粒線體萃取試劑盒 | ExKine™ Mitochondrion Extraction Kit (Tissue) 貨號KTP4004

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原廠連結：	http://biopioneer.com.tw
相關下載：	粒線體(Mitochondria)萃取試劑盒操作手冊

特點

粒線體(Mitochondria)萃取試劑盒 | ExKine™ Mitochondrion Extraction Kit (Tissue) | 貨號KTP4004

Abbkine ExKine™粒線體(Mitochondria)提取試劑盒(KTP4004) ExKine™ Mitochondrion Extraction Kit,（組織）能夠使用差速離心法從軟組織和硬組織中從動物組織中快速粗略地分離完整的粒線體(Mitochondria)。這種粗粒線體(Mitochondria)製劑通常足以用於大多數應用，例如粒線體(Mitochondria)呼吸(mitochondrial respiration)、線粒體膜電位(mitochondria membrane potential)、細胞凋亡(apoptosis)、mtDNA 和 mtRNA 以及線粒體蛋白質分析(mitochondrial protein profiling)等研究。線粒體(Mitochondria)是在大多數真核細胞中發現的雙膜結合細胞器(double-membrane-bound organelle)。線粒體(Mitochondria)的功能是提供細胞能量。此外，線粒體(Mitochondria)還參與其他任務，例如信號傳導(signaling)、細胞分化(cellular differentiation)和細胞死亡(cell death)，以及維持對細胞週期(cell cycle)和細胞生長(cell growth)的控制。線粒體(Mitochondria)與多種人類疾病有關，包括線粒體疾病(mitochondrial disorders)、心臟功能障礙(cardiacdysfunction)、心力衰竭(heart failure)和自閉症(autism)。線粒體可能在這些細胞過程中發揮重要作用。

$Fig. Mouse liver tissues were extracted with ExKine™ Mitochondrion Extraction Kit (Tissue). Mitochondrial (M) and Cytosolic (C) fractions were analyzed by WB using cytochrome c antibody (cat#ABM40191) and VDAC1 antibody (cat#ABP53121).$

Fig. Mouse liver tissues were extracted with ExKine™ Mitochondrion Extraction Kit (Tissue). Mitochondrial (M) and Cytosolic (C) fractions were analyzed by WB using cytochrome c antibody (cat#ABM40191) and VDAC1 antibody (cat#ABP53121).

Kit components	• Lysis Buffer A (5×) • Lysis Buffer B (5×) • Storage Buffer
Features & Benefits	• Isolate intact mitochondria from animal tissues from both soft and hard tissues. • Isolated mitochondria is compatible with almost any downstream application.
Usage notes	Perform all steps at 2–8 °C. Use precooled buffers and equipment. Ensure all the solutions are defrosted and homogeneous.
Storage instructions	Refer to list of materials supplied for storage conditions of individual components. Stable for at least 12 months at recommended temperature from date of shipment. Gel pack with blue ice.
Shipping	Gel pack with blue ice.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Product Type		Catalog No	Calibration Range	Limit of detection
ELISA Assay Kit	EliKine™ Human CCL2 ELISA Kit	KET6001	62.5 pg/ml-4000 pg/ml	30 pg/mL
ELISA Assay Kit	EliKine™ Human CCL3 ELISA Kit	KET6002	31.25 pg/mL- 2000 pg/mL	16 pg/Ml
ELISA Assay Kit	EliKine™ Human sICAM-1 (CD54) ELISA Kit	KET6003	7.81 pg/mL- 500 pg/mL	7.81 pg/mL
ELISA Assay Kit	EliKine™ Human CRP ELISA Kit	KET6004	15.6 pg/mL-1000 pg/mL	8 pg/mL
ELISA Assay Kit	EliKine™ Human EGF ELISA Kit	KET6005	3.9 pg/mL-250 pg/mL	2 pg/Ml
ELISA Assay Kit	EliKine™ Human bFGF ELISA Kit	KET6006	15.6 pg/mL-1000 pg/mL	8 pg/mL
ELISA Assay Kit	EliKine™ Human G-CSF ELISA Kit	KET6007	31.25 pg/mL-2000 pg/mL	16 pg/mL
ELISA Assay Kit	EliKine™ Human GM-CSF ELISA Kit	KET6008	11.72 pg/mL-750 pg/mL	5 pg/mL
ELISA Assay Kit	EliKine™ Human HGF ELISA Kit	KET6009	125 pg/mL-8000 pg/mL	60 pg/mL
ELISA Assay Kit	EliKine™ Human IFN-α ELISA Kit	KET6010	46.9 pg/mL – 3000 pg/mL	46.9 pg/mL
ELISA Assay Kit	EliKine™ Human IFN-γ ELISA Kit	KET6011	7.8 pg/mL-500 pg/mL	4 pg/mL
ELISA Assay Kit	EliKine™ Human IL-1α ELISA Kit	KET6012	7.8 pg/mL-500 pg/mL	4 pg/mL
ELISA Assay Kit	EliKine™ Human IL-1β ELISA Kit	KET6013	7.8 pg/ml-500 pg/ml	4 pg/mL
ELISA Assay Kit	EliKine™ Human IL-2 ELISA Kit	KET6014	3.9 pg/ml-250 pg/ml	2 pg/ml
ELISA Assay Kit	EliKine™ Human IL-4 ELISA Kit	KET6015	3.125 pg/mL- 200 pg/mL	1.5 pg/mL
ELISA Assay Kit	EliKine™ Human IL-5 ELISA Kit	KET6016	7.8 pg/mL-500 pg/mL	4 pg/mL
ELISA Assay Kit	EliKine™ Human IL-6 ELISA Kit	KET6017	3.125 pg/ml-200 pg/ml	2 pg/mL
ELISA Assay Kit	EliKine™ Human IL-8 ELISA Kit	KET6018	3.9 pg/ml-250 pg/ml	2 pg/mL
ELISA Assay Kit	EliKine™ Human IL-10 ELISA Kit	KET6019	2.35 pg/ml-150 pg/ml	1 pg/mL
ELISA Assay Kit	EliKine™ Human IL-12 p70 ELISA Kit	KET6020	7.8 pg/mL-500 pg/mL	4 pg/mL
ELISA Assay Kit	EliKine™ Human IL-13 ELISA Kit	KET6021	7.8 pg/mL-500 pg/mL	4 pg/mL
ELISA Assay Kit	EliKine™ Human IL-17 ELISA Kit	KET6022	7.8 pg/mL-500 pg/mL	4 pg/mL
ELISA Assay Kit	EliKine™ Human IL-18 ELISA Kit	KET6023	156 pg/mL- 10000 pg/mL	40 pg/mL
ELISA Assay Kit	EliKine™ Human IL-22 ELISA Kit	KET6024	15.6 pg/mL-1000 pg/mL	8 pg/mL
ELISA Assay Kit	EliKine™ Human IL-23 ELISA Kit	KET6025	31.25 pg/mL-2000 pg/mL	16 pg/mL
ELISA Assay Kit	EliKine™ Human Leptin ELISA Kit	KET6026	31.25 pg/mL- 2000 pg/mL	16 pg/mL
ELISA Assay Kit	EliKine™ Human MMP-9 ELISA Kit	KET6027	31.25 pg/mL- 2000 pg/mL	16 pg/mL
ELISA Assay Kit	EliKine™ Human β-NGF ELISA Kit	KET6028	31.25 pg/mL- 2000 pg/mL	16 pg/mL
ELISA Assay Kit	EliKine™ Human Resistin ELISA Kit	KET6029	31.25 pg/mL-2000 pg/mL	16 pg/mL
ELISA Assay Kit	EliKine™ Human TGF-β1 ELISA Kit	KET6030	15.6 pg/ml-1000 pg/ml	8 pg/mL
ELISA Assay Kit	EliKine™ Human TIMP-1 ELISA Kit	KET6031	31.25 pg/ml-2000 pg/ml	16 pg/mL
ELISA Assay Kit	EliKine™ Human TNF-α ELISA Kit	KET6032	7.8 pg/ml-500 pg/ml	4 pg/mL
ELISA Assay Kit	EliKine™ Human VEGF ELISA Kit	KET6033	31.25 pg/mL-2000 pg/mL	16 pg/mL
ELISA Assay Kit	EliKine™ Human IgG ELISA Kit	KET6034	0.125 ng/mL- 8 ng/mL	0.1 ng/mL
ELISA Assay Kit	EliKine™ Mouse CCL2 ELISA Kit	KET7001	31.25 pg/mL-2000 pg/mL	16 pg/mL
ELISA Assay Kit	EliKine™ Mouse GM-CSF ELISA Kit	KET7002	7.8 pg/mL-500 pg/mL	4 pg/mL
ELISA Assay Kit	EliKine™ Mouse IFN-γ ELISA Kit	KET7003	31.25 pg/ml- 2000 pg/ml	16 pg/ml
ELISA Assay Kit	EliKine™ Mouse IL-1α ELISA Kit	KET7004	7.8 pg/mL-500 pg/mL	4 pg/mL
ELISA Assay Kit	EliKine™ Mouse IL-1β ELISA Kit	KET7005	15.6 pg/ml-1000 pg/ml	8 pg/mL
ELISA Assay Kit	EliKine™ Mouse IL-2 ELISA Kit	KET7006	3.13 pg/mL- 200 pg/mL	1.6 pg/mL
ELISA Assay Kit	EliKine™ Mouse IL-4 ELISA Kit	KET7007	Null	Null
ELISA Assay Kit	EliKine™ Mouse IL-5 ELISA Kit	KET7008	7.8 pg/mL-500 pg/mL	4 pg/mL
ELISA Assay Kit	EliKine™ Mouse IL-6 ELISA Kit	KET7009	7.82 pg/mL-500 pg/mL	2 pg/mL
ELISA Assay Kit	EliKine™ Mouse IL-10 ELISA Kit	KET7010	93.75 pg/mL- 6000 pg/mL	50 pg/mL
ELISA Assay Kit	EliKine™ Mouse IL-12 p70 ELISA Kit	KET7011	31.25 pg/mL- 2000 pg/mL	16 pg/mL
ELISA Assay Kit	EliKine™ Mouse IL-17 ELISA Kit	KET7012	7.8 pg/mL-500 pg/mL	4 pg/mL
ELISA Assay Kit	EliKine™ Mouse IL-22 ELISA Kit	KET7013	15.6 pg/mL-1000 pg/mL	8 pg/mL
ELISA Assay Kit	EliKine™ Mouse TGF-β1 ELISA Kit	KET7014	15.6 pg/ml-1000 pg/ml	8 pg/mL
ELISA Assay Kit	EliKine™ Mouse TNF-α ELISA Kit	KET7015	15.6 pg/ml-1000 pg/ml	8 pg/mL
ELISA Assay Kit	EliKine™ Mouse VEGF ELISA Kit	KET7016	15.6 pg/ml-1000 pg/ml	8 pg/ml
ELISA Assay Kit	EliKine™ Rat Interferon Gamma (IFN-γ) ELISA Kit	KET7017	150 pg/mL-2400 pg/mL	15 pg/mL
ELISA Assay Kit	EliKine™ Mouse IgG ELISA Kit	KET7018	0.5 ng/mL- 32 ng/mL	0.5 ng/mL
ELISA Assay Kit	EliKine™ Rat IL-1β ELISA Kit	KET9001	62.5 pg/ml-4000 pg/ml	32 pg/mL
ELISA Assay Kit	EliKine™ Rat IL-2 ELISA Kit	KET9002	Null	Null
ELISA Assay Kit	EliKine™ Rat IL-4 ELISA Kit	KET9003	1.56 pg/mL-100 pg/mL	1.0 pg/mL
ELISA Assay Kit	EliKine™ Rat IL-6 ELISA Kit	KET9004	250pg/mL-16000 pg/mL	100 pg/mL
ELISA Assay Kit	EliKine™ Rat IL-17 ELISA Kit	KET9005	3.13 pg/mL-200 pg/mL	2 pg/mL
ELISA Assay Kit	EliKine™ Rat TGF-β1 ELISA Kit	KET9006	15.6 pg/mL-1000 pg/mL	8 pg/mL
ELISA Assay Kit	EliKine™ Rat TNF-α ELISA Kit	KET9007	31.25 pg/mL- 2000 pg/mL	16 pg/ml
ELISA Assay Kit	EliKine™ Rat VEGF ELISA Kit	KET9008	15.6 pg/ml-1000 pg/ml	8 pg/mL
ELISA Assay Kit	EliKine™ Human β-hCG ELISA Kit	KET6901	8 IU/L-240 IU/L	2.0 IU/L
ELISA Assay Kit	EliKine™ Human LH ELISA Kit	KET6902	2 IU/L-75 IU/L	0.5 IU/L
ELISA Assay Kit	EliKine™ Human FSH ELISA Kit	KET6903	2 IU/L-70 IU/L	1.0 IU/L
ELISA Assay Kit	EliKine™ Human Prolactin ELISA Kit	KET6904	40 μIU/ml-2000 μIU/ml	50 μIU/ml
ELISA Assay Kit	EliKine™ Human TSH ELISA Kit	KET6905	0.3 mIU/L-12 mIU/L	0.1 mIU/L
ELISA Assay Kit	EliKine™ Testosterone ELISA Kit	KET0001	0.1 ng/ml-20 ng/ml	0.1 ng/ml
ELISA Assay Kit	EliKine™ Progesterone ELISA Kit	KET0002	0.5 ng/ml-30 ng/ml	0.2 ng/ml
ELISA Assay Kit	EliKine™ Estradiol ELISA Kit	KET0003	40 pg/ml-1500 pg/ml	40 pg/ml
ELISA Assay Kit	EliKine™ Free Triiodothyronine (fT3) ELISA Kit	KET0004	2 pmol/L-32 pmol/L	2.0 pmol/L
ELISA Assay Kit	EliKine™ Free Thyroxine (fT4) ELISA Kit	KET0005	4 pmol/L-64 pmol/L	4.0 pmol/L
ELISA Assay Kit	EliKine™ Triiodothyronine (T3) ELISA Kit	KET0006	0.5 ng/ml-8 ng/ml	0.4 ng/ml
ELISA Assay Kit	EliKine™ Thyroxine (T4) ELISA Kit	KET0007	20 ng/ml-320 ng/ml	10 ng/ml
ELISA Assay Kit	EliKine™ Human PD-1 ELISA Kit	KET6035	3.9 pg/mL – 250 pg/mL	3.9 pg/mL
ELISA Assay Kit	EliKine™ Human PD-L1 ELISA Kit	KET6036	12.5 pg/mL – 800 pg/mL	6.25 pg/mL

產品貨號	品項	反應	測量儀器
貨號KTA1001	活細胞和死細胞雙染色試劑盒	100個反應	活細胞綠色螢光染劑Ex/Em = 488/530 nm; 死細胞紅色螢光染料(Ex/Em = 535/617)
貨號 KTA2010	TUNEL細胞凋亡檢測試劑盒	50個反應	螢光酶標儀、螢光顯微鏡或流式細胞儀。FITC通道（Ex / Em = 490nm / 520 nm）
貨號 KTC011001	CCK-8細胞增殖和細胞毒性試劑盒	500個反應	吸收光450nm
貨號KTC4003	TraKine™粒線體染色	50個反應/250個反應	Ex / Em = 490/523 nm增殖(proliferating)和非增殖細胞(non-proliferating cells)
貨號KTC4100	Pro活細胞微管蛋白染色	50個反應/250個反應	Ex/Em = 500/520 nm
貨號KTC4001	Abbkine 細胞膜染色	100個反應/500個反應	Ex/Em = 484/501 nm
貨號BMD00063	DAPI Staining Solution	10MG/100MG	Ex/Em: 358/461 nm (with DNA)
貨號KTC4210	活細胞Lysosome染色	50個反應/250個反應	deep red fluorescent dye , Ex/Em:650/665nm
貨號KTA3030	細胞衰老檢測試劑盒（β-半乳糖苷酶）	100個反應	光學顯微鏡
貨號KTA1020	CCK-8細胞增殖和細胞毒性試劑盒	1000個反應/10,000個反應	吸收光450nm
貨號KTA2030	細胞增殖EdU Image試劑盒 (BrdU替代法)	100個反應/500個反應	AbFluor 488 azide (Ex/Em = 501/525 nm)
貨號KTB1400	一氧化Dan（NO）檢測試劑盒	96個反應/ 192個反應	colorimetric (540 nm) 檢測範圍：1-100 µM
貨號KTB1070	CheKine™黃嘌呤氧化酶檢測	96個反應/ 480個反應	OD 450 nm 線性範圍15.6 -500 mU/
貨號KTD3001	BCA蛋白定量試劑盒	2000個反應/ 5000個反應	OD 562 nm 相容性 5 % SDS, 5 % Triton X-100, 5 % Tween-20, 60, 80 50 to 1000 µg/ml.
貨號KTB1110	乳酸脫氫酶（LDH）測定試劑盒	96個反應/ 480個反應	λmax= 450 nm 1- 20 U/mL of LDH
貨號PRP100153	IGF-I重組蛋白	200UG/1MG	human IGF1 isoform 1 (P05019-1) (Gly 49-Ala 118) > 95 % as determined by SDS-PAGE
貨號 KTA2020	細胞週期染色檢測	100個反應/500個反應	Ex/Em=535/615 nm.
貨號BMC21400	PurKine™內毒素去除預裝離心柱	1ml×5 3mlx5	More than 2,000,000 EU/mL to eliminate >99% of endotoxins.
貨號KTA1200	CheKine™蛋白羰基比色測定試劑盒	48個反應/ 96個反應	吸收光370 nm
貨號KTB1080	超氧陰離子清除測定試劑盒	48個反應/ 96個反應	Superoxide anion scavenging rate D% =（∆∆ODControl – ∆∆ODSample）÷ ∆∆ODControl ×100%
貨號KTB1090	清除氫氧自由基能力測定試劑	48個反應/ 96個反應	吸收光 536nm Hydroxyl radical scavenging rate D% =（ASample – AControl）÷（ABlank – AControl）×100%
貨號KTB2220	TC總膽固醇檢測試劑盒	48個反應/ 96個反應	500nm Standard: 5 μmol/mL
貨號KTB1510	CheKine™尿酸（UA）比色分析試劑盒	48個反應/ 96個反應	吸收光505 nm Standard: 5 μmol/mL.
貨號KTB1300	Glucose葡萄糖測定試劑盒	96個反應/ 192個反應	吸光值630nm 4- 300 mg/dL)
貨號KTB1100	Lactate 乳酸檢測試劑盒	96個反應/ 480個反應	colorimetric (450 nm) 0.03 mM-2 mM
貨號KTB1700	組織和血液鹼性磷酸酶（ Alkaline Phosphatase, AKP / ALP）比色測定	96個反應/ 96 X5盤	吸收光510 nm
貨號KTB1500	總抗氧化劑能力TAC分析	96個反應/ 480個反應	593 nm 偵測範圍: 0.15-3 mM
貨號KTA0002	Annexin V-AbFluor™488細胞凋亡檢測試劑盒	20個反應/50個反應/100個反應	Annexin V- AbFluor™ 488: Abs/Em = 491/517 nm; PI: Abs/Em = 535/617 nm (with DNA)
貨號KET7013	EliKine™ Rat IL-6 ELISA Kit試劑盒	96個反應/ 96 X5盤	450 nm 偵測範圍: 250pg/mL-16000 pg/mL, 最低靈敏度100 pg/mL
貨號KET0003	雌二醇（Estradiol , E2）試劑盒	96個反應/ 96 X5盤	450 nm 偵測範圍40 pg/ml-1500 pg/ml
貨號KET6032	TNF–α Alpha型腫瘤壞死因子 ELISA試劑盒	96個反應/ 96 X5盤	450 nm 7.8 pg/ml-500 pg/ml
貨號KET6033	VEGF血管內皮生長因子ELISA試劑盒	96個反應/ 96 X5盤	450 nm 偵測範圍: 31.25 pg/mL-2000 pg/mL
貨號KET7005	白介素-1β（Interleukin-1β, IL-1β）ELISA試劑盒	96個反應/ 96 X5盤	Microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm. 偵測範圍: 15.6 pg/ml-1000 pg/ml
貨號KTE70003	小鼠黃嘌呤脫氫酶/氧化酶（XDH）ELISA試劑盒	96個反應/ 96 X5盤	450 nm 偵測範圍: 22.5 ng/L-360 ng/L, 最低靈敏度1.0 ng/L。
貨號 A02051HRP	His-tag標籤多株抗體-HRP	50ul/200ul	WB (1:5000).
貨號A02170MGB	Anti-V5 Tag Mouse Monoclonal Antibody (11D5), 磁珠	100ul/400ul/2ml	GKPIPNPLLGLDST epitope
貨號KTA4001	JC-1線粒體膜電位測定試劑盒	100個反應/ 500個反應	（聚結的JC-1，Ex / Em = 585/590 nm）發出綠色螢光（JC-1單體，Ex / Em = 510/527 nm） JC-1 Stain CCCP (10 mM) Assay Buffer (5×)
貨號KTB1600	穀胱甘肽(Glutathione)檢測	96個反應	412 nm 2-200 µg/mL
貨號BMD00063	DAPI Staining Solution	1MG/10MG/100MG	λEx/λEm: 358/461 nm (with DNA)
貨號KTP3001	細胞核/細胞質分離試劑盒	50個反應/ 200個反應	Cytoplasmic Solution A (CES A) Cytoplasmic Solution B (CES B) • Nuclear Extraction Solution (NES) DTT (500X) Protease Inhibitor (100X)
貨號BMD00064-50UG	Calcein AM鈣黃綠素活細胞螢光染劑	50ug/1mg/10mg	λEX/λEm: 494/517 nm (pH 8) (calcein)
貨號 KTB1660	硫氧還蛋白過氧化物酶（TPX）檢測	96個反應	240nm
貨號KTB1020	NAD/NADH 分析試劑盒	96個反應/ 480個反應	OD565 nm 96 孔板測定中的檢測限值為 0.78 μM 線性最高 50 μM NAD+/NADH。
貨號KTB1070	CheKine™黃嘌呤氧化酶檢測	96個反應/ 480個反應	OD 450 nm 線性範圍15.6 -500 mU/mL
貨號KTB1040	Catalase過氧化氫酶活性檢測試劑盒	96個反應/ 480個反應	OD 540 nm 2-75 µM
貨號KTB1060	過氧化物酶（Peroxidases）分析試劑盒	96個反應	OD 664 nm 線性範圍為15.6-1000 µU/mL
貨號KTB1050	脂質過氧化檢測試劑盒(MDA)	96個反應	OD 532 nm 1-100 µM/L
貨號KTB1030	超氧化物歧化酶（SOD）活性測定試劑盒	96個反應	200-3.13 U/ml
貨號KTA1030	LDH細胞毒性測定試劑盒	96個反應/ 480個反應	OD 565 nm 10,00-100,0000 cells per well

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