活細胞和死細胞雙染色套組 | Live and Dead Cell Double Staining Kit貨號KTA1001
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活細胞和死細胞雙染色套組 | Live and Dead Cell Double Staining Kit貨號KTA1001
Detect using either microscopy or FACS.
細胞介導細胞毒性(Cell-mediated cytotoxicity)是免疫系統(immune system) 對體內受損細胞，cytolysis的一個重要現象。區分活細胞和死細胞對於研究生長控制和細胞死亡具重要性。Abbkine 活細胞和死細胞雙染色試劑盒 (Abbkine Live and Dead Cell Double Staining Kit) 提供了一個方便的測定,進行評估細胞的可行性；套組提供兩種probes同時測定活細胞和死細胞, 可用於測量細胞健康的識別參數:血漿膜完整性(plasma membrane integrity) 和細胞內酯酶活性(intracellular esterase activity)。該試劑盒利用LiveDye活細胞染劑; 一種細胞滲透的綠色螢光染料(cell-permeable green fluorescent dye , Ex/Em = 488/530 nm)染色活細胞(live cells)。NucleiDye核染料,為一種細胞不可滲透的紅色螢光染料(Ex/Em = 535/617) , 用於染色死細胞(dead cells)。
Abbkine 活細胞和死細胞雙染色試劑盒 (Live and Dead Cell Double Staining Kit, KTA1001) 提供了一個方便的測定評估死活細胞的快速方法,基於同時測定活細胞和死細胞與兩種Probe。測量細胞健康、血漿膜完整性、和細胞內酯酶活性。該試劑盒利用LiveDye一種細胞滲透的綠色螢光染料(Ex/Em = 488/530 nm)染色活細胞和核染料；NucleiDye一種細胞不可滲透的紅色螢光染料(Ex/Em = 535/617)以染色死細胞。
| Product name | Live and Dead Cell Double Staining Kit |
| Applications notes | Abbkine Live and Dead Cell Double Staining Kit provides a convenient assay to evaluate the viability of cells, based on the simultaneous determination of live and dead cells with two probes that measure recognized parameters of cell health: plasma membrane integrity and intracellular esterase activity. The kit utilizes LiveDye, a cell-permeable green fluorescent dye (Ex/Em = 488/530 nm), to stain live cells and NucleiDye, a cell non-permeable red fluorescent dye (Ex/Em = 535/617), to stain dead cells. |
| it components | • LiveDye • NucleiDye • Assay Buffer (10X) |
| Features & Benefits | • Suitable for mammalian cells staining. • Useful for the rapid quantitation of cell viability using flow cytometery or fluorescent microscopy. • LiveDye (green, Ex/Em=488/530nm), NucleiDye (red, Ex/Em= 535/617nm). |
| Usage notes | NucleiDye is a potential mutagen. Use appropriate precautions when handling this reagent. |
| Storage instructions | Refer to list of materials supplied for storage conditions of individual components. Stable for at least 12 months at recommended temperature from date of shipment. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Cell-mediated cytotoxicity is an important phenomenon characterized by cytolysis of a compromised cell in the body by immune system. Distinguishing between live and dead cells is very important for investigation of growth control and cell death. |
1. Ocular surface repair using decellularized porcine conjunctiva
L Zhao, Y Jia, C Zhao. Acta biomaterialia, 2019 – Elsevier
2. Grafting antibacterial polymer brushes from titanium surface via polydopamine chemistry and activators regenerated by electron transfer ATRP
Shuangshuang Wang, Jixuan Song, Yuchao Li, et al. Reactive and Functional Polymers. July 2019, 140: 48-55.
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