總膽固醇呈色螢光分析套組 |Total Cholesterol and Cholesteryl Ester Assay Kit 貨號, K603-100 (ab65359)

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總膽固醇呈色螢光分析套組 Total Cholesterol and Cholesteryl Ester Colorimetric/Fluorometric Assay Kit

總膽固醇呈色螢光分析套組 | 貨號K603-100 (ABCAM ab65359)

100個分析 Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)

產品描述: Biovision提供一系列脂質分析套組；其中膽固醇/膽固醇酯呈色螢光分析套組 (Cholesterol/Cholesteryl Ester Quantitation, K603)，一組產品可選擇呈色Absorbance (570 nm)或螢光Fluorescence (Ex/Em 535/587 nm)兩種型態偵測；可用於細胞培養液、尿液、血漿、或液體型式的來源。操作時間大約60分鐘。可測量總膽固醇、游離膽固醇、膽固醇酯之含量。血液中的大部分膽固醇是膽固醇酯 (cholesteryl esters )形式，其可以通過膽固醇酯酶 (cholesterol esterase)水解成膽固醇。然後膽固醇( cholesterol )被膽固醇氧化酶( cholesterol oxidase )氧化，產生H 2 O 2，其與敏感的膽固醇探針反應產生顏色（λmax= 570nm）和螢光（Ex / Em = 535 / 587nm）。該試驗在反應中不存在膽固醇酯酶的情況下，在膽固醇酯酶或游離膽固醇存在下檢測總膽固醇 ( total cholesterol)【膽固醇 (cholesterol )和膽固醇酯 ( cholesteryl esters)】。膽固醇酯可以通過從總量（膽固醇加膽固醇酯）中減去游離膽固醇(free cholesterol )的值來確定。

Total Cholesterol and Cholesteryl Ester Colorimetric/Fluorometric Assay Kit | BioVision Inc.

The assay detects total cholesterol (cholesterol and cholesteryl esters) in the presence of cholesterol esterase or free cholesterol in the absence of cholesterol esterase in the reaction.

膽固醇呈色螢光分析套組 | K603-100內容物
• Cholesterol Assay Buffer
• Cholesterol Probe (in DMSO, anhydrous)
• Enzyme Mix (lyophilized)
• Cholesterol Esterase (lyophilized)
• Cholesterol Standard (2 µg/µl)

Cat # +Size	K603-100 (ABCAM ab65359)
Size	100 assays
Detection Method	Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
Species Reactivity	Mammalian
Applications	The assay detects total cholesterol (cholesterol and cholesteryl esters) in the presence of cholesterol esterase or free cholesterol in the absence of cholesterol esterase in the reaction.
Features & Benefits	• Simple procedure; takes ~60 minutes • Fast and convenient
Kit Components	• Cholesterol Assay Buffer • Cholesterol Probe (in DMSO, anhydrous) • Enzyme Mix (lyophilized) • Cholesterol Esterase (lyophilized) • Cholesterol Standard (2 µg/µl)

Sara Della Torre, et al., (2018) Short-Term Fasting Reveals Amino Acid Metabolism as a Major Sex-Discriminating Factor in the Liver, Cell Metabolism, Aug. 2018, 29909969

Ana I. Duarte, et al., (2018) Dual Therapy with Liraglutide and Ghrelin Promotes Brain and Peripheral Energy Metabolism in the R6/2 Mouse Model of Huntington’s Disease, Scientific Reports, Jun. 2018, 29895889

Zhang P et al., (2017) Beraprost sodium, a prostacyclin analogue, reduces fructose-induced hepatocellular steatosis in mice and in vitro via the microRNA-200a and SIRT1 signaling pathway, Metabolism, 2017, 73:9-21

Tse, Margaret Chui Ling et al. (2017) Tumor Necrosis Factor a Promotes Phosphoinositide 3-Kinase Enhancer A and Amp-Activated Protein Kinase Interaction to Suppress Lipid Oxidation in Skeletal Muscle, Diabetes. 2017 Jul;66(7):1858-1870.

Huang, Er-Wen et al. (2016) Endophilin-A2-mediated increase in scavenger receptor expression contributes to macrophage-derived foam cell formation, Atherosclerosis. 2016 Nov;254:133-141.

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