自由基能力測定-清除氫氧自由基能力測定試劑

羥基自由基•OH是氫氧根離子（OH-）的中性形式，具有高反應性（易於變成羥基），因此壽命短。但是，它們是自由基化學的重要組成部分。最明顯的是，羥基自由基是由氫過氧化物（hydroperoxides , ROOH）的分解產生的，或者在大氣化學中是由激發的原子氧(excited atomic oxygen)與水的反應產生的。羥基幾乎可以破壞所有類型的大分子：碳水化合物(carbohydrates)，核酸(nucleic acids)（突變），脂質（lipid peroxidation, 脂質過氧化）和氨基酸（例如Phe轉化為m-酪氨酸(m-Tyrosine)和o-酪氨酸(o-Tyrosine），導致細胞結構(cell structure)和功能受損(function damage)，然後導致新陳代謝失調(metabolic disorders)。羥基自由基的清除能力代表了抗氧化能力，廣泛用於抗氧化保健品(antioxidant health products)和藥物的研究。操作手冊下載

CheKine™ Hydroxyl Free Radical Scavenging Capacity Assay Kit

產品描述：CheKine™羥自由基清除能力測定試劑盒(Hydroxyl Free Radical Scavenging Capacity Assay Kit, KTB1090),為研究各種樣品中的羥自由基清除能力(Hydroxyl radical scavenging Capacity)，提供了一種簡便的比色法。在該測定中，H2O2 / Fe2 +通過Fenton反應產生羥基自由基(hydroxyl radical )，並將Fe2 +氧化為Fe3 +，導致536nm吸光度降低。樣品在536nm吸光度降低時的抑制率可以反映樣品的羥自由基清除能力(Hydroxyl Free Radical Scavenging Capacity)。

Kit components

• Lysis Buffer
  • Assay Buffer
  •Chromogen A
  • Chromogen B
  • H2O2 (0.03%)
• Features & Benefits
• Determination the Hydroxyl Free Radical Scavenging Capacity in various samples, including serum, plasma, tissue lysates and other biological fluids.
• Usage notes
• If not assayed immediately, samples can be stored at -80°C.
  • In order to compare the hydroxyl radical scavenging capacity of different samples, serum, tissue homogenate, juice and other liquid samples must add the same volume for the same batch. Exact or Drug must be diluted to the same concentration.
• Storage instructions

Storage at 4°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.

• Shipping

Gel pack with blue ice.

 活細胞和死細胞雙染色套組  |  CCK-8細胞增殖和細胞毒性試劑盒  | LDH細胞毒性測定試劑盒 | 細胞衰老檢測試劑盒（β-半乳糖苷酶 | 細胞增殖EdU Image試劑盒 

Catalog no	product name
KTB1010	CheKine™ NADP/NADPH Assay Kit
KTB1011	CheKine™ Glucose-6-Phosphate Dehydrogenase (G6PDH) Activity Colorimetric Assay Kit
KTB1020	CheKine™ NAD/NADH Assay Kit
KTB1022	CheKine™ NAD+ kinase (NADK) Activity Colorimetric Assay Kit
KTB1030	CheKine™ Superoxide Dismutases (SOD) Assay Kit
KTB1040	CheKine™ Catalase (CAT) Activity Assay Kit
KTB1050	CheKine™ Lipid Peroxidation (MDA) Assay kit
KTB1060	CheKine™ Peroxidase (HRP) Assay Kit
KTB1070	CheKine™ Xanthine Oxidase Assay Kit
KTB1080	CheKine™ Superoxide anion Scavenging Capacity Assay Kit
KTB1090	CheKine™ Hydroxyl Free Radical Scavenging Capacity Assay Kit
KTB1100	CheKine™ Lactate Assay Kit
KTB1110	CheKine™ Lactate Dehydrogenase (LDH) Assay Kit
KTB1300	CheKine™ Glucose Assay Kit
KTB1310	CheKine™ Glucose Oxidase Activity (GOD) Assay Kit
KTB1041	CheKine™ Hydrogen Peroxide (H2O2) Assay Kit
KTB1400	CheKine™ Nitric Oxide (NO) Assay Kit
KTB1500	CheKine™ Total Antioxidant Capacity (TAC) Assay Kit
KTB1600	CheKine™ Reduced Glutathione (GSH) Colorimetric Assay Kit
KTB1610	CheKine™ Glutathione Oxidized (GSSG) Colorimetric Assay Kit
KTB1620	CheKine™ Glutathione Reductases (GR) Colorimetric Assay Kit
KTB1630	CheKine™ Glutathione S-Transferase (GST) Colorimetric Assay Kit
KTB1640	CheKine™ Glutathione Peroxidase (GSH-Px) Colorimetric Assay Kit
KTB1650	CheKine™ Thioredoxin Reductase (TrxR) Colorimetric Assay Kit
KTB1660	CheKine™ Thioredoxin Peroxidase (TPX) Colorimetric Assay Kit
KTB1021	CheKine™ NADH Oxidase (NOX) Colorimetric Assay Kit
KTB1120	CheKine™ Pyruvate Kinase (PK) Colorimetric Assay Kit
KTB1200	CheKine™ Protein Carbonyl Colorimetric Assay Kit
KTB1700	CheKine™ Tissue and Blood Alkaline Phosphatase (AKP/ALP) Colorimetric Assay Kit
KTB1800	CheKine™ Na⁺/K⁺-ATPase Activity Colorimetric Assay Kit
KTB1810	CheKine™ Ca2⁺/Mg2⁺-ATPase Activity Colorimetric Assay Kit
KTB1900	CheKine™ Monoamine Oxidase (MAO) Activity Colorimetric Assay Kit
KTB1140	CheKine™ Polyphenol Oxidase (PPO) Activity Colorimetric Assay Kit
KTB2100	CheKine™ Serum Potassium (K+) Colorimetric Assay Kit
KTB2200	CheKine™ Triglyceride (TG) Colorimetric Assay Kit
KTB2210	CheKine™ Free Cholesterol (FC) Colorimetric Assay Kit
KTB2220	CheKine™ Total Cholesterol (TC) Colorimetric Assay Kit