Ki 67多株抗體 | 貨號ABP51674
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Ki 67 polyclonal Antibody (Synthesized peptide AA range: 3170-3250)
產品描述： MKI67編碼與細胞增殖cellular proliferation有關並且可能是細胞增殖所必需的核蛋白（增殖Ki-67的標記）。已經描述了剪接的轉錄物變體。 一個相關的假基因pseudogene存在於X染色體上。
活細胞和死細胞雙染色套組 | CCK-8細胞增殖和細胞毒性試劑盒 | LDH細胞毒性測定試劑盒 | 細胞衰老檢測試劑盒（β-半乳糖苷酶 | 細胞增殖EdU Image試劑盒
Fig.1. Western Blot analysis of Hela (1), 293T (2), diluted at 1:1000.
Fig.2. Immunofluorescence analysis of human breast cancer tissue. 1, Ki-67 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.
Fig.3. Immunohistochemical analysis of paraffin-embedded human lung tissue. 1, Ki-67 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
- Product name
Ki-67 Polyclonal Antibody
- Immunogen
Synthesized peptide derived from the C-terminal region of human Ki-67 at AA range: 3170-3250
- Host
Rabbit
- Reactivity
Human
- Applications
ELISA, IF, IHC-P, WB
- Application notes
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:20000). Not yet tested in other applications.
- Clonality
Polyclonal
- Isotype
Rabbit IgG
- Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
- Formulation
Liquid solution
- Concentration
1 mg/ml
- Storage buffer
PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
- Storage instructions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
- Shipping
Gel pack with blue ice.
- Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
- Background
MKI67 encodes Antigen KI-67 that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X.
- Gene ID
- Alternative names
MKI67; Antigen KI-67
- Others
Ki-67 Polyclonal Antibody detects endogenous levels of Ki-67 protein.
- Accession
| 貨號 | 產品名稱 | 胞器染色位置 | 螢光波段 | |
| 貨號KTC4003 | TraKine™粒線體染色 | 粒線體(mitochondria) | Ex / Em = 490/523 nm | 增殖(proliferating)和非增殖細胞(non-proliferating cells) |
| 貨號KTC4100 | Pro活細胞微管蛋白染色 | 微管蛋白(Tubulin) | Ex/Em = 500/520 nm | 活細胞或固定之細胞 |
| 貨號KTC4001 | Abbkine 細胞膜染色 | 細胞膜 (plasma membrane) | Ex/Em = 484/501 nm | 活細胞、固定懸浮、貼附細胞 |
| 貨號BMD00063 | DAPI Staining Solution | 細胞核 dsDNA | Ex/Em: 358/461 nm (with DNA) | 染色活細胞和固定細胞 |
| 貨號KTC4210 | 活細胞Lysosome染色 | Lysosome | deep red fluorescent dye , Ex/Em:650/665nm | 染色活細胞和固定細胞 |