 300 mg/dL)
貨號KTB1100	Lactate 乳酸檢測試劑盒	96個反應/ 480個反應	colorimetric (450 nm) 0.03 mM-2 mM
貨號KTB1700	組織和血液鹼性磷酸酶（ Alkaline Phosphatase, AKP / ALP）比色測定	96個反應/ 96 X5盤	吸收光510 nm
貨號KTB1500	總抗氧化劑能力TAC分析	96個反應/ 480個反應	593 nm 偵測範圍: 0.15-3 mM
貨號KTA0002	Annexin V-AbFluor™488細胞凋亡檢測試劑盒	20個反應/50個反應/100個反應	Annexin V- AbFluor™ 488: Abs/Em = 491/517 nm; PI: Abs/Em = 535/617 nm (with DNA)
貨號KTE7013	EliKine™ Rat IL-6 ELISA Kit試劑盒	96個反應/ 96 X5盤	450 nm 偵測範圍: 250pg/mL-16000 pg/mL, 最低靈敏度100 pg/mL
貨號KTE0003	雌二醇（Estradiol , E2）試劑盒	96個反應/ 96 X5盤	450 nm 偵測範圍40 pg/ml-1500 pg/ml
貨號KTE6032	TNF–α Alpha型腫瘤壞死因子 ELISA試劑盒	96個反應/ 96 X5盤	450 nm 7.8 pg/ml-500 pg/ml
貨號KTE6033	VEGF血管內皮生長因子ELISA試劑盒	96個反應/ 96 X5盤	450 nm 偵測範圍: 31.25 pg/mL-2000 pg/mL
貨號KTE7005	白介素-1β（Interleukin-1β, IL-1β）ELISA試劑盒	96個反應/ 96 X5盤	Microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm. 偵測範圍: 15.6 pg/ml-1000 pg/ml
貨號KTE70003	小鼠黃嘌呤脫氫酶/氧化酶（XDH）ELISA試劑盒	96個反應/ 96 X5盤	450 nm 偵測範圍: 22.5 ng/L-360 ng/L, 最低靈敏度1.0 ng/L。
貨號 A02051HRP	His-tag標籤多株抗體-HRP	50ul/200ul	WB (1:5000).
貨號A02170MGB	Anti-V5 Tag Mouse Monoclonal Antibody (11D5), 磁珠	100ul/400ul/2ml	GKPIPNPLLGLDST epitope
貨號KTA4001	JC-1線粒體膜電位測定試劑盒	100個反應/ 500個反應	（聚結的JC-1，Ex / Em = 585/590 nm）發出綠色螢光（JC-1單體，Ex / Em = 510/527 nm） JC-1 Stain CCCP (10 mM) Assay Buffer (5×)
貨號KTB1600	穀胱甘肽(Glutathione)檢測	96個反應	412 nm 2-200 µg/mL
貨號BMD00063	DAPI Staining Solution	1MG/10MG/100MG	λEx/λEm: 358/461 nm (with DNA)
貨號KTP3001	細胞核/細胞質分離試劑盒	50個反應/ 200個反應	Cytoplasmic Solution A (CES A) Cytoplasmic Solution B (CES B) • Nuclear Extraction Solution (NES) DTT (500X) Protease Inhibitor (100X)
貨號BMD00064-50UG	Calcein AM鈣黃綠素活細胞螢光染劑	50ug/1mg/10mg	λEX/λEm: 494/517 nm (pH 8) (calcein)
貨號 KTB1660	硫氧還蛋白過氧化物酶（TPX）檢測	96個反應	240nm
貨號KTB1020	NAD/NADH 分析試劑盒	96個反應/ 480個反應	OD565 nm 96 孔板測定中的檢測限值為 0.78 μM 線性最高 50 μM NAD+/NADH。
貨號KTB1070	CheKine™黃嘌呤氧化酶檢測	96個反應/ 480個反應	OD 450 nm 線性範圍15.6 -500 mU/mL
貨號KTB1040	Catalase過氧化氫酶活性檢測試劑盒	96個反應/ 480個反應	OD 540 nm 2-75 µM
貨號KTB1060	過氧化物酶（Peroxidases）分析試劑盒	96個反應	OD 664 nm 線性範圍為15.6-1000 µU/mL
貨號KTB1050	脂質過氧化檢測試劑盒(MDA)	96個反應	OD 532 nm 1-100 µM/L
貨號KTB1030	超氧化物歧化酶（SOD）活性測定試劑盒	96個反應	200-3.13 U/ml
貨號KTA1030	LDH細胞毒性測定試劑盒	96個反應/ 480個反應	OD 565 nm 10,00-100,0000 cells per well
貨號KTB3010	CheKine™ 花青素還原酶（ANR）活性比色測定試劑盒	48個反應/ 96個反應	absorption at 340 nm
貨號KTB1542	Tannase(TAN) 活性比色測定試劑盒	48個反應/ 96個反應	absorbance at 270 nm 0.3125-20 μmoL/mL
貨號KTB1541	CheKine™ 單寧比色測定試劑盒 \| CheKine™ Tannin Colorimetric Assay Kit	48個反應/ 96個反應	absorbance at 760 nm 0.0156-1 mg/mL
貨號KTB2250	CheKine™ 微量高密度脂蛋白膽固醇 (HDL-C) 檢測試劑盒	48個反應/ 96個反應	Absorption peak at 500nm Detection range: 0.078-5 mmol/L(The Detection range corresponds to the standard, and the actual content of sample is 0.156-10 mmol/L）
貨號KTB2260	CheKine™ 微量低密度脂蛋白膽固醇 (LDL-C) 檢測試劑盒	48個反應/ 96個反應	Absorption peak at 500nm Detection range: 0.078-5 mmol/L(The Detection range corresponds to the standard, and the actual content of sample is 0.117-7.5 mmol/L）
貨號KTB1290	CheKine™ 烏頭酸酶 (ACO) 活性比色測定試劑盒	48個反應/ 96個反應	absorption at 340 nm.
貨號KTB1560	醇醯基轉移酶（AAT）活性比色檢測試劑盒	48個反應/ 96個反應	absorption at 412 nm.
貨號KTB1390	CheKine™ 澱粉分支酶 (SBE) 活性比色測定試劑盒	48個反應/ 96個反應	absorption at 660 nm
貨號PRP1012	Human IL-6 protein重組蛋白	5ug/20ug/100ug/1mg	human IL-6 (NP_000591.1) (Val 30-Met 212)/ E. coli
貨號PRP100273	Human IL-4 protein重組蛋白	20ug/100ug/1mg	ED50 for this effect is 0.05-0.2 ng/mL. The specific activity of Recombinant Human IL-4 is approximately 3.2 × 10E4 IU/μg.
貨號PRP100159	Human EGF protein重組蛋白	100ug/1mg
貨號KTB1041	CheKine™ 過氧化氫 (H2O2) 檢測試劑盒	96個反應/ 480個反應	OD 580 nm : 1 µM-100 µM
貨號KTB1210	CheKine™ 超氧陰離子比色測定試劑盒	48個反應/ 96個反應	absorption peak at 540nm 0.0125-0.5 mg/mL

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