α-澱粉酶活性比色測定試劑盒 | α-Amylase Activity Colorimetric Assay Kit貨號KTB1370
α-澱粉酶活性比色測定試劑盒 |α-Amylase Activity Colorimetric Assay Kit貨號KTB1370
偵測範圍0.0156-1 mg/mL
澱粉水解酶(Starch hydrolases),包括α-澱粉酶(α -amylase, α-Al,EC 3.2.1.1)和β-澱粉酶(β -amylase, β-Al)。 α-澱粉酶(α -amylase)隨機催化澱粉中的α-1,4-糖苷鍵(α-1, 4-glycosidic bond)水解(hydrolysis)生成葡萄糖(glucose)、麥芽糖(maltose)、糊精(dextrin)等還原糖(reducing sugars),同時降低澱粉(starch)的粘度(viscosity),故又稱液化酶(liquefaction enzyme)。CheKine™ α-澱粉酶活性比色測定試劑盒(CheKine™ α-Amylase Activity Colorimetric Assay Kit, KTB1370) 為檢測 α-澱粉酶活性(α-Amylase Activity)提供了方便的工具。其原理是澱粉水解酶(starch hydrolase)催化澱粉水解生成還原糖(reducing sugar),還原3, 5-二硝基水楊酸(3, 5-dinitrosalicylic acid)生成棕紅色物質,在540 nm處有吸收峰。通過測量 540 nm 處的吸光度增加率來計算澱粉酶活性(Amylase activity)。
Product name | CheKine™ α-Amylase Activity Colorimetric Assay Kit |
Applications notes | CheKine™ α-Amylase Activity Colorimetric Assay Kit provides a convenient tool for detection of α-Amylase Activity. The principle is that starch hydrolase catalyzes the hydrolysis of starch to produce reducing sugar, which reduces 3, 5-dinitrosalicylic acid to produce brown-red substance with an absorption peak at 540 nm. Amylase activity was calculated by measuring the absorbance increase rate at 540 nm. |
Kit components | •DNS Reagent •Substrate •Standard |
Features & Benefits | • Determination of α-Amylase Activity in a variety of biological samples such as serum, plasma, animal and plant tissues. • Detailed sample preparation and result calculation methods are provided. • Standard and standard curve line are provided. |
Calibration range | 0.0156-1 mg/mL |
Limit of detection | 0.0078 mg/mL |
Storage instructions | Storage at 4°C and protected from light upon receipt. Kit has a storage time of 12 months form receipt. |
Shipping | Blue ice |
Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
Background | Starch hydrolases, including α -amylase (α -Al, EC 3.2.1.1) and β -amylase (β -Al). α -amylase randomly catalyzes the hydrolysis of α-1, 4-glycosidic bond in starch to generate glucose, maltose, maltose, dextrin and other reducing sugars, while reducing the viscosity of starch, so it is also called liquefaction enzyme. |
活細胞和死細胞雙染色套組 | CCK-8細胞增殖和細胞毒性試劑盒 | LDH細胞毒性測定試劑盒 | 細胞衰老檢測試劑盒(β-半乳糖苷酶 | 細胞增殖EdU Image試劑盒
產品貨號 | 品項 | 反應 | 測量儀器 |
貨號KTA1001 | 活細胞和死細胞雙染色試劑盒 | 100個反應 |
活細胞綠色螢光染劑Ex/Em = 488/530 nm; 死細胞紅色螢光染料(Ex/Em = 535/617) |
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50個反應 | 螢光酶標儀、螢光顯微鏡或流式細胞儀。FITC通道(Ex / Em = 490nm / 520 nm) |
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CCK-8細胞增殖和細胞毒性試劑盒 | 500個反應 | 吸收光450nm |
貨號KTC4003 | TraKine™粒線體染色 | 50個反應/250個反應 | Ex / Em = 490/523 nm增殖(proliferating)和非增殖細胞(non-proliferating cells) |
貨號KTC4100 | Pro活細胞微管蛋白染色 | 50個反應/250個反應 | Ex/Em = 500/520 nm |
貨號KTC4001 | Abbkine 細胞膜染色 | 100個反應/500個反應 | Ex/Em = 484/501 nm |
貨號BMD00063 | DAPI Staining Solution | 10MG/100MG | Ex/Em: 358/461 nm (with DNA) |
貨號KTC4210 | 活細胞Lysosome染色 | 50個反應/250個反應 | deep red fluorescent dye , Ex/Em:650/665nm |
貨號KTA3030 | 細胞衰老檢測試劑盒(β-半乳糖苷酶) | 100個反應 | 光學顯微鏡 |
貨號KTA1020 | CCK-8細胞增殖和細胞毒性試劑盒 | 1000個反應/10,000個反應 | 吸收光450nm |
貨號KTA2030 |
(BrdU替代法) |
100個反應/500個反應 | AbFluor 488 azide (Ex/Em = 501/525 nm) |
貨號KTB1400 | 一氧化Dan(NO)檢測試劑盒 |
96個反應/ 192個反應 |
colorimetric (540 nm) 檢測範圍:1-100 µM |
貨號KTB1070 | CheKine™黃嘌呤氧化酶檢測 |
96個反應/ 480個反應 |
OD 450 nm 線性範圍15.6 -500 mU/ |
貨號KTD3001 | BCA蛋白定量試劑盒 |
2000個反應/ 5000個反應 |
OD 562 nm 相容性 5 % SDS, 5 % Triton X-100, 5 % Tween-20, 60, 80 50 to 1000 µg/ml. |
貨號KTB1110 | 乳酸脫氫酶(LDH)測定試劑盒 |
96個反應/ 480個反應 |
λmax= 450 nm 1- 20 U/mL of LDH |
貨號PRP100153 | IGF-I重組蛋白 | 200UG/1MG |
human IGF1 isoform 1 (P05019-1) (Gly 49-Ala 118) > 95 % as determined by SDS-PAGE |
貨號 KTA2020 | 細胞週期染色檢測 | 100個反應/500個反應 | Ex/Em=535/615 nm. |
貨號BMC21400 | PurKine™內毒素去除預裝離心柱 |
1ml×5 3mlx5 |
More than 2,000,000 EU/mL to eliminate >99% of endotoxins. |
貨號KTA1200 | CheKine™蛋白羰基比色測定試劑盒 |
48個反應/ 96個反應 |
吸收光370 nm |
貨號KTB1080 | 超氧陰離子清除測定試劑盒 |
48個反應/ 96個反應 |
Superoxide anion scavenging rate D% =(∆∆ODControl – ∆∆ODSample)÷ ∆∆ODControl ×100% |
貨號KTB1090 | 清除氫氧自由基能力測定試劑 |
48個反應/ 96個反應 |
吸收光 536nm Hydroxyl radical scavenging rate D% =(ASample – AControl)÷(ABlank – AControl)×100% |
貨號KTB2220 | TC總膽固醇檢測試劑盒 |
48個反應/ 96個反應 |
500nm Standard: 5 μmol/mL |
貨號KTB1510 | CheKine™尿酸(UA)比色分析試劑盒 |
48個反應/ 96個反應 |
吸收光505 nm Standard: 5 μmol/mL. |
貨號KTB1300 | Glucose葡萄糖測定試劑盒 |
96個反應/ 192個反應 |
吸光值630nm 4- 300 mg/dL) |
貨號KTB1100 | Lactate 乳酸檢測試劑盒 |
96個反應/ 480個反應 |
colorimetric (450 nm) 0.03 mM-2 mM |
貨號KTB1700 | 組織和血液鹼性磷酸酶( Alkaline Phosphatase, AKP / ALP)比色測定 |
96個反應/ 96 X5盤 |
吸收光510 nm |
貨號KTB1500 | 總抗氧化劑能力TAC分析 |
96個反應/ 480個反應 |
593 nm 偵測範圍: 0.15-3 mM |
貨號KTA0002 | Annexin V-AbFluor™488細胞凋亡檢測試劑盒 | 20個反應/50個反應/100個反應 | Annexin V- AbFluor™ 488: Abs/Em = 491/517 nm; PI: Abs/Em = 535/617 nm (with DNA) |
貨號KTE7013 | EliKine™ Rat IL-6 ELISA Kit試劑盒 |
96個反應/ 96 X5盤 |
450 nm 偵測範圍: 250pg/mL-16000 pg/mL, 最低靈敏度100 pg/mL |
貨號KTE0003 | 雌二醇(Estradiol , E2)試劑盒 |
96個反應/ 96 X5盤 |
450 nm 偵測範圍40 pg/ml-1500 pg/ml |
貨號KTE6032 | TNF–α Alpha型腫瘤壞死因子 ELISA試劑盒 |
96個反應/ 96 X5盤 |
450 nm 7.8 pg/ml-500 pg/ml |
貨號KTE6033 | VEGF血管內皮生長因子ELISA試劑盒 |
96個反應/ 96 X5盤 |
450 nm 偵測範圍: 31.25 pg/mL-2000 pg/mL |
貨號KTE7005 | 白介素-1β(Interleukin-1β, IL-1β)ELISA試劑盒 |
96個反應/ 96 X5盤 |
Microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm. 偵測範圍: 15.6 pg/ml-1000 pg/ml |
貨號KTE70003 | 小鼠黃嘌呤脫氫酶/氧化酶(XDH)ELISA試劑盒 |
96個反應/ 96 X5盤 |
450 nm 偵測範圍: 22.5 ng/L-360 ng/L, 最低靈敏度1.0 ng/L。 |
貨號 A02051HRP | His-tag標籤多株抗體-HRP | 50ul/200ul | WB (1:5000). |
貨號A02170MGB | Anti-V5 Tag Mouse Monoclonal Antibody (11D5), 磁珠 | 100ul/400ul/2ml | GKPIPNPLLGLDST epitope |
貨號KTA4001 | JC-1線粒體膜電位測定試劑盒 |
100個反應/ 500個反應 |
(聚結的JC-1,Ex / Em = 585/590 nm)發出綠色螢光(JC-1單體,Ex / Em = 510/527 nm) JC-1 Stain CCCP (10 mM) Assay Buffer (5×) |
貨號KTB1600 | 穀胱甘肽(Glutathione)檢測 | 96個反應 |
412 nm 2-200 µg/mL |
貨號BMD00063 | DAPI Staining Solution | 1MG/10MG/100MG | λEx/λEm: 358/461 nm (with DNA) |
貨號KTP3001 | 細胞核/細胞質分離試劑盒 |
50個反應/ 200個反應 |
Cytoplasmic Solution A (CES A) Cytoplasmic Solution B (CES B) • Nuclear Extraction Solution (NES) DTT (500X) Protease Inhibitor (100X) |
貨號BMD00064-50UG | Calcein AM鈣黃綠素活細胞螢光染劑 | 50ug/1mg/10mg |
λEX/λEm: 494/517 nm (pH 8) (calcein)
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貨號 KTB1660 | 硫氧還蛋白過氧化物酶(TPX)檢測 | 96個反應 | 240nm |
貨號KTB1020 | NAD/NADH 分析試劑盒 |
96個反應/ 480個反應 |
OD565 nm 96 孔板測定中的檢測限值為 0.78 μM 線性最高 50 μM NAD+/NADH。 |
貨號KTB1070 | CheKine™黃嘌呤氧化酶檢測 |
96個反應/ 480個反應 |
OD 450 nm 線性範圍15.6 -500 mU/mL |
貨號KTB1040 | Catalase過氧化氫酶活性檢測試劑盒 |
96個反應/ 480個反應 |
OD 540 nm 2-75 µM |
貨號KTB1060 | 過氧化物酶(Peroxidases)分析試劑盒 | 96個反應 |
OD 664 nm 線性範圍為15.6-1000 µU/mL |
貨號KTB1050 | 脂質過氧化檢測試劑盒(MDA) | 96個反應 |
OD 532 nm 1-100 µM/L |
貨號KTB1030 | 超氧化物歧化酶(SOD)活性測定試劑盒 | 96個反應 | 200-3.13 U/ml |
貨號KTA1030 | LDH細胞毒性測定試劑盒 |
96個反應/ 480個反應 |
OD 565 nm 10,00-100,0000 cells per well |